Novel PAHs-Degrading Strain Yarrowia lipolytica TZYx3 and its Synergistic Interaction with HA

2015 ◽  
Vol 1092-1093 ◽  
pp. 1064-1067
Author(s):  
Xin Xin Zhang ◽  
Hong Yan Yu ◽  
Yu Xin Wang ◽  
Han Gui Wu
Keyword(s):  
Yarrowia Lipolytica ◽  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Degradation Rate ◽  
Mineral Oil ◽  
Synergistic Interaction ◽  
Strain Identification ◽  
Polycyclic Aromatic ◽  
Soil Strain ◽  
Identification And Characterization

A novel PAHs (polycyclic aromatic hydrocarbons)-degrading strain, TAYx3 was isolated from mineral oil and heavy metal contaminated soil. Strain identification and characterization showed that TAYx3 wasYarrowia lipolytica.Tests forYarrowia lipolyticaTAYx3 growth on different substances are also described. The strain could use all PAHs tasted as sole carbon source, and showed synergistic interaction with humic acid (HA). With the existence of HA, the degradation rate of TAYx3 was naphthalene 90.7%, phenanthrene91.0%, fluoranthene74.7%, pyrene 86.9%, benzanthracene 84.7%, benzopyrene 74.7%, respectively.

scholarly journals Characterization of the Phenanthrene-Degrading Sphingobium yanoikuyae SJTF8 in Heavy Metal Co-Existing Liquid Medium and Analysis of Its Metabolic Pathway

2020 ◽  
Vol 8 (6) ◽  
pp. 946 ◽  
Author(s):  
Chong Yin ◽  
Weiliang Xiong ◽  
Hua Qiu ◽  
Wanli Peng ◽  
Zixin Deng ◽  
...  
Keyword(s):  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Genome Mining ◽  
Whole Genome Sequence ◽  
Phenanthrene Degradation ◽  
Pah Degradation ◽  
Polycyclic Aromatic ◽  
Degradation Ability ◽  
Halogenated Aromatic Compounds

Polycyclic aromatic hydrocarbons (PAHs) are common organic pollutants with great carcinogenic threaten, and metal/PAH-contaminated environments represent one of the most difficult remedial challenges. In this work, Sphingobium yanoikuyae SJTF8 was isolated and identified with great and stable PAH-degrading efficiency even under stress conditions. It could utilize typical PAHs (naphthalene, phenanthrene, and anthracene) and heterocyclic and halogenated aromatic compounds (dibenzothiophene and 9-bromophenanthrene) as the sole carbon source. It could degrade over 98% of 500 mg/L phenanthrene in 4 days, and the cis-3,4-dihydrophenanthrene-3,4-diol was the first-step intermediate. Notably, strain SJTF8 showed great tolerance to heavy metals and acidic pH. Supplements of 0.30 mM of Cu2+, 1.15 mM of Zn2+, and 0.01 mM of Cd2+ had little effect on its cell growth and phenanthrene degradation; phenanthrene of 250 mg/L could still be degraded completely in 48 h. Further, the whole genome sequence of S. yanoikuyae SJTF8 was obtained, and three plasmids were found. The potential genes participating in stress-tolerance and PAH-degradation were annotated and were found mostly distributed in plasmids 1 and 2. Elimination of plasmid 2 resulted in the loss of the PAH-degradation ability. On the basis of genome mining results, the possible degrading pathway and the metabolites of S. yanoikuyae SJTF8 to phenanthrene were predicted.

Biodegradation of polycyclic aromatic hydrocarbons (PAHs) by microbial consortia enriched from mangrove sediments

2003 ◽  
Vol 48 (8) ◽  
pp. 177-183 ◽  
Author(s):  
N.F.Y. Tam ◽  
C.L. Guo ◽  
C. Yau ◽  
L. Ke ◽  
Y.S. Wong
Keyword(s):  
Aromatic Hydrocarbons ◽  
Sole Carbon Source ◽  
Surface Sediments ◽  
Microbial Consortium ◽  
The Other ◽  
Microbial Consortia ◽  
Mangrove Sediments ◽  
Mangrove Swamp ◽  
Polycyclic Aromatic ◽  
Enriched Cultures

Surface sediments from two mangrove swamps in Hong Kong were enriched to obtain PAH-degrading microbial consortia. The consortium from sediments enriched with phenanthrene (Phe) in Ma Wan, a mangrove swamp next to a boat anchorage, was effective in degrading PAH with 96.7% and 89.9% Phe degradation at 0 and 10 ppt salinities, respectively. The Phe-enriched microbial consortium could also use pyrene (Pyr) as the sole carbon source but the degradation was much less (around 15%). On the other hand, the Phe-enriched consortium from sediments in Yi O, a mangrove swamp recently polluted by an oil spill, completely removed Phe and Pyr at both 0 and 10 ppt salinities. This suggests that different consortia were selected in two sediments using the same PAH substrate. Not only sediments, PAH substrate used for enrichment also selected different consortia. The Pyr- and Fla-enriched Yi O consortia only achieved 10.5 and 4.5% Phe degradation, respectively and the corresponding pyrene degradation was 7 and 40%. Among the three PAH compounds, fluoranthene (Fla) was most difficult to degrade, and 21.1, 11.3, and 36.8% Fla were degraded by Phe-, Fla- and Pyr-enriched cultures, respectively.

scholarly journals Degradation of Polycyclic Aromatic Hydrocarbons by a Newly Discovered Enteric Bacterium, Leclercia adecarboxylata

2004 ◽  
Vol 70 (5) ◽  
pp. 3163-3166 ◽  
Author(s):  
Priyangshu Manab Sarma ◽  
Dhruva Bhattacharya ◽  
S. Krishnan ◽  
Banwari Lal
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Molecular Typing ◽  
Sole Carbon Source ◽  
Clinical Strain ◽  
Oily Sludge ◽  
Rrna Gene ◽  
Enteric Bacterium ◽  
Polycyclic Aromatic ◽  
The 16S Rrna Gene

ABSTRACT A bacterial strain, PS4040, capable of degrading polycyclic aromatic hydrocarbons for use as the sole carbon source was isolated from oily-sludge-contaminated soil. The 16S rRNA gene showed 98.8% homology to that of Leclercia adecarboxylata. Comparative molecular typing with the clinical strain of L. adecarboxylata revealed that there were few comigrating and few distinct amplimers among them.

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