Influence on Surface Characteristics of Microalgae Cell by Solution Chemistry

2011 ◽  
Vol 287-290 ◽  
pp. 1938-1942 ◽  
Author(s):  
Hai Yang Zhang ◽  
Ya Li Kuang ◽  
Zhe Lin ◽  
Chun Hua Liu

Considering the influence on the downstream process of microalgae recovery by cell surface characteristics, in this paper , Microelectrophoresis and Hydrocarbon adsorption have been used for researching the surface electrical property and hydrophobicity of microalgae. The influence on surface characteristics of microalgae cell have been studied by the solution chemistry condition of pH and cationic flocculant. The results show that: The surface charge of microalgae cell is negative under the natural condition. The surface electrical property decreases with the declining pH when pH is less than 7 , but the isoelectric point is not observed until pH low to 1, while it changes little with the pH increased when pH is more than 7; Within the value of experimental pH, microalgae cells have strong hydrophobicity, which increases with the decreasing pH when pH is less than 7 and rises with the pH increased when pH is more than 9. It attains to the minimum value when the pH is about 8. The hydrophobicity of microalgae increases with anhydrous ferric chloride(FeCl3) when cationic flocculant (FeCl3) is added , but the trend became weaker. So the hydrophobicity can be improved by cationic flocculant, which can promote the microalgae cohesion.

2020 ◽  
Vol 55 (5) ◽  
pp. 713-723
Author(s):  
Zambaga Khorolsuren ◽  
Orsolya Lang ◽  
Eva Pallinger ◽  
Anna Foldes ◽  
Gyulai‐Gaál Szabolcs ◽  
...  

2000 ◽  
Vol 182 (6) ◽  
pp. 1706-1713 ◽  
Author(s):  
Mark A. Bittinger ◽  
Jo Handelsman

ABSTRACT RosR is a determinant of nodulation competitiveness and cell surface characteristics of Rhizobium etli and has sequence similarity to a family of transcriptional repressors. To understand how RosR affects these phenotypes, we mutagenized a rosR mutant derivative of R. etli strain CE3 with a mini-Tn5 that contains a promoterless gusA gene at one end, which acts as a transcriptional reporter. Using a mass-mating technique, we introduced rosR into each mutant in trans and screened for mutants that expressed different levels of β-glucuronidase activity in the presence and absence ofrosR. A screen of 18,000 mutants identified 52 insertions in genes negatively regulated by RosR and 1 insertion in a gene positively regulated by RosR. Nucleotide sequence analysis of the regions flanking the insertions suggests that RosR regulates genes of diverse function, including those involved in polysaccharide production and in carbohydrate metabolism and those in a region containing sequence similarity to virC1 and virD3 fromAgrobacterium tumefaciens. Two of the mutants produced colonies with altered morphology and were more competitive in nodulation than was CE3ΔrosR, the rosRparent. One mutant that contained an insertion in a gene with similarity to exsH of Sinorhizobium melilotidid not nodulate the plant host Phaseolus vulgaris withoutrosR. These results indicate that RosR directly or indirectly influences expression of diverse genes in R. etli, some of which affect the cell surface and nodulation competitiveness.


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