Construction of Eukaryotic Expression Vector and Expression on Mycobacterium bovis ag85a and mpb70 Genes

2013 ◽  
Vol 421 ◽  
pp. 308-312
Author(s):  
Shuang Hou ◽  
Chun Fang Wang ◽  
Yan Ru Zheng ◽  
Jia Ning Guan ◽  
Jia Ming Lin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Bovis ◽  
Recombinant Plasmid ◽  
Expression Vector ◽  
Fusion Gene ◽  
Eukaryotic Expression ◽  
Chain Reaction ◽  
Eukaryotic Expression Vector ◽  
Polymerase Chain

Based on the polymerase chain reaction (PCR),ag85aandmpb70Fusion gene ofMycobacterium boviswere ligated and cloned into pMD18-T, then recombinant plasmid pMD-85a-70 was constructed. pMD-85a-70 and pVAX1-BMS were digested by double enzymesHindIII andEcoRI, the purified ag85a-mpb70 was subcloned into pVAX1-BMS, then recombinant plasmid pVAX1-BMS-85a-70 was constructed and transient expressed in Marc145 cell. These results laid solid foundations for further studies on ag85a-mpb70.

Construction of Eukaryotic Expression Vector and Transient Expression on Mycobacterium bovis mpb83,mpb70 and Cattle il-15 Gene

2013 ◽  
Vol 749 ◽  
pp. 177-181
Author(s):  
Yan Ru Zheng ◽  
Chun Fang Wang ◽  
Yun Hang Gao ◽  
Jia Ning Guan ◽  
Jia Ming Lin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Bovis ◽  
Recombinant Plasmid ◽  
Expression Vector ◽  
Fusion Gene ◽  
Eukaryotic Expression ◽  
Chain Reaction ◽  
Eukaryotic Expression Vector ◽  
Polymerase Chain ◽  
I Gene

Based on the splicing by overlapping extension (SOE) polymerase chain reaction (PCR),fusion gene mpb83-70 andil-15were ligated and cloned into pMD18-T,then recombinant plasmid pMD-83-70-15 was constructed. pMD-83-70-15 and pVAX1-BMS were digested by double enzymesHindIII andEcoRI, the purified mpb83-70-il-15 was subcloned into pVAX1-BMS,then recombinant plasmid pVAX1-BMS-83-70-15 was constructed and transient expressed in Mark145 cell. These results laid solid foundations for further studies on mpb83-70-il-15.

Construction and Expression of a Eukaryotic Expression Vector Containing a Fusion Gene of the Hantaan Virus S Gene and Hsp70 Gene

2008 ◽  
Vol 58 (1) ◽  
pp. 30-34
Author(s):  
Juan Gao ◽  
Bicheng Zhang ◽  
Shoujing Yang ◽  
Kainan Li ◽  
Hualin Xu ◽  
...  
Keyword(s):  
Expression Vector ◽  
Fusion Gene ◽  
Eukaryotic Expression ◽  
Hantaan Virus ◽  
Hsp70 Gene ◽  
Eukaryotic Expression Vector ◽  
S Gene

scholarly journals Use of Touch-Down Polymerase Chain Reaction to Enhance the Sensitivity of Mycobacterium Bovis Detection

2005 ◽  
Vol 17 (3) ◽  
pp. 232-238 ◽  
Author(s):  
Martín J. Zumárraga ◽  
Virginia Meikle ◽  
Amelia Bernardelli ◽  
Alejandro Abdala ◽  
Hector Tarabla ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Bovis ◽  
Pcr Amplification ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Single Tube ◽  
Nasal Swabs ◽  
Diagnostic Samples ◽  
Polymerase Chain ◽  
Duplication Time

The confirmatory diagnosis of Mycobacterium bovis ( M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS 6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.

scholarly journals Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis

2016 ◽  
Vol 78 (12) ◽  
pp. 1873-1876 ◽  
Author(s):  
Zhang QUAN ◽  
Tan HAIMING ◽  
Cai XIAOYAO ◽  
Yuan WEIFENG ◽  
Jia HONG ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycobacterium Bovis ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of AML1/MTG8 fusion gene of spinal granulocytic sarcoma in a nonleukemic patient using the real-time polymerase chain reaction

2005 ◽  
Vol 10 (4) ◽  
pp. 414-417
Author(s):  
Shojiro Nakashima ◽  
Kazuhiro Suzuki ◽  
Ken Watanabe ◽  
Shiro Urata ◽  
Shuichi Mizuta ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Fusion Gene ◽  
Granulocytic Sarcoma ◽  
Chain Reaction ◽  
The Real ◽  
Polymerase Chain
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