scholarly journals Screening and Whole-Genome Sequencing of Two Streptomyces Species from the Rhizosphere Soil of Peony Reveal Their Characteristics as Plant Growth-Promoting Rhizobacteria

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Chengqiang Wang ◽  
Yun Wang ◽  
Jinjin Ma ◽  
Qihui Hou ◽  
Kai Liu ◽  
...  

Two bacteria, Streptomyces albireticuli MDJK11 and S. alboflavus MDJK44, which are potential plant growth-promoting rhizobacteria against pathogenic fungi were isolated from the rhizosphere soil of peony in Shandong, China. Their biological characteristics and complete genome sequences were reported in this study. The total genome size of MDJK11 was only 8.14 Mb with 6,550 protein-coding genes and a high GC content of 72.8 mol%. The MDJK44 genome comprises a 9.62 Mb chromosome with 72.1 mol% GC content, 7,285 protein-coding genes, and two plasmids. Some gene sequences in these two genomes were analyzed to be heterologously obtained by horizontal transfer. Gene or gene cluster candidates responding to secondary metabolites production, antimicrobial activities, and plant growth-promoting capacities were also analyzed in this paper. The genomic information and biological characteristics will facilitate the understanding and application of S. albireticuli and S. alboflavus species as biocontrol agents in future agriculture.

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Cheryl L. Patten ◽  
Haeyoung Jeong ◽  
Andrew J. C. Blakney ◽  
Natalie Wallace

We report here the draft genome sequence ofPseudomonas syringaeGR12-2, a nitrogen-fixing, plant growth–promoting bacterium, isolated from the rhizosphere of an Arctic grass. The 6.6-Mbp genome contains 5,676 protein-coding genes, including a nitrogen-fixation island similar to that inP. stutzeri.


Author(s):  
Zhenzhen Zhao ◽  
Norbert Bokros ◽  
Seth DeBolt ◽  
Piao Yang ◽  
Xia Ye

We report here the genome sequence of Bacillus sp. RRD69, a plant growth-promoting bacterial endophyte isolated from switchgrass plants grown on a reclaimed coal-mining site in Kentucky. RRD69 is predicted to contain 3,758 protein-coding genes with a genome size of 3.715 Mbp and a 41.41% GC content.


2011 ◽  
Vol 356-360 ◽  
pp. 240-243
Author(s):  
Chang Hong Guo ◽  
Xu Han Shi ◽  
Jia Li Liu

A bacterial strain 3-1 which could use ACC as the sole nitrogen sources was isolated from Chinese chives rhizosphere soil. The strain was identified as Pseudomonas fluorescent, according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. The ACC deaminase activity of Pseudomonas fluorescent 3-1 strain had high level of ACC deaminase activity, 2.63±0.12 µmol α-KA • (mg Pr •h)-1 . The procedure described here was relatively straight forward to isolate and characterize new PGPR strains. This bacterium may be used to facilitate the growth of Porrum.


2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.


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