scholarly journals Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study

2020 ◽  
Vol 22 (3) ◽  
Author(s):  
Maria Szymonowicz ◽  
_ Agnieszka ◽  
Magdalena Pajączkowska ◽  
Joanna Nowicka ◽  
Kamila Wiśniewska ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
In Vitro Study ◽  
L929 Cell ◽  
Aluminium Chloride ◽  
Srb Assay ◽  
Haemostatic Agents ◽  
L929 Cell Line

Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.

In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Chemosphere ◽  
2017 ◽  
Vol 173 ◽  
pp. 452-459 ◽  
Author(s):  
Teuta Eljezi ◽  
Pierre Pinta ◽  
Damien Richard ◽  
Jérémy Pinguet ◽  
Jean-Michel Chezal ◽  
...  
Keyword(s):  
Cell Line ◽  
L929 Cell ◽  
Cytotoxic Effects ◽  
Primary Metabolites ◽  
L929 Cell Line

scholarly journals Evaluation of the cytotoxicity of different root canal sealers on L929 cell line by MTT assay

2012 ◽  
Vol 31 (6) ◽  
pp. 1028-1032 ◽  
Author(s):  
Zeliha YILMAZ ◽  
A. Lale DOGAN ◽  
Ozgur OZDEMIR ◽  
Ahmet SERPER
Keyword(s):  
Cell Line ◽  
Mtt Assay ◽  
Root Canal ◽  
L929 Cell ◽  
Root Canal Sealers ◽  
L929 Cell Line

scholarly journals Synthesis of New Pt(II) Complex Bearing Organoselenium Ligands and Evaluation of Cytotoxic Activity of Some Structurally Related Pd(II) Complexes

2020 ◽  
Vol 39 (1) ◽  
pp. 59
Author(s):  
Ninko Radenković ◽  
Marina Kostić ◽  
Nataša Đorđević ◽  
Zana Doličanin ◽  
Tanja Soldatović ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
Carcinoma Cell ◽  
In Vitro Study ◽  
Colorectal Carcinoma Cell Line ◽  
Hct 116 ◽  
Healthy Lung

Herein we report the synthesis of new trans-bis(2-phenylselenylmethyl)oxolane)dichloroplatinum(II) complex Pt1. Newly synthetized complex, together with two, structurally related trans-Pd(II) complexes (trans-bis(2-(phenylselenylmethyl)oxolane)dichloropalladium(II) Pd1 and trans-bis(2-(phenylselenylmethyl)oxane)dichloropalladium(II)) Pd2, were screened for cytotoxic activity through in vitro study on the HCT-116 colorectal carcinoma cell line and on the MRC-5 healthy lung pleura cell line. Activity of complexes was assessed by comparing it to the cisplatin. The cells viability and proliferation were determined using MTT assay.

scholarly journals In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Vincenza Cannella ◽  
Roberta Altomare ◽  
Vincenza Leonardi ◽  
Laura Russotto ◽  
Santina Di Bella ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Medical Devices ◽  
L929 Cell ◽  
Cell Number ◽  
Proliferation Rate ◽  
Two Samples ◽  
Human Use ◽  
L929 Cell Line

Objective. Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in patients following the injection. Biocompatibility assays are required for medical devices intended for human use, in order to prevent damages or injuries in the host. In this study, nine HA fillers were tested in order to evaluate their cytotoxicity and their effects on L929 cell line, according to the UNI EN ISO 10993 regulation. Methods. Extracts were prepared from nine HA fillers, and MTS viability assay was performed after 24 h, 48 h, and 72 h of exposure of cells to extracts. Cells cultured with HA filler extracts were monitored for up to 72 h, counted, and stained with haematoxylin/eosin in order to evaluate the cell proliferation rate and morphology. Results. None of the filler tested showed a cytotoxic effect. Two samples showed a higher vitality percentage and higher cell number while two samples showed a lower vitality percentage and lower cell number at 72 h. Conclusion. Data obtained suggest that although examined fillers are not cytotoxic, they show different effects on the in vitro cell proliferation rate. In vitro studies of medical devices could lead to important implications since these could aid to predict effects about their in vivo application. These easy and rapid assays could be useful to test new materials intended for human use avoiding animal tests.

scholarly journals Enhancing Anti-Tumoral Potential of CD-NHF by Modulating PI3K/Akt Axis in U87 Ex Vivo Glioma Model

2021 ◽  
Vol 22 (8) ◽  
pp. 3873
Author(s):  
Gabriel Luta ◽  
Mihail Butura ◽  
Adrian Tiron ◽  
Crina E. Tiron
Keyword(s):  
Cell Line ◽  
Ex Vivo ◽  
Glioma Cells ◽  
In Vitro Study ◽  
Immunofluorescent Staining ◽  
Expression Levels ◽  
Disease Diagnostics ◽  
Phosphorylated Akt ◽  
Significant Impairment

Background: In the latest years, there has been an increased interest in nanomaterials that may provide promising novel approaches to disease diagnostics and therapeutics. Our previous results demonstrated that Carbon-dots prepared from N-hydroxyphthalimide (CD-NHF) exhibited anti-tumoral activity on several cancer cell lines such as MDA-MB-231, A375, A549, and RPMI8226, while U87 glioma tumor cells were unaffected. Gliomas represent one of the most common types of human primary brain tumors and are responsible for the majority of deaths. In the present in vitro study, we expand our previous investigation on CD-NHF in the U87 cell line by adding different drug combinations. Methods: Cell viability, migration, invasion, and immunofluorescent staining of key molecular pathways have been assessed after various treatments with CD-NHF and/or K252A and AKTVIII inhibitors in the U87 cell line. Results: Association of an inhibitor strongly potentiates the anti-tumoral properties of CD-NHF identified by significant impairment of migration, invasion, and expression levels of phosphorylated Akt, p70S6Kinase, or by decreasing expression levels of Bcl-2, IL-6, STAT3, and Slug. Conclusions: Using simultaneously reduced doses of both CD-NHF and an inhibitor in order to reduce side effects, the viability and invasiveness of U87 glioma cells were significantly impaired.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

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