Preparation of Tetrasubstituted Bis(3-indolyl)methanes from Indoles and Acetophenes Using 1,3-Dibromo-5,5-dimehtylhydantoin as an Efficient Catalyst

A new process that could efficiently prepare tetrasubstituted bis(3-indolyl)methanes from various indoles and acetophenones with 1,3-Dibromo-5,5-dimehtylhydantoin(DBDMH) as a catalyst was reported. The effects of catalysts, solvents, and reaction temperature were investigated. Under the optimal condition, most of the tetrasubstituted bis(3-indolyl)methanes were obtained in 90–99% yields.

IL-17 crosses the blood–brain barrier to trigger neuroinflammation: a novel mechanism in nitroglycerin-induced chronic migraine

Background Chronic migraine places a disabling burden on patients, which is extensively modeled by the nitroglycerin (NTG)-treated animal model. Although the NF-κB pathway is involved in an increase in CGRP levels and activation of the trigeminal system in the NTG model, the relationship between NTG and neuroinflammation remains unclear. This study aimed to optimize a chronic NTG rat model with hyperalgesia and the ethological capacity for estimating migraine therapies and to further explore the underlying mechanism of NTG-induced migraine. Methods Rats were administered different doses of NTG s.c. daily or every 2 d; 30 min and 2 h later, the mechanical threshold was tested. After 9 d, the rats were injected with EB or Cy5.5 for the permeability assay. The other animals were sacrificed, and then, brainstem and caudal trigeminal ganglion were removed to test CGRP, c-Fos and NOS activity; Cytokines levels in the tissue and serum were measured by ELISA; and NF-κB pathway and blood–brain barrier (BBB)-related indicators were analyzed using western blotting. Immunohistochemistry was performed to observe microglial polarization and IL-17A+ T cell migration in the medulla oblongata. Results NTG (10 mg/kg, s.c., every 2 d for a total of 5 injections) was the optimal condition, resulting in progressive hyperalgesia and migraine behavior. TNC neuroinflammation with increases in cytokines, CGRP and c-Fos and activation of the NF-κB pathway was observed, and these changes were alleviated by ibuprofen. Furthermore, NTG administration increased BBB permeability by altering the levels functional proteins (RAGE, LRP1, AQP4 and MFSD2A) and structural proteins (ZO-1, Occludin and VE-cadherin-2) to increase peripheral IL-17A permeation into the medulla oblongata, activating microglia and neuroinflammation, and eventually causing hyperalgesia and migraine attack. Conclusions This study confirmed that NTG (10 mg/kg, s.c., every 2 d for a total of 5 injections) was the optimal condition to provoke migraine, resulting in mechanical hyperalgesia and observable migraine-like behavior. Furthermore, IL-17A crossed the blood–brain barrier into the medulla oblongata, triggering TNC activation through microglia-mediated neuroinflammation. This process was a novel mechanism in NTG-induced chronic migraine, suggesting that IL-17A might be a novel target in the treatment of migraine.

Effect of layer thickness on the overhanging surface quality manufactured by selective laser melting

With the increasing demand for the precision of lightweight part, overhanging surface quality has attracted more and more attentions in recent years. In this work, the relationship between overhanging surface quality and layer thickness was studied. The testing results revealed that with the increasing of the layer thickness, surface quality, especially the surface flatness, showed a significant decreasing trend due to the decreasing of the bending deformation. When layer thickness was ranged from 0.15 to 0.18, it reached a relative optimal condition while the sinking distance and flatness within 0.095 mm–0.1 mm and 0.076 mm–0.078 mm, respectively. With the further increasing of the layer thickness, both sinking distance and surface flatness had a quite significant decreasing due to the increasing gravity of the overhanging layer powder and bonded powder caused by the molten pool sinking. Combined to the experimental data and the analysis given above, the structure of the overhanging surface was optimized and the surface quality showed a further increase.

Optimization Study of Adsorption Parameter for Removal of Dye Pollutant Using Candle Soot Coated Egg Carton

In this study, several parameters that affect the adsorption capacity of Rhodamine B (RhB) dyes were reviewed which include initial pH, temperature, contact time and initial dye concentration. Experimental data was extracted from other literatures as input for subsequent optimization study. Design Expert version 11.1.2.0 software was used to find the optimal condition for adsorption of RhB dyes from wastewater by using candle soot coated on egg carton (CS-Egg) as absorbent. The Central Composite Design (CCD) in response surface methodology (RSM) was employed to perform the optimization and analysis process as it was effective in determining the optimal condition for the adsorption process. CCD evaluated many parameters at the same time and thus reducing the number of experiments required. The optimum condition to maximize the adsorption efficiency was obtained at pH 7, 262.5 mg/L, 60 min of contact time and 55 °C. The highest predicted adsorption efficiency of RhB dye was 90 % at optimum condition. The optimization of parameter provides a better understanding on the adsorption efficiency before conducting the actual experimental work.

The Effect of Pedaling At Different Cadence On Attentional Resources

Research aim: We investigated the relationship between attentional resources and pedaling cadence using electroencephalography (EEG) to measure P300 amplitudes and latencies. Methods: Twenty-five healthy volunteers performed the oddball task while pedaling on a stationary bike or relaxing (no pedaling). We set them four conditions: 1) performing only the oddball task (control), 2) performing the oddball task while pedaling at optimal cadence (optimal), 3) performing the oddball task while pedaling faster than optimal cadence (fast), and 4) performing the oddball task while pedaling slower than optimal cadence (slow). Results: P300 amplitudes at Cz and Pz electrodes under optimal, fast, and slow conditions were significantly lower than that under control conditions. P300 amplitudes at Pz under fast and slow conditions were significantly lower than that under the optimal condition. No significant changes in P300 latency at any electrode were observed under any condition. Conclusion: Our findings revealed that pedaling at non-optimal cadence results in less attention being paid to external stimuli compared with pedaling at optimal cadence.

Mouse Acidic Chitinase Effectively Degrades Random-Type Chitosan to Chitooligosaccharides of Variable Lengths under Stomach and Lung Tissue pH Conditions

Chitooligosaccharides exhibit several biomedical activities, such as inflammation and tumorigenesis reduction in mammals. The mechanism of the chitooligosaccharides’ formation in vivo has been, however, poorly understood. Here we report that mouse acidic chitinase (Chia), which is widely expressed in mouse tissues, can produce chitooligosaccharides from deacetylated chitin (chitosan) at pH levels corresponding to stomach and lung tissues. Chia degraded chitin to produce N-acetyl-d-glucosamine (GlcNAc) dimers. The block-type chitosan (heterogenous deacetylation) is soluble at pH 2.0 (optimal condition for mouse Chia) and was degraded into chitooligosaccharides with various sizes ranging from di- to nonamers. The random-type chitosan (homogenous deacetylation) is soluble in water that enables us to examine its degradation at pH 2.0, 5.0, and 7.0. Incubation of these substrates with Chia resulted in the more efficient production of chitooligosaccharides with more variable sizes was from random-type chitosan than from the block-type form of the molecule. The data presented here indicate that Chia digests chitosan acquired by homogenous deacetylation of chitin in vitro and in vivo. The degradation products may then influence different physiological or pathological processes. Our results also suggest that bioactive chitooligosaccharides can be obtained conveniently using homogenously deacetylated chitosan and Chia for various biomedical applications.

Synthesis and application of nano-MH/MPB as intumescent flame retardant (IFR)

Magnesium hydroxide/melamine phosphate borate (nano MH/MPB), a novel nano-composition intumescent flame retardant, was synthesized with the in-situ reaction method from MgCl2·6H2O sodium hydroxide (NaOH) and melamine phosphate borate (MPB) in the absence of H2O. The structure of the product was confirmed by EDAX IR and XRD. The effects of reaction temperature and time on the dimension of magnesium hydroxide were observed. The effects of mass ratio of magnesium hydroxide to MPB on the flame retardancy of nano-MH/MPB/EP were examined with the limiting oxygen test. The results show that the optimal condition of synthesis of MH/MPB is mMH/mMPB = 0.25, reacting under 75 ℃ for 30 minutes. Finally, the mechanism for flame retardancy of nano-MH/MPB/EP was pilot studied by means of IR of char layer and TG of MH/MPB.

Student’s intentional dimension in interactive learning

The purpose of the article is to identify and substantiate the technologies for using the personal intentions of students and teachers as the basis for the interactivity of learning. At the same time, the mental potential of intention as an orientation and professional choice of students in the educational process is revealed. The essence of intention and interactivity is revealed in the comparative characteristics of the goal, intention, volitional efforts and the desire of students to achieve high results in educational cognition. On the basis of the essential properties of the intention, it was revealed that it is inherent in both the personality of the teacher and the student, so it can be invested in educational tools. An interactive educational environment is used as the optimal condition for revealing the potential of an intention. It has been experimentally substantiated that in the field of academic disciplines that coincide with the intentions of students, sense-creating technologies are more productive, in other disciplines interactive technologies allow expanding the area of intention through the intention of the author, teacher, invested in educational tools. The technologies proposed in the article contribute to the teacher’s productive choice of innovative technologies, depending on the degree of coincidence of students’ personal intentions with the subject area.