An Immunonanosilver Resonance Rayleigh Scattering Spectral Probe for Rapid Assay of Human Chorionic Gonadotrophin

2013 ◽  
Vol 680 ◽  
pp. 141-144 ◽  
Author(s):  
Qing Ye Liu ◽  
Gui Qing Wen ◽  
Kun Li ◽  
Ai Hui Liang
Keyword(s):  
Citric Acid ◽  
Optimal Condition ◽  
Rayleigh Scattering ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Citric Acid Buffer

In pH 6.6 Na2HPO4- citric acid buffer solution and in the presence of KCl, the immunoreaction between hCG and nanosilver-labeled anti-hCG took place, the immunonanosilver-complex was formed and deposited, caused the resonance Rayleigh scattering (RRS) intensity at 510 nm decreased. In the optimal condition, the decreased RRS intensity responds linearly with the concentration of hCG over 0.125-1.75 µg/mL. Based on this, a new and simple RRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

Resonance Rayleigh Scattering Determination of Trace Hemin Basing on Aptamer-Modified Nanogold Catalysis of HAuCl4-Vitamine C

2013 ◽  
Vol 787 ◽  
pp. 400-403
Author(s):  
Jin Chao Dong ◽  
Ai Hui Liang ◽  
Zhi Liang Jiang
Keyword(s):  
Detection Limit ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Strong Resonance ◽  
Scattering Spectra ◽  
Vitamine C ◽  
Nanogold Catalysis

Hemin aptamer was used to modify gold nanoparticles (AuNPs) to obtain a stable aptamer-nanogold probe (AussDNA). In the condition of pH 8.0 Tris-HCl buffer solution containing 50mmol/L NaCl, the substrate chain of AussDNA was cracked by hemin to produce a short single-stranded DNA(ssDNA) and then further combined with hemin to form a stable hemin-ssDNA conjugate. The AuNPs released from AussDNA would be aggregated in the condition of 50mmol/L NaCl and exhibited a strong resonance Rayleigh scattering (RRS) peak at 368nm. Under the selected conditions, the increased RRS intensity (ΔI368nm) was linear to hemin concentration in the range of 5-750nmol/L, with a detection limit of 66 pmol/L. This RRS method was applied to determination of residual hemin in serum samples, with satisfactory results. The remnant AussDNA in the solution exhibited a strong catalytic activity on the gold particle reaction of HAuCl4-vitamine C (VC) that can be monitored by RRS technique at 368 nm. When the hemin concentration increased, the AussDNA decreased, the catalysis decreased, and the RRS intensity at 368nm decreased. The decreased RRS intensity ΔI368nmwas linear to the hemin concentration in the range of 1-200nmol/L, with a detection limit of 54 pmol/L. Accordingly, a sensitivity, selectivity, and simplicity new method of resonance Rayleigh scattering spectra to detect hemin using aptamer-modified nanogold as catalyst was established.

A New Resonance Rayleigh Scattering Assay for the Determination of Trace Hg(II) Using the Immunonanogold as Probe

2013 ◽  
Vol 299 ◽  
pp. 221-224
Author(s):  
Li Li Xu ◽  
Zhi Liang Jiang ◽  
Yu Zhen Wang ◽  
Hong Yang ◽  
An Ping Deng
Keyword(s):  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Carrier Protein ◽  
Spleen Cells ◽  
Buffer Solution ◽  
Myeloma Cells ◽  
Strong Resonance ◽  
Methylmercury Chloride ◽  
Citric Acid Buffer

Nanogold (NG) in size of 10 nm was prepared by the NaBH4 procedure. A new ligand 6-mercaptonicotinic acid (MNA) was used to couple both methylmercury chloride (CH3HgCl) and carrier protein to obtain an immunogen, it was immunized BALB/C mice, and the spleen cells of immunized mice were fused with myeloma cells. The monoclonal antibody (mAb) against mercury (II) ions was produced by the hybridoma technique. The mAb was labeled the NG to prepare an immunonanogold (ING) probe for Hg(II). In pH 5.4 Na2HPO4-citric acid buffer solution and under the condition of ultrasonic irradiation, the ING particles were aggregated un-specifically to form big particles that exhibited a strong resonance Rayleigh scattering (RRS) peak at 580 nm. When the Hg(II) was added, the specific immunoreaction of ING-Hg(II) take place, and the ING-Hg(II) immunocomplex dispersed in the solution that caused the RRS intensity decreasing linearly at 580 nm. The decreased intensity was linear to Hg(II) concentration in the range of 0.025-10 μmol/L, with a detection limit of 1.1 nmol/L Hg(II).

Resonance Rayleigh Scattering for the Determination of Chlorpromazine and Promethazine

2006 ◽  
Vol 59 (12) ◽  
pp. 915 ◽  
Author(s):  
Lanxiang An ◽  
Shaopu Liu ◽  
Zhongfang Liu ◽  
Ling Kong ◽  
Xiaoli Hu
Keyword(s):  
Reaction Mechanism ◽  
Drug Concentration ◽  
Rayleigh Scattering ◽  
Ion Association ◽  
Resonance Rayleigh Scattering ◽  
Optimum Conditions ◽  
Serum Samples ◽  
Spectroscopic Characteristics ◽  
Urine And Serum

Chlorpromazine (CPZ) and promethazine (PZ) can react with potassium ferrioxalate (PF) to form 3:1 ion-association complexes, which can result in a significant enhancement of resonance Rayleigh scattering (RRS) intensity. The maximum scattering peaks are located at 368 nm for CPZ-PF and 370 nm for PZ-PF. The RRS spectroscopic characteristics, the optimum conditions of reactions, and influencing factors have been studied for CPZ-PF and PZ-PF. There is a linear relationship between the RRS intensity and the drug concentration in the range of 0.02–8.00 μg mL–1 for CPZ and 0.04–9.00 μg mL–1 for PZ, and the detection limits (3σ) are 6.6 ng mL–1 for CPZ and 10.6 ng mL–1 for PZ. The proposed method has been applied to determine CPZ in urine and serum samples with satisfactory results. Moreover, the reaction mechanism and the reasons for intensity enhancement of RRS have been discussed.

Resonance Rayleigh Scattering Effect of Immunonanosilver Aggreagations and its Application to Rapid Determination of Trace Hg(II)

2013 ◽  
Vol 664 ◽  
pp. 749-753
Author(s):  
Li Li Xu ◽  
Zhi Liang Jiang ◽  
Ai Hui Liang
Keyword(s):  
Phosphate Buffer ◽  
Rayleigh Scattering ◽  
Sodium Citrate ◽  
Rapid Determination ◽  
Phosphate Buffer Solution ◽  
Resonance Rayleigh Scattering ◽  
Buffer Solution ◽  
Scattering Effect ◽  
Large Particles

Using PEG-10000 and sodium citrate as stabilizer, and NaBH4 as reducer, a stable nanosilvers (AgNPs) sol was prepared. In pH 6.6 phosphate buffer solution containing NaCl, the AgNPs were aggregated to large particles, which lead to resonance Rayleigh scattering (RRS) peak at 350 nm enhancement. Upon addition of cysteine, the peak decreased. The decreased value ΔI is linear to cysteine concentration in the range of 5-60×10-8 mol/L. Thus, a new RRS method was proposed for detection of cysteine.

scholarly journals Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Liping Wu ◽  
Yue Liu ◽  
Rong Huang ◽  
Huawen Zhao ◽  
Weiqun Shu
Keyword(s):  
Rayleigh Scattering ◽  
Folate Receptor ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Au Nps ◽  
Selective Determination ◽  
Limit Of Determination

A rapid, simple, and novel method for folate receptor α (FRα) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FRα antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FRα was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (ΔIRRS) and the concentration of FRα, with the detecting range of 0.50–37.50 ng·mL−1 and the limit of determination of 0.05 ng·mL−1. The determination of FRα in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.

A Resonance Rayleigh Scattering Method for the Determination of Trace Benzotriazole Based on Complex Particle

2013 ◽  
Vol 830 ◽  
pp. 448-451
Author(s):  
Ling Ling Ye ◽  
Ai Hui Liang
Keyword(s):  
Detection Limit ◽  
Nitrogen Atom ◽  
Rayleigh Scattering ◽  
Hydroxylamine Hydrochloride ◽  
Regression Equation ◽  
Resonance Rayleigh Scattering ◽  
Scattering Method ◽  
Buffer Solution ◽  
Complex Particle

In pH 4.2 HAc-NaAc buffer solution, hydroxylamine hydrochloride reduced Cu2+ to Cu+ that coordinate the nitrogen atom of 1,2,3-benzotriazole (BTA) to form Cu-BTA complex particles with a resonance Rayleigh scattering (RRS) peak at 369 nm. Under the selected conditions, when the BTA concentration increased, the RRS intensity at 369 nm increased. The increased RRS intensity ΔI369nm was linear to BTA concentration in the range of 0.17-13.36 µg/mL, with a regression equation of ΔI369nm = 89.91C + 96.7, and the detection limit is 0.17 µg/mL. Accordingly, a new RRS method for BTA was established.

A novel ternary system for the determination of ascorbic acid concentration based on resonance Rayleigh scattering

2015 ◽  
Vol 7 (23) ◽  
pp. 9963-9970 ◽  
Author(s):  
Qin Li ◽  
Zhan Zhang ◽  
Wenyan Yao ◽  
Jin Li ◽  
Jidong Yang
Keyword(s):  
Ascorbic Acid ◽  
Ternary System ◽  
Acid Concentration ◽  
Rayleigh Scattering ◽  
Reducing Power ◽  
Resonance Rayleigh Scattering ◽  
Ascorbic Acid Concentration ◽  
Buffer Solution

The dienol of ascorbic acid was observed to have a strong reducing power in a 1.44 M HCl buffer solution, and could reduce Fe3+ to Fe2+.

An Immunonanosilver Surface-Enhnaced Resonance Raman Scattering Method for Determination of Human Chorionic Gonadotrophin

2013 ◽  
Vol 319 ◽  
pp. 157-160 ◽  
Author(s):  
Xin Hui Zhang ◽  
Kun Li ◽  
Le Ning Hu ◽  
Ai Hui Liang ◽  
Zhi Liang Jiang
Keyword(s):  
Raman Scattering ◽  
Rhodamine 6G ◽  
Resonance Raman ◽  
Molecular Probe ◽  
Scattering Method ◽  
Resonance Raman Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Citric Acid Buffer

In pH 6.6 Na2HPO4-citric acid buffer solution and in the presence of KCl, the nanosilver-labeled rabbit anti-hCG (Ag-RAhCG) was aggregated un-specifically to the aggregations. Upon addition of rhodamine 6G (RhG) molecular probe, it adsorb on the surface of Ag-RAhCG aggregations that exhibited the strongest surface-enhnaced resonance Raman scattering (SERRS) peak at 613 cm-1. In the optimal condition, the decreased SERRS intensity responds linearly with the concentration of hCG over 0.05-1.75 µg/mL. Based on this, a new and simple SERRS method has been proposed for the determination of hCG in serum samples, with satisfactory results.

Nanogold Resonance Rayleigh Scattering Determination of Trace Cr3+

2013 ◽  
Vol 788 ◽  
pp. 19-22
Author(s):  
Xiao Jing Liang ◽  
Ai Hui Liang
Keyword(s):  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Buffer Solution ◽  
Binding Stoichiometry ◽  
Citrate Complex ◽  
Citrate Reduction

Nanogolds (AuNPs) were synthesized with the citrate reduction of HAuCl4. In pH 3.0 glycineHCl buffer solution (0.20 mol/L), AuNPs do not aggregate. In the presence of Cr (III), that Cr (III) could form astable Cr (III) citrate complex with the citrate on surface of AuNP in 1: 2 binding stoichiometry, and the AuNPs were aggregated to big AuNPs clusters that led to the resonance Rayleigh scattering (RRS) peak at 530 nm increased greatly. Under the selected conditions, the increased RRS intensity (ΔI530nm) is linear to Cr (III) concentration in the range of 0.25-5.0 μmol/L. This RRS method was applied to determination of Cr (III) in synthetic samples, with satisfactory results. Cr (VI) was also detected after reduction to Cr (III).

A Nanosilver Resonance Rayleigh Scattering Method for the Determination of Trace Cysteine

2013 ◽  
Vol 664 ◽  
pp. 741-745
Author(s):  
Xin Hui Zhang ◽  
Yan Yan Wei ◽  
Chi Zhang ◽  
Miao Miao Chi ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Rayleigh Scattering ◽  
Sodium Citrate ◽  
Phosphate Buffer Solution ◽  
Resonance Rayleigh Scattering ◽  
Scattering Method ◽  
Buffer Solution ◽  
Large Particles

Using PEG-10000 and sodium citrate as stabilizer, and NaBH4 as reducer, a stable nanosilver (AgNPs) sol was prepared. In pH 6.6 phosphate buffer solution containing NaCl, the AgNPs were aggregated to large particles, which lead to resonance Rayleigh scattering (RRS) peak at 350 nm enhancement. Upon addition of cysteine, the peak decreased. The decreased value ΔI is linear to cysteine concentration in the range of 5.0×10-8-6.0×10-7 mol/L. Thus, a new RRS method was proposed for detection of cysteine.

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