An Immunonanosilver Surface-Enhnaced Resonance Raman Scattering Method for Determination of Human Chorionic Gonadotrophin

In pH 6.6 Na2HPO4-citric acid buffer solution and in the presence of KCl, the nanosilver-labeled rabbit anti-hCG (Ag-RAhCG) was aggregated un-specifically to the aggregations. Upon addition of rhodamine 6G (RhG) molecular probe, it adsorb on the surface of Ag-RAhCG aggregations that exhibited the strongest surface-enhnaced resonance Raman scattering (SERRS) peak at 613 cm-1. In the optimal condition, the decreased SERRS intensity responds linearly with the concentration of hCG over 0.05-1.75 µg/mL. Based on this, a new and simple SERRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

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A New and Reproducible Surface-Enhanced Resonance Raman Scattering Method for the Determination of Trace Au(III) in Solution Using 4-Mercaptopyridine as Molecular Probe

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.664.32 ◽

2013 ◽

Vol 664 ◽

pp. 32-36

Author(s):

Yan Yan Wei ◽

Gui Qing Wen ◽

Qing Ye Liu ◽

Zhi Liang Jiang

Keyword(s):

Detection Limit ◽

Raman Scattering ◽

Resonance Raman ◽

Molecular Probe ◽

Scattering Method ◽

Resonance Raman Scattering ◽

Buffer Solution ◽

Surface Enhanced ◽

Stable Reagent

In pH 6.5 Na2HPO4-NaH2PO4 buffer solution and in the presence of stable reagent PEG10000 and aggregation reagent NaCl, nanosilvers were aggregated to form stable aggregated-nanosilvers (ANS). The molecular probe of 4-mercaptopyridine (MP) adsorbed on the surface of ANS to form MP-ANS conjugates that produce strongest surface-enhanced resonance Raman scattering (SERRS) peak at 1097 cm-1. When Au(III) was added, the SERRS intensity at 1097 cm-1 decreased. The decreased SERRS intensity was linear to Au(III) concentration in the range of 5-50 ×10-10 mol/L, with a detection limit of 2.0×10-10 mol/L Au(III).

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A Surface-Enhanced Resonance Raman Scattering Method for the Determination of Trace Crystal Violet on Colloidal Au Nanoparticle Substrate

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.299.191 ◽

2013 ◽

Vol 299 ◽

pp. 191-194 ◽

Cited By ~ 1

Author(s):

Zhi Liang Jiang ◽

Lu Ma ◽

Ai Hui Liang

Keyword(s):

Raman Scattering ◽

Crystal Violet ◽

Resonance Raman ◽

Trisodium Citrate ◽

Au Nanoparticle ◽

Scattering Method ◽

Resonance Raman Scattering ◽

Buffer Solution ◽

Surface Enhanced ◽

Colloidal Au

Colloidal Au nanoparticles as active substrate for surface-enhanced resonance Raman scattering(SERRS) were prepared by the trisodium citrate-reduced procedure. In pH 6.6 Na2HPO4-NaH2PO4 buffer solution and in the presence of aggregation reagent NaCl, nanogolds were aggregated to form stable aggregated- nanogolds (ANG). The crystal violet (CV) adsorbed on the surface of ANG to form CV-ANG conjugates that produce strongest surface-enhanced resonance Raman scattering peak at 1616 cm-1. In the optimal condition, the SERRS intensity at 1616 cm-1 was linear to the CV concentration in the range of 2.5×10-8 -1.75×10-7 mol/L, a surface-enhanced resonance Raman scattering assay was set up for detection of trace CV, with good selectivity.

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A Label-Free Nanogold DNAzyme-Cleaved Surface-Enhanced Resonance Raman Scattering Method for Trace UO2 2+ Using Rhodamine 6G as Probe

Plasmonics ◽

10.1007/s11468-012-9476-8 ◽

2012 ◽

Vol 8 (2) ◽

pp. 803-810 ◽

Cited By ~ 18

Author(s):

Zhiliang Jiang ◽

Dongmei Yao ◽

Guiqing Wen ◽

Tingsheng Li ◽

Bin Chen ◽

...

Keyword(s):

Raman Scattering ◽

Rhodamine 6G ◽

Resonance Raman ◽

Scattering Method ◽

Label Free ◽

Resonance Raman Scattering ◽

Surface Enhanced

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A Surface-Enhanced Resonance Raman Scattering Method for Trace Cystine Using 4-Mercaptopyridine-Aggregated-Nanosilver as Probe

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.647.181 ◽

2013 ◽

Vol 647 ◽

pp. 181-184

Author(s):

Yan Yan Wei ◽

Fang Li ◽

Ai Hui Liang ◽

Zhi Liang Jiang

Keyword(s):

Detection Limit ◽

Raman Scattering ◽

Optimal Condition ◽

Resonance Raman ◽

Scattering Method ◽

Resonance Raman Scattering ◽

Buffer Solution ◽

Surface Enhanced ◽

Sers Intensity

A stable nanosilver solution was prepared, using PEG10000 as stabilizer and NaBH4 as reducer. In pH 6.6 Na2HPO4-NaH2PO4 buffer solution containing PEG10000 and NaCl, nanosilvers were aggregated to form the stable aggregated-nanosilvers (ANS) that could conjugate with 4-mercaptopyridine (MP) to produce an ANS-MP surface-enhanced resonance Raman scattering (SERRS) probe, which exhibited a strong SERS peak at 1097 cm-1. When cystine (Cy) concentration increased, the SERS intensity at 1097 cm-1 decreased linearly. In the optimal condition, the decreased SERRS intensity was linear to Cy concentration in the range of 5.0×10-9-1.4×10-7 mol/L, with a detection limit of 2.0×10-9 mol/L Cy.

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Surface-Enhanced Resonance Raman Scattering of Rhodamine 6G in Dispersions and on Films of Confeito-Like Au Nanoparticles

Sensors ◽

10.3390/s17112563 ◽

2017 ◽

Vol 17 (11) ◽

pp. 2563 ◽

Cited By ~ 10

Author(s):

Masaki Ujihara ◽

Nhut Dang ◽

Toyoko Imae

Keyword(s):

Raman Scattering ◽

Au Nanoparticles ◽

Rhodamine 6G ◽

Resonance Raman ◽

Resonance Raman Scattering ◽

Surface Enhanced

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An Immunonanosilver Resonance Rayleigh Scattering Spectral Probe for Rapid Assay of Human Chorionic Gonadotrophin

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.680.141 ◽

2013 ◽

Vol 680 ◽

pp. 141-144 ◽

Cited By ~ 1

Author(s):

Qing Ye Liu ◽

Gui Qing Wen ◽

Kun Li ◽

Ai Hui Liang

Keyword(s):

Citric Acid ◽

Optimal Condition ◽

Rayleigh Scattering ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Resonance Rayleigh Scattering ◽

Serum Samples ◽

Buffer Solution ◽

Citric Acid Buffer

In pH 6.6 Na2HPO4- citric acid buffer solution and in the presence of KCl, the immunoreaction between hCG and nanosilver-labeled anti-hCG took place, the immunonanosilver-complex was formed and deposited, caused the resonance Rayleigh scattering (RRS) intensity at 510 nm decreased. In the optimal condition, the decreased RRS intensity responds linearly with the concentration of hCG over 0.125-1.75 µg/mL. Based on this, a new and simple RRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

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