scholarly journals Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay

2012 ◽  
Vol 31 (6) ◽  
pp. 1028-1032 ◽  
Author(s):  
Zeliha YILMAZ ◽  
A. Lale DOGAN ◽  
Ozgur OZDEMIR ◽  
Ahmet SERPER
2020 ◽  
Vol 22 (3) ◽  
Author(s):  
Maria Szymonowicz ◽  
_ Agnieszka ◽  
Magdalena Pajączkowska ◽  
Joanna Nowicka ◽  
Kamila Wiśniewska ◽  
...  

Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.


2009 ◽  
Vol 293 (1) ◽  
pp. 102-106 ◽  
Author(s):  
Vojtech BoldiÅ¡ ◽  
Jasna Å trus ◽  
Elena Kocianová ◽  
Magda TuÅ¡ek-Žnidarič ◽  
Katarína Å tefanidesová ◽  
...  

2009 ◽  
Vol 17 (5) ◽  
pp. 457-461 ◽  
Author(s):  
Gabriela Tavares Bekner Correa ◽  
Gabriel Alves Costa Veranio ◽  
Licínio Esmaeraldo Silva ◽  
Raphael Hirata Junior ◽  
Jeffry M. Coil ◽  
...  

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