Purification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008

2010 ◽  
Vol 20 (6) ◽  
pp. 1001-1005 ◽  
Author(s):  
Tzu-Wen Liang
Keyword(s):  
New Species ◽  
Pseudomonas Sp ◽  
Purification And Characterization ◽  
A New Species

scholarly journals Isolation, Purification, and Characterization of Xylanase Produced by a New Species ofBacillusin Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Rajashri D. Kamble ◽  
Anandrao R. Jadhav
Keyword(s):  
New Species ◽  
Solid State ◽  
Solid State Fermentation ◽  
Exchange Chromatography ◽  
Optimum Temperature ◽  
Birchwood Xylan ◽  
Purification And Characterization ◽  
Application Potential ◽  
A New Species

A thermoalkalophilic new species ofBacillus, similar toBacillus arseniciselenatisDSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was ~29.8 ;kDa. The optimum temperature and pH for the enzyme activity were 50°C and pH 8.0. The enzyme was active on birchwood xylan and little active onp-nitrophenyl xylopyranoside but not on Avicel, CMC, cellobiose, and starch, showing its absolute substrate specificity. For birchwood xylan, the enzyme gave a Km 5.26 ;mg/mL and Vmax 277.7 ;μmol/min/mg, respectively. In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp biobleaching processes but also in the nutraceutical industry.

Ultrastructural and molecular characterization of Bacillidium vesiculoformis n. sp. (Microspora: Mrazekiidae) in the freshwater oligochaete Nais simplex (Oligochaeta: Naididae)

Parasitology ◽  
2004 ◽  
Vol 130 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. J. MORRIS ◽  
R. S. TERRY ◽  
K. B. FERGUSON ◽  
J. E. SMITH ◽  
A. ADAMS
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Infected Cell ◽  
Host Cell ◽  
Developmental Stages ◽  
Parasite Development ◽  
Adhesive Quality ◽  
Transmission Studies ◽  
A New Species

The development of a new species, Bacillidium vesiculoformis n. sp. (Microspora, Mrazekiidae), is described from the freshwater oligochaete Nais simplex (Oligochaeta, Naididae). Initial stages of parasite development consist of a monokaryotic merogony within a haemocyte of the intestinal blood sinus. The resulting hypertrophied haemocyte is attached to the chloragocytes of the sinus by fine cytoplasmic extensions with the sinus around the cell becoming greatly enlarged. The meronts within the haemocyte form diplokaryotic sporonts that undergo sporogenesis directly within the cytoplasm of the host cell. The infected cell becomes packed with spores and developmental stages, causing it dramatically to increase in size, eventually rupturing the oligochaete and cell. Sporogony appears to be disporoblastic. Released spores were observed to have an adhesive quality. Transmission studies conducted with mature spores failed to transmit the parasite horizontally although vertical transmission was observed. Phylogenetic analysis of the parasite demonstrated that B. vesiculoformis clustered with microsporidian parasites of bryozoa and two other microsporidians, Janacekia debaiseuxi and an unidentified Bacillidium sp.

Description of Diploplastron dehorityi sp. nov. (Entodiniomorphida, Ophryoscolecidae), a new rumen ciliate from Brazilian sheep (Ovis aries)

Zootaxa ◽  
2017 ◽  
Vol 4258 (6) ◽  
pp. 581 ◽  
Author(s):  
FRANCIANE CEDROLA ◽  
ROBERTO JÚNIO PEDROSO DIAS ◽  
ISABEL MARTINELE ◽  
MARTA D’AGOSTO
Keyword(s):  
New Species ◽  
Body Shape ◽  
The Body ◽  
Ovis Aries ◽  
Domestic Sheep ◽  
Diagnostic Features ◽  
Ciliate Community ◽  
The Right ◽  
A New Species

To date the genus Diploplastron comprised only one species of ophryoscolecid ciliate, Diploplastron affine, which is characterized by having two retractable ciliary zones in the anterior end of the body, two slender and juxtaposed skeletal plates on the right side, a rod shape macronucleus, and two contractile vacuoles. During study on the characterization of rumen ciliate community composition in Brazilian domestic sheep, we observed ciliates with atypical morphology but with diagnostic features of genus Diploplastron. This study describes Diploplastron dehorityi, a new species of ophryoscolecid ciliate, that differs from D. affine, primarily, in the morphology of skeletal plates, morphology of nuclear apparatus and body shape. In addition to the similarities between the new species and congener species, D. dehorityi has some morphological similarities to species of genus Eremoplastron.

Morphology and molecular phylogeny of ornamental freshwater prawns of the genus Macrobrachium (Decapoda, Caridea, Palaemonidae) from China with the description of a new species

Crustaceana ◽  
2021 ◽  
Vol 94 (10) ◽  
pp. 1201-1220
Author(s):  
Qinghua Chen ◽  
Wenjian Chen ◽  
Yuanwei Hu ◽  
Ka Yan Ma ◽  
Zhaoliang Guo
Keyword(s):  
New Species ◽  
Colour Pattern ◽  
Molecular Evidence ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Closely Related Species ◽  
Distribution Maps ◽  
Mitochondrial Cytochrome Oxidase ◽  
A New Species

Abstract This study dealt with three species of ornamental palaemonid freshwater prawns of the genus Macrobrachium, based on morphological and molecular analysis. Macrobrachium pentazona He, Gao & Guo, 2009; M. laevis Zheng, Chen & Guo, 2019; and M. bilineare sp. nov. are distinguishable from closely related species by segmental ratios, spination of the second pereiopods and the slender scaphocerite. Macrobrachium bilineare sp. nov. can easily be recognized in the field by its bright colour pattern. Molecular evidence of mitochondrial cytochrome oxidase subunit I (COI), also supports the characterization of this new species, raising the total number of Macrobrachium spp. known from China to 41. Detailed description, illustrations, colour photographs, habitat information, distribution maps and features of conservation significance are also briefly discussed.

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