A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on ITS Sequences

2014 ◽  
Vol 1033-1034 ◽  
pp. 179-182
Author(s):  
Shao Xuan Zhang ◽  
Guang Zhu Lin ◽  
Bo Chuan Wang ◽  
Yan Shi
Keyword(s):  
Its Region ◽  
Jilin Province ◽  
Its Sequences ◽  
New Method ◽  
Universal Primers ◽  
Reliable Identification ◽  
Identification Method ◽  
Restriction Sites ◽  
Pcr Products

To establish a reliable identification method of Pulsatilla chinesis (Bge) Regel., from its counterfeits, Potentilla chinesis Ser., we extracted the genome DNA from the leaves of these two plants collected in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and compared. Based on ITS sequences, we used primer PREMIER 5.0 to search the restriction sites of the two ITS sequences and found BtgI can be used to identify them.

A New Method to Identify Pulsatilla chinesis(Bge) Regel, from Potentilla chinesis Ser. Based on Chloroplast trnL-F Sequences

2014 ◽  
Vol 1033-1034 ◽  
pp. 175-178
Author(s):  
Shao Xuan Zhang ◽  
Guang Zhu Lin ◽  
Bo Chuan Wang ◽  
Yan Shi
Keyword(s):  
Jilin Province ◽  
New Method ◽  
Universal Primers ◽  
Reliable Identification ◽  
Identification Method ◽  
F Region ◽  
Restriction Sites ◽  
Pcr Products

To establish a reliable identification method of Pulsatilla chinesis (Bge) Regel., from its counterfeits, Potentilla chinesis Ser., we extracted the genome DNA from the leaves of these two plants collected in Jilin Province, amplified the chloroplast trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and compared. Based on trnL-F sequences, we used primer PREMIER 5.0 to search the restriction sites of the two trnL-F sequences and found RsaI can be used to identify them.

Comparison of the ITS Sequences of 5 Common Potentilla Species in Jilin Province of China

2012 ◽  
Vol 554-556 ◽  
pp. 1690-1693 ◽  
Author(s):  
Shao Xuan Zhang ◽  
Xin Rui Liu ◽  
Bo Chuan Wang ◽  
Yun Hui Ling ◽  
De Jun Sun ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Its Region ◽  
Scientific Data ◽  
Jilin Province ◽  
Its Sequences ◽  
Universal Primers ◽  
Its Sequence ◽  
Its Sequence Analysis ◽  
Pcr Products

To find the differences in the internal transcribed spacer(ITS) sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. Polymorphism of ITS sequences was found within P. chinensis and the sequence data suggested that our samples of this species might be related to hybridization. Other 4 species showed intraspecies-stability in ITS sequence. The ITS sequences of these 5 Potentilla species are significantly different. So ITS sequence analysis and other methods derived from it can be used in authentication of Potentilla.

ITS Sequences Analysis of Pulsatilla koreana Nakai. and Potentilla chinesis Ser.

2013 ◽  
Vol 749 ◽  
pp. 246-249
Author(s):  
Shao Xuan Zhang ◽  
Feng Liu ◽  
Yun Hui Ling ◽  
Bo Chuan Wang ◽  
De Jun Sun ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Its Region ◽  
Scientific Data ◽  
Jilin Province ◽  
Its Sequences ◽  
Universal Primers ◽  
Pcr Products ◽  
Sequences Analysis

To provide scientific data of the internal transcribed spacer (ITS) sequences for the authentication of Pulsatilla chinesis (Bge) Regel, we extracted the genome DNA from the leaves of Pulsatilla koreana Nakai. and Potetilla chinesis Ser. collected in Jilin Province, amplified the ITS region using ITS universal primers of angiosperm, and sequenced the purified PCR products directly. Polymorphism of ITS sequences was found within P. chinensis Ser. and the sequence data suggested that our samples of this species might be related to hybridization. The obtained sequences were edited by Genetyx and reported here.

Comparison of Chloroplast TrnL-F Sequences of 5 Common Potentilla Species in Jilin Province of China

2012 ◽  
Vol 554-556 ◽  
pp. 1730-1733 ◽  
Author(s):  
Shao Xuan Zhang ◽  
Xin Rui Liu ◽  
Yun Hui Ling ◽  
Bo Chuan Wang ◽  
De Jun Sun ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Related Species ◽  
Scientific Data ◽  
Jilin Province ◽  
Universal Primers ◽  
F Region ◽  
Pcr Products

To find the differences in the trnL-F sequences and provide scientific data for the authentication of Potentilla chinensis and its related species, we extracted the genome DNA from the leaves of 5 common Potetilla species in Jilin Province, amplified the trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. We found that the trnL-F sequences of different species of Potentilla are significantly different. So trn-L sequence analysis and other methods derived from it can be used in authentication of Potentilla.

scholarly journals rDNA Variability Assessed in PCR Reactions of Selected Accessions of Acer

2011 ◽  
Vol 39 (1) ◽  
pp. 260 ◽  
Author(s):  
Milosz SMOLIK ◽  
Marcelina KRUPA-MAŁKIEWICZ ◽  
Beata SMOLIK ◽  
Justyna WIECZOREK ◽  
Katarzyna PREDYGIER
Keyword(s):  
Genetic Similarity ◽  
Its Region ◽  
Universal Primers ◽  
Its Sequence ◽  
Genotypic Variability ◽  
Botanical Variety ◽  
Rdna Sequences ◽  
Pcr Products ◽  
Genetic Profiles ◽  
Rdna Variability

The region of rDNA (SSU, 5.8S, LSU and ITS1, ITS2 spacer sequences) of fourteen Acer accessions was tested in order to determine the possibility to identify genotypic variability within a rDNA sequence. The tests were performed using the PCR technique and a set of combination of several pairs of ‘universal’ primers designed to amplify rDNA sequences. ‘Universal’ primers were selected in such a way as to make the rDNA regions flanking possible in cases where a smaller or larger scope of variability was expected. Thus, within SSU or LSU sequences, monomorphic PCR products were amplified for the tested Acer accessions. Within the ITS sequence, a greater scope of variability was found. This was manifested by the presence of several additional PCR products in the genetic profiles of the tested Acer accessions. The range of their length relative to the ITS region or ITS1, ITS2 or 5.8S corresponded separately to that described in the literature. The analysis of the topology of the constructed tree revealed the presence of two similar groups, ‘a’ and ‘b’. Cultivated varieties and one botanical variety of the Japanese maple were included respectively in these groups and the genetic similarity between the analyzed accessions ranged from 63 to 98%.

Chloroplast TrnL-F Sequences Analysis of Pulsatilla koreana Nakai. and Potentilla chinesis Ser.

2013 ◽  
Vol 749 ◽  
pp. 242-245
Author(s):  
Shao Xuan Zhang ◽  
Feng Liu ◽  
Bo Chuan Wang ◽  
Yun Hui Ling ◽  
De Jun Sun ◽  
...  
Keyword(s):  
Scientific Data ◽  
Jilin Province ◽  
Universal Primers ◽  
F Region ◽  
Pcr Products ◽  
Sequences Analysis

To provide scientific data of the chloroplast TrnL-F sequences for the authentication of Pulsatilla chinesis (Bge) Regel, we extracted the genome DNA from the leaves of Pulsatilla koreana Nakai. and Potetilla chinesis Ser. collected in Jilin Province, amplified the chloroplast TrnL-F region using universal primers and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.

scholarly journals A New Method for Rapid Screening of End-Point PCR Products: Application to Single Genome Amplified HIV and SIV Envelope Amplicons

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128188 ◽  
Author(s):  
Laurent Houzet ◽  
Claire Deleage ◽  
Anne-Pascale Satie ◽  
Laetitia Merlande ◽  
Dominique Mahe ◽  
...  
Keyword(s):  
New Method ◽  
Rapid Screening ◽  
End Point ◽  
Single Genome ◽  
Pcr Products

scholarly journals Rapid Detection of Phytophthora infestans in Late Blight-Infected Potato and Tomato Using PCR

Plant Disease ◽  
1997 ◽  
Vol 81 (9) ◽  
pp. 1042-1048 ◽  
Author(s):  
C. L. Trout ◽  
J. B. Ristaino ◽  
M. Madritch ◽  
T. Wangsomboondee
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Pcr Amplification ◽  
Accurate Method ◽  
Phytophthora Species ◽  
Universal Primers ◽  
Direct Pcr ◽  
Field Samples ◽  
Pcr Products ◽  
Oomycete Pathogen

Late blight caused by the oomycete pathogen Phytophthora infestans is a devastating disease of potato and tomato worldwide. A rapid and accurate method for specific detection of P. infestans is necessary for determination of late blight in infected fruit, leaves, and tubers. Ribosomal DNA (rDNA) from four isolates of P. infestans representing the four genotypes US1, US6, US7, and US8 was amplified using polymerase chain reaction (PCR) and the universal primers internal transcribed spacer (ITS) 4 and ITS5. PCR products were sequenced using an automated sequencer. Sequences were aligned with published sequences from 5 other Phytophthora species, and a region specific to P. infestans was used to construct a PCR primer (PINF). Over 140 isolates representing 14 species of Phytophthora and at least 13 other genera of fungi and bacteria were used to screen the PINF primer. PCR amplification with primers PINF and ITS5 results in amplification of an approximately 600 base pair product with only isolates of P. infestans from potato and tomato, as well as isolates of P. mirabilis and P. cactorum. P. mirabilis and P. cactorum are not pathogens of potato; however, P. cactorum is a pathogen of tomato. P. infestans and P. cactorum were differentiated by restriction digests of the amplified product. The PINF primer was used with a rapid NaOH lysis technique for direct PCR of P. infestans from infected tomato and potato field samples. The PINF primer will provide a valuable tool for detection of P. infestans in potatoes and tomatoes.

scholarly journals First Report of a Group 16SrII Phytoplasma Infecting Chickpea in Oman

Plant Disease ◽  
2006 ◽  
Vol 90 (7) ◽  
pp. 973-973 ◽  
Author(s):  
N. A. Al-Saady ◽  
A. M. Al-Subhi ◽  
A. Al-Nabhani ◽  
A. J. Khan
Keyword(s):  
Nested Pcr ◽  
Animal Feed ◽  
Restriction Enzymes ◽  
Universal Primers ◽  
Polymorphism Analysis ◽  
Disease Symptoms ◽  
First Report ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Group 2

Chickpea (Cicer arietinum), locally known as “Dungo”, is grown for legume and animal feed mainly in the interior region of Oman. During February 2006, survey samples of chickpea leaves from plants showing yellows disease symptoms that included phyllody and little leaf were collected from the Nizwa Region (175 km south of Muscat). Total nucleic acid was extracted from asymptomatic and symptomatic chickpea leaves using a cetyltrimethylammoniumbromide method with modifications (3). All leaf samples from eight symptomatic plants consistently tested positive using a polymerase chain reaction assay (PCR) with phytoplasma universal primers (P1/P7) that amplify a 1.8-kb phytoplasma rDNA product and followed by nested PCR with R16F2n/R16R2 primers yielding a product of 1.2 kb (2). No PCR products were evident when DNA extracted from healthy plants was used as template. Restriction fragment length polymorphism analysis of nested PCR products by separate digestion with Tru9I, HaeIII, HpaII, AluI, TaqI, HhaI, and RsaI restriction enzymes revealed that a phytoplasma belonging to group 16SrII peanut witches'-broom group (2) was associated with chickpea phyllody and little leaf disease in Oman. Restriction profiles of chickpea phytoplasma were identical with those of alfalfa witches'-broom phytoplasma, a known subgroup 16SrII-B strain (3). To our knowledge, this is the first report of phytoplasma infecting chickpea crops in Oman. References: (1) A. J. Khan et al. Phytopathology, 92:1038, 2002. (2). I.-M. Lee et al. Int. J. Syst. Bacteriol. 48:1153, 1998 (3) M. A. Saghai-Maroof et al. Proc. Natl. Acad. Sci. USA. 81:8014, 1984.

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