Evaluation on Biocompatibility of Materials of Goose-Head Nail Steel Plates

2012 ◽  
Vol 442 ◽  
pp. 71-75
Author(s):  
Ming Gao ◽  
Qing Fang Liu ◽  
Yao Zhao
Keyword(s):  
Chromosome Aberration ◽  
Ames Test ◽  
Local Reaction ◽  
Steel Plates ◽  
Reaction Test ◽  
Irritation Test ◽  
Test Materials ◽  
Chromosome Aberration Test

Aim of this study was to detect biocompatibility of the materials of goose-head nail steel plates, so as to evaluate the safety of these kind of materials. We combined sensitization test,intracutaneous irritation test,chromosome aberration test,Ames test and a local reaction test after implantation to evaluate the biocompatibility of the test samples. The results suggest that the test samples have no sensitization, no intracutaneous irritation, no genotoxicity in chromosome aberration test and Ames test. Materials of Goose-head Nail Steel Plates are biologically safe and could be generalized for clinic application.

Genotoxicity Studies on Sel-Plex®, a Standardized, Registered High-Selenium Yeast

2006 ◽  
Vol 25 (6) ◽  
pp. 477-485 ◽  
Author(s):  
James C. Griffiths ◽  
Ray A. Matulka ◽  
Ronan Power
Keyword(s):  
Chromosome Aberration ◽  
Micronucleus Test ◽  
Mutagenic Activity ◽  
Human Lymphocytes ◽  
Selenium Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Reverse Mutation ◽  
High Selenium ◽  
Chromosome Aberration Test

Selenium, recognized as an essential nutrient for human health, is a component of proteins and enzymes required for various biological functions and is currently being used as a feed supplement for livestock in geographical areas that are naturally low in selenium. Selenium is structurally similar to sulfur, replacing the sulfur atom in stoichiometric amounts and thus functions through an association with proteins, termed selenoproteins. In geographic areas low in selenium, there is the potential for animals (including humans) to become selenium deficient and this potential deficiency can be remedied by consumption of exogenous selenium, including selenium-enriched yeast ( Saccharomyces cerevisiae) that contains high levels of organic selenium (e.g., selenized yeast). A unique, standardized, registered high selenium food-grade baker’s yeast ( S. cerevisiae; Sel-Plex®), was tested in the following battery of Genotoxicity assays; (1) a bacterial reverse mutation test (Ames test); (2) an in vitro mammalian chromosome aberration test; and (3) a mouse micronucleus test. Under the conditions of this assay, Sel-Plex® showed no evidence of mutagenic activity in Salmonella typhimurium, in the bacterial reverse mutation test. Sel-Plex® did not induce significant chromosomal aberrations in cultured human lymphocytes in the in vitro mammalian chromosome aberration test. Sel-Plex® did not statistically increase the frequency or proportion of micronucleated immature erythrocytes in the mouse micronucleus test. Thus, from the studies presented here, the authors conclude that Sel-Plex® is nongenotoxic.

scholarly journals MUTAGENICITY STUDY OF GADOBENATE DIMEGLUMINE FORMULATION (E7155) (2) : CHROMOSOME ABERRATION TEST WITH HUMAN LYMPHOCYTES IN CULTURE

1999 ◽  
Vol 24 (SupplementI) ◽  
pp. 95-101 ◽  
Author(s):  
Naoki TORITSUKA ◽  
Hirohiko DAIMON ◽  
Shigeki SAWADA ◽  
Fumio SAGAMI ◽  
Piero TIRONE ◽  
...  
Keyword(s):  
Chromosome Aberration ◽  
Human Lymphocytes ◽  
Gadobenate Dimeglumine ◽  
Chromosome Aberration Test

Genotoxicity evaluation of dental restoration nanocomposite using comet assay and chromosome aberration test

2012 ◽  
Vol 24 (1) ◽  
pp. 015105 ◽  
Author(s):  
Marahaini Musa ◽  
Kannan Thirumulu Ponnuraj ◽  
Dasmawati Mohamad ◽  
Ismail Ab Rahman
Keyword(s):  
Comet Assay ◽  
Chromosome Aberration ◽  
Dental Restoration ◽  
Chromosome Aberration Test

The Effects of Vehicles on the Human Dermal Irritation Potentials of Allyl Esters

2006 ◽  
Vol 25 (3) ◽  
pp. 183-193 ◽  
Author(s):  
Valerie T. Politano ◽  
Daniel A. Isola ◽  
Jon Lalko ◽  
Anne Marie Api
Keyword(s):  
Allyl Alcohol ◽  
Human Subjects ◽  
Skin Irritation ◽  
Diethyl Phthalate ◽  
Intended Application ◽  
Dermal Irritation ◽  
Irritation Test ◽  
Solvent Systems ◽  
Test Materials ◽  
Allyl Esters

Allyl esters, frequently used in the fragrance industry, often contain a certain percentage of free allyl alcohol. Allyl alcohol is known to have a potential for delayed skin irritation. Also present in the finished product are different solvent systems, or vehicles, which are used to deliver the fragrances based upon their intended application. This study was conducted to determine whether different vehicles affect the skin irritation potential of five different allyl esters. The allyl esters tested were allyl amyl glycolate, allyl caproate, allyl (cyclohexyloxy)acetate, allyl cyclohexylpropionate, and allyl phenoxyacetate in the vehicles diethyl phthalate, 3:1 diethyl phthalate:ethanol, and 1:3 diethyl phthalate:ethanol at concentrations of 0.1%, 0.5%, 1.0%, and 2.0% ( w/w). A modified cumulative irritation test was conducted in 129 human subjects. Test materials (0.3 ml) were applied under occlusion to skin sites on the back for 1 day (24 h) using Hill Top chambers. Irritation was assessed at 1, 2, 4, and 5 days following application of test materials. Cumulative irritation scores varied considerably among test materials. There were no delayed irritation observations. The highest irritation scores were observed at the 2.0% concentration for all test materials. The irritation scores for allyl amyl glycolate, allyl (cyclohexyloxy)acetate, and allyl phenoxyacetate were highest in 1:3 diethyl phthalate:ethanol, thus the resulting calculated no-observed-effect levels, 0.12%, 0.03%, and 0%, respectively, were much lower for this vehicle compared to the diethyl phthalate vehicle, 0.33%, 0.26%, 0.25%, respectively. These data showed a trend for lower concentration thresholds to induce irritation when higher levels of ethanol were used in the vehicle.

scholarly journals In Vitro Genotoxicity Assessment from the Glycyrrhiza New Variety Extract

2021 ◽  
Vol 11 (21) ◽  
pp. 10257
Author(s):  
Young-Jae Song ◽  
Dong-Gu Kim ◽  
Jeonghoon Lee ◽  
Wonnam Kim ◽  
Hyo-Jin An ◽  
...  
Keyword(s):  
Ames Test ◽  
Micronucleus Test ◽  
Herbal Medicines ◽  
Mouse Bone Marrow ◽  
New Variety ◽  
Polychromatic Erythrocytes ◽  
Chromosome Aberration Test ◽  
In Vitro Genotoxicity

The various species that comprise the genus Glycyrrhiza (Licorice) have long been used as oriental herbal medicines in Asian countries. Wongam (WG), which is a new variety of Glycyrrhiza, was developed in Korea to overcome the limitations of low productivity, environmental restrictions, and an insufficient presence of glycyrrhizic acid and liquiritigenin. In this study, we evaluated WG extract’s genotoxicity through an in vitro bacterial reverse mutation (AMES) test, an in vitro chromosome aberration test, and an in vivo mouse bone marrow micronucleus test. In the AMES test, WG extract at concentrations of up to 5000 µg/plate showed no genotoxicity regardless of S9 mix. No chromosome aberrations appeared after 6 h in 1400 µg/mL WG extract regardless of S9 mix or in 1100 µg/mL WG extract after 24 h without S9 mix. Nor was there a significant increase in the number of micronucleated polychromatic erythrocytes to total erythrocytes up to 5000 mg/kg/day for 2 days detected in the micronucleus test. These results confirm that WG extract is safe for use as an herbal medicine, as it precipitates no detectable genotoxic effects.

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