Performance of Denitrifying Phosphorous Removal Process Employing Nitrite as Electron Acceptor and the Characterization of Dominant Functional Populations

2012 ◽  
Vol 455-456 ◽  
pp. 1019-1024 ◽  
Author(s):  
Hong Xu Bao ◽  
Xi Ping Ma ◽  
Jian Wang ◽  
Kui Jing ◽  
Man Li Shen ◽  
...  

A sequencing batch reactors (SBR) was adopted to investigate the denitrifying phosphorus removal efficiency employing nitrite as electron acceptor under anaerobic/anoxic condition. The experimental results showed that high nitrogen and phosphorus removal efficiency could be obtained under the following conditions: nitrite concentration of 30~40 mg/L, COD concentration of 400 mg/L, pH 8.0±0.2 in anaerobic stage and pH 7.2±0.2 in anoxic stage, sludge retention time (SRT) of 22 days. When the reactor performed steadily, a dominant functional strain was screened from the activated sludge, which has high nitrite and phosphorus removal efficiency. Batch tests results showed that the removal degree of nitrite and phosphorus could reach 99.18% and 84.94% respectively when their concentrations were 20mg/L and 10mg/L. according to the morphology and physio-biochemical characteristics, and the results of 16S rDNA sequencing, it is determined that the strain belongs to the Genus of Sphingobacterium. The experimental results achieved in this study might offer guidance to the development of shortcut denitrifying phosphorus removal process.

2001 ◽  
Vol 43 (3) ◽  
pp. 139-146 ◽  
Author(s):  
W. J. Ng ◽  
S. L. Ong ◽  
J. Y. Hu

Denitrifying phosphorus removal was verified in a laboratory anaerobic/anoxic Sequencing Batch Reactor (A/A SBR) for a period of 18 months. The results obtained demonstrated the ability of the anaerobic/anoxic strategy to enrich the growth of denitrifying phosphorus bacteria (DPB) capable of taking up phosphate under anoxic conditions by using nitrate as the electron acceptor. Phosphorus removal efficiency ranging from 40-100% could be attained in an A/A SBR system. Simultaneous anoxic phosphate uptake and biological denitrification under anaerobic/anoxic condition occurred in this system. Batch tests showed, however, that simultaneous presence of carbon and nitrate would be detrimental to denitrifying phosphorus removal. Results of bacteria studies suggested that three denitrifying isolates had aerobic phosphorus removing ability.


2007 ◽  
Vol 52 (16) ◽  
pp. 2179-2183 ◽  
Author(s):  
RongXin Huang ◽  
Dong Li ◽  
XiangKun Li ◽  
LinLin Bao ◽  
AnXi Jiang ◽  
...  

2013 ◽  
Vol 726-731 ◽  
pp. 2156-2159 ◽  
Author(s):  
Jing Ni Xiao ◽  
Li Na Zheng ◽  
Lei Zhang ◽  
Han Min Zhang ◽  
Feng Lin Yang

The phosphorus uptake rate characteristics have been investigated in different electron acceptor conditions (NO3-, O2, O2 and NO3- coexisting). The sludge was transferred from CAS, AO MBR, AOA MBR, A2O MBR or the A, B tank of MUCT-MBR systems. The results show that the phosphorus uptake rate (SPUR) have the same rule for the sludge in different electron acceptors, that is NSPUR (NO3- as the electron acceptor) <ASPUR (O2 as the electron acceptor) <TSPUR (both O2 and NO3- as the electron acceptors). There exists the aerobic denitrifying phosphorus removal process in mixed electron acceptor system. And this process shows a positive correlation with the ability of denitrifying phosphorus removal improved. The higher ability of denitrifying phosphorus uptake the sludge have, the faster nitrate consumption rate could be shown through the phosphorus uptake process under the condition of in O2 and NO3- coexisting. That is TSDNR(AO)<TSDNR(AOA)<TSDNR(A2O). The relationship between the nitrate consumption rates is ASDNR (only O2 exist) <TSDNR (TP and O2 coexist) <NSDNR (only TP exist). The phosphorus removal mechanism might include three parts: the aerobic phosphorus uptake, the anoxic denitrifying phosphorus uptake and the aerobic denitrifying phosphorus uptake.


2016 ◽  
Vol 18 (2) ◽  
pp. 251-258 ◽  

<div> <p>Denitrifying polyphosphate accumulating organisms (DNPAOs) are very promising for simultaneous nitrogen and phosphorus removal. While during denitrification, emission of a greenhouse gas, nitrous oxide (N<sub>2</sub>O), may occur. In this study, DNPAOs were enriched in a lab-scale reactor, and N<sub>2</sub>O emission was examined under different electron acceptor conditions. During the anoxic phase, with the uptake of phosphorus, denitrification of nitrate nitrogen (NO<sub>3</sub>-N) was observed without the accumulation of nitrite nitrogen (NO<sub>2</sub>-N). In general, a very low amount of N<sub>2</sub>O was produced with nitrate as the electron acceptor, independent of the applied different nitrate concentrations. However, with nitrite as the electron acceptor, a much higher N<sub>2</sub>O emission occurred. The N<sub>2</sub>O emission factor to the denitrified NO<sub>2</sub>-N was 6.2%, 5.3% and 4.9% at the initial NO<sub>2</sub>-N concentration of 10, 20 and 40 mg l<sup>-1</sup>, respectively. In addition, a much higher N<sub>2</sub>O emission occurred with the co-existence of NO<sub>3</sub>-N and NO<sub>2</sub>-N. The initial organic carbon concentration had no significant effect on N<sub>2</sub>O emission with NO<sub>3</sub>-N as the electron acceptor. When stored organic carbon by DNPAOs was used as the electron donor, N<sub>2</sub>O emission was mainly dependent on the electron acceptor.&nbsp;</p> </div> <p>&nbsp;</p>


2012 ◽  
Vol 518-523 ◽  
pp. 440-443
Author(s):  
Yu Jiao Luo ◽  
Ling Feng Qiu ◽  
Yi Ming Chen ◽  
Jian Zhang

Based on DPBs (Denitrifying Phosphorus-removing Bacteria) obtained from a lab-scale SBR, a quinone profile system had been established to analyze quinones in sludge samples. There existed a positive correlation between the contents of UQ-8 extracted from the sludge samples and the denitrifying and phosphorus removal efficiency of the treating system. With quinone profiles taken as a new important index, it was evidently feasible to determine the removal effect.


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