Denitrifying phosphorus removal by anaerobic/anoxic sequencing batch reactor

2001 ◽  
Vol 43 (3) ◽  
pp. 139-146 ◽  
Author(s):  
W. J. Ng ◽  
S. L. Ong ◽  
J. Y. Hu

Denitrifying phosphorus removal was verified in a laboratory anaerobic/anoxic Sequencing Batch Reactor (A/A SBR) for a period of 18 months. The results obtained demonstrated the ability of the anaerobic/anoxic strategy to enrich the growth of denitrifying phosphorus bacteria (DPB) capable of taking up phosphate under anoxic conditions by using nitrate as the electron acceptor. Phosphorus removal efficiency ranging from 40-100% could be attained in an A/A SBR system. Simultaneous anoxic phosphate uptake and biological denitrification under anaerobic/anoxic condition occurred in this system. Batch tests showed, however, that simultaneous presence of carbon and nitrate would be detrimental to denitrifying phosphorus removal. Results of bacteria studies suggested that three denitrifying isolates had aerobic phosphorus removing ability.

2006 ◽  
Vol 53 (6) ◽  
pp. 21-27 ◽  
Author(s):  
Y. Yoshida ◽  
K. Takahashi ◽  
T. Saito ◽  
K. Tanaka

An anaerobic/aerobic/anoxic/aerobic sequencing batch reactor (SBR) was operated with municipal wastewater to investigate the effect of nitrite on biological phosphorus removal (BPR). When nitrite accumulated, aerobic phosphate uptake activity significantly decreased and, in case of hard exposure to nitrite, BPR severely deteriorated. The interesting observation was that the relative anoxic activity of phosphate accumulating organisms (PAOs) increased after nitrite exposure. Moreover batch tests of aerobic phosphate uptake in the presence/absence of nitrite indicated that PAOs with the higher relative anoxic activity are less sensitive to nitrite exposure. From these results, we concluded that BPR is sensitive to nitrite exposure, but BPR containing PAOs with the higher relative anoxic activity is possibly more stable against nitrite than BPR containing PAOs with the lower relative anoxic activity.


2012 ◽  
Vol 455-456 ◽  
pp. 1019-1024 ◽  
Author(s):  
Hong Xu Bao ◽  
Xi Ping Ma ◽  
Jian Wang ◽  
Kui Jing ◽  
Man Li Shen ◽  
...  

A sequencing batch reactors (SBR) was adopted to investigate the denitrifying phosphorus removal efficiency employing nitrite as electron acceptor under anaerobic/anoxic condition. The experimental results showed that high nitrogen and phosphorus removal efficiency could be obtained under the following conditions: nitrite concentration of 30~40 mg/L, COD concentration of 400 mg/L, pH 8.0±0.2 in anaerobic stage and pH 7.2±0.2 in anoxic stage, sludge retention time (SRT) of 22 days. When the reactor performed steadily, a dominant functional strain was screened from the activated sludge, which has high nitrite and phosphorus removal efficiency. Batch tests results showed that the removal degree of nitrite and phosphorus could reach 99.18% and 84.94% respectively when their concentrations were 20mg/L and 10mg/L. according to the morphology and physio-biochemical characteristics, and the results of 16S rDNA sequencing, it is determined that the strain belongs to the Genus of Sphingobacterium. The experimental results achieved in this study might offer guidance to the development of shortcut denitrifying phosphorus removal process.


2004 ◽  
Author(s):  
◽  
Abel Jwili Manganyi

The objective of this study was to evaluate the characteristics and treatability of process wastewater from an edible oil refining industry, which discharge its effluent into a sewer system. The main objective was to assess a laboratory scale treatment process that would produce effluent having a regulatory acceptable phosphate concentration (below 20 mgIL) prior to discharge into municipal sewer system. A single stage laboratory-scale anaerobic-aerobic sequencing batch reactor (BPR-SBR) with a total volume adjustable up to 10L was designed for biological phosphorus removal. The BPR-SBR was run at 10 days sludge age, 8 hours hydraulic retention time and organic load of ~ 0.38 kg COD/kg MLSS.d for 158 days to evaluate its performance for bio-P removal efficiency. The BPR-SBR system showed a consistent P removal efficiency of up to 78.40 %, 80.15 % COD and 72.43 % FOG reduction. The laboratory scale study has demonstrated that the SBR technology is suitable for treating wastewater from edible oil producing industry.


2010 ◽  
Vol 113-116 ◽  
pp. 1997-2002
Author(s):  
Xin Xin Jiang ◽  
Fang Ma ◽  
Xiao Xin Zhang ◽  
Jun Yin ◽  
Li Wei

Kinetic equations of soluble components are analyzed in an anaerobic/ anoxic sequencing batch reactor (A/A SBR) enriched with denitrifying phosphorus removing bacteria (DPB) sludge. Mathematical model is established for denitrifying phosphorus removal process. The model is based on the simplification of the metabolic model for biological phosphorus removal by DPB. A proper set of kinetic parameters was calculated from the data obtained in the batch tests. The model was subsequently applied for the simulation of cycle behavior of soluble components in the A/A SBR. The results of the simulation indicated that the model can predict the concentration of each component for denitrifying phosphorus removal successfully.


2001 ◽  
Vol 43 (3) ◽  
pp. 191-194 ◽  
Author(s):  
M. Merzouki ◽  
N. Bernet ◽  
J.-P. Deigenès ◽  
R. Moletta ◽  
M. Benlemlih

Optimizing anoxic biological phosphorus removal in the anaerobic-anoxic sequencing batch reactor (A2 SBR) was observed to depend on two parameters: the amount of added nitrate and the sludge retention time (SRT). The concentration of 120 mg N-NO3 · l-1 in the anoxic medium and the SRT of 15 days were determined as optimal for a complete phosphorus removal in the A2 SBR. The reactor was supplied with synthetic wastewater containing 800 mg COD.l-1 acetic acid, 240 mg N-NH4·l-1 and 30 mg P-PO4·l-1. This study was completed by microscopic observations which revealed three morphological types of phosphate-accumulating bacteria (PAB).


2006 ◽  
Vol 53 (9) ◽  
pp. 185-191 ◽  
Author(s):  
Xiaoling Zhang ◽  
Zhiying Wang ◽  
Qing Zhao

Biological phosphorus removal was studied in a sequencing batch reactor (SBR). The results showed that nitrite could be used as electron acceptor in denitrifying phosphorus removal. Feed mode of nitrite had significant influence on denitrifying phosphorus removal. Anoxic phosphorus assimilation rate could reach 10.44 mgP/gSS.h and the percentage of anoxic phosphorus assimilation amount was more than 97% with continuous feed mode. Granular sludge with denitrifying phosphorus removal activity was found in the SBR. The effects of different operational conditions, such as COD loading, settling time, HRT etc., on the formation of granules were also studied.


1994 ◽  
Vol 30 (6) ◽  
pp. 263-269 ◽  
Author(s):  
T. Kuba ◽  
A. Wachtmeister ◽  
M. C. M. van Loosdrecht ◽  
J. J. Heijnen

The effect of nitrate on phosphorus release by biological phosphorus removing organisms has been studied. Denitrifying (DPB) or aerobic phosphorus removing bacteria were enriched in an anaerobic-anoxic or anaerobic-aerobic sequencing batch reactor (SBR). The enrichment sludges were used in batch tests, in which the effect of simultaneous presence of substrate (HAc) and nitrate was studied on the phosphorus release. It could be concluded that a reduction of the phosphorus release by nitrate in biological phosphorus removal systems is partly due to the presence of DPB, which utilize HAc for denitrification, not for phosphorus release. PHB (poly-β-hydroxybutyrate) was always produced and phosphorus was released by DPB sludge when nitrate and HAc were simultaneously present. The reducing power (NADH2) and the energy (ATP) for this process seemed to be obtained from HAc oxidation by nitrate as well as from polyphosphate degradation. After removal of the HAc, PHB degradation and phosphorus uptake occurred.


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