Isolation of ACC Deaminase-Containing Plant Growth-Promoting Rhizobacteria from Porrum Rhizasphere Soil

2011 ◽  
Vol 356-360 ◽  
pp. 240-243
Author(s):  
Chang Hong Guo ◽  
Xu Han Shi ◽  
Jia Li Liu
Keyword(s):  
Plant Growth ◽  
Rhizosphere Soil ◽  
Acc Deaminase ◽  
Nitrogen Sources ◽  
Plant Growth Promoting Rhizobacteria ◽  
16S Rdna Sequence ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
High Level ◽  
Chinese Chives

A bacterial strain 3-1 which could use ACC as the sole nitrogen sources was isolated from Chinese chives rhizosphere soil. The strain was identified as Pseudomonas fluorescent, according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. The ACC deaminase activity of Pseudomonas fluorescent 3-1 strain had high level of ACC deaminase activity, 2.63±0.12 µmol α-KA • (mg Pr •h)-1 . The procedure described here was relatively straight forward to isolate and characterize new PGPR strains. This bacterium may be used to facilitate the growth of Porrum.

Isolation of ACC Deaminase-Containing Plant Growth-Promoting Rhizobacteria from Petroleum Contaminated Soil

2011 ◽  
Vol 356-360 ◽  
pp. 244-247
Author(s):  
Chang Hong Guo ◽  
Fang Fang ◽  
Jia Li Liu
Keyword(s):  
Economic Development ◽  
Plant Growth ◽  
Contaminated Soil ◽  
Acc Deaminase ◽  
Nitrogen Sources ◽  
Plant Growth Promoting Rhizobacteria ◽  
16S Rdna Sequence ◽  
Plant Growth Promoting ◽  
Petroleum Contaminated Soil ◽  
Growth Promoting

Petroleum is the most important energy for economic development in China and other countries. But in the process of mining, storage and producing, it has caused many aspects of pollution. In this study, a bacterial strain A-3 which could use ACC as the sole nitrogen sources was isolated from petroleum-contaminated soil. The strain was identified as Achromobacter, according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. Strain Achromobacter A-3 could produce ACC deaminase, and could be used for remediation of petroleum-contaminated areas in future.

scholarly journals Characterization and optimization of 1-Aminocyclopropane-1-Carboxylate Deaminase (ACCD) activity in different rhizospheric PGPR along with Microbacterium sp. strain ECI-12A

2013 ◽  
Vol 1 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Umesh P. Shrivastava ◽  
Ashok Kumar
Keyword(s):  
Plant Growth ◽  
Pcr Amplification ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Optimum Activity ◽  
Rice Rhizosphere ◽  
Acds Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Culture Growth

A total of nine strains of plant growth promoting rhizobacteria were analyzed for ACC deaminase activity, where highest ACC deaminase activity was found in Klebsiella sp strain ECI-10A (539.1 nmol α-keto butyrate/ mg protein/ h) and lowest in Microbacterium sp strain ECI-12A (122.0 nmol α-keto butyrate/ mg protein/ h). Although Microbacterium sp strain ECI-12A showed lowest level of ACC deaminase activity, but, the species of Microbacterium isolated from rhizosphere is the first report. Microbacterium sp strain ECI-12A was also analyzed under varying conditions of time, amount of 1-Aminocyclopropane-1- carboxylate (ACC), and temperature for optimization of the ACC deaminase activity. The optimum activity was recorded with the supplementation of 5mM ACC at 30°C temperature after 24h of culture growth. All the nine strains showed acdS gene in the PCR amplification of that gene. No any rhizospheric Microbacterium species showing ACC deaminase activity have been reported earlier, therefore, we report here ACC deaminase activity in Microbacterium sp ECI-12A isolated from rice rhizosphere is a novel finding. DOI: http://dx.doi.org/10.3126/ijasbt.v1i1.7921 Int J Appl Sci Biotechnol, 2013, Vol. 1(1): 11-15

Isolation and Characterization of ACC Deaminase Gene from Two Plant Growth-Promoting Rhizobacteria

2008 ◽  
Vol 57 (4) ◽  
pp. 312-317 ◽  
Author(s):  
Venkadasamy Govindasamy ◽  
Murugesan Senthilkumar ◽  
Kishore Gaikwad ◽  
Kannepalli Annapurna
Keyword(s):  
Plant Growth ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Screening and Whole-Genome Sequencing of Two Streptomyces Species from the Rhizosphere Soil of Peony Reveal Their Characteristics as Plant Growth-Promoting Rhizobacteria

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Chengqiang Wang ◽  
Yun Wang ◽  
Jinjin Ma ◽  
Qihui Hou ◽  
Kai Liu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rhizosphere Soil ◽  
Gc Content ◽  
Plant Growth Promoting Rhizobacteria ◽  
Biological Characteristics ◽  
Protein Coding ◽  
Streptomyces Species ◽  
Protein Coding Genes ◽  
Plant Growth Promoting ◽  
Growth Promoting

Two bacteria, Streptomyces albireticuli MDJK11 and S. alboflavus MDJK44, which are potential plant growth-promoting rhizobacteria against pathogenic fungi were isolated from the rhizosphere soil of peony in Shandong, China. Their biological characteristics and complete genome sequences were reported in this study. The total genome size of MDJK11 was only 8.14 Mb with 6,550 protein-coding genes and a high GC content of 72.8 mol%. The MDJK44 genome comprises a 9.62 Mb chromosome with 72.1 mol% GC content, 7,285 protein-coding genes, and two plasmids. Some gene sequences in these two genomes were analyzed to be heterologously obtained by horizontal transfer. Gene or gene cluster candidates responding to secondary metabolites production, antimicrobial activities, and plant growth-promoting capacities were also analyzed in this paper. The genomic information and biological characteristics will facilitate the understanding and application of S. albireticuli and S. alboflavus species as biocontrol agents in future agriculture.

Sign in / Sign up

Export Citation Format

Share Document