Isolation and Identification of Photosynthetic Bacteria and their Hydrogen Production Characterization

2012 ◽  
Vol 512-515 ◽  
pp. 527-533
Author(s):  
Yan Yan Zhu ◽  
Quan Guo Zhang ◽  
Bin Xu Han ◽  
Yan Yan Jing
Keyword(s):  
Hydrogen Production ◽  
Photosynthetic Bacteria ◽  
Culture Medium ◽  
Gene Sequence ◽  
Rhodospirillum Rubrum ◽  
Rhodobacter Capsulatus ◽  
Solid Medium ◽  
Production Capacity ◽  
Sequence Information ◽  
Isolation And Identification

Using special culture medium, dominant photosynthetic bacterias were enriched from sewage silt and paddy fields with plentiful light and organic compounds. Five strains of photosynthetic bacteria capable of producing hydrogen were purified and isolated by gradual dilution and double—deck solid medium. The analysis of the gene sequence information of 1 6SrDNA was carried out, and the five strains were identified as Rhodospirillum rubrum,R.capsulata, R.pulastris, Rhodobacter capsulatus, Rhodobacter capsulatus . Moreover, it was found that with Optimized medium formula, hydrogen production can be achieved 204h, the maximum hydrogen production capacity of 3.41L, the maximum hydrogen production rate was 44.17ml / (L • h),the highest hydrogen content of 46.73%.

scholarly journals Structural and Functional Analyses of Photosynthetic Regulatory Genes regA and regBfrom Rhodovulum sulfidophilum, Roseobacter denitrificans, and Rhodobacter capsulatus

1999 ◽  
Vol 181 (14) ◽  
pp. 4205-4215 ◽  
Author(s):  
Shinji Masuda ◽  
Yumi Matsumoto ◽  
Kenji V. P. Nagashima ◽  
Keizo Shimada ◽  
Kazuhito Inoue ◽  
...  
Keyword(s):  
Photosynthetic Bacteria ◽  
Rhodobacter Capsulatus ◽  
Photosynthetic Apparatus ◽  
Anaerobic Growth ◽  
Binding Motif ◽  
Sequence Information ◽  
Sensor Kinase ◽  
Rhodovulum Sulfidophilum ◽  
High Level ◽  
Aerobic And Anaerobic

ABSTRACT Genes coding for putative RegA, RegB, and SenC homologues were identified and characterized in the purple nonsulfur photosynthetic bacteria Rhodovulum sulfidophilum and Roseobacter denitrificans, species that demonstrate weak or no oxygen repression of photosystem synthesis. This additional sequence information was then used to perform a comparative analysis with previously sequenced RegA, RegB, and SenC homologues obtained fromRhodobacter capsulatus and Rhodobacter sphaeroides. These are photosynthetic bacteria that exhibit a high level of oxygen repression of photosystem synthesis controlled by the RegA-RegB two-component regulatory system. The response regulator, RegA, exhibits a remarkable 78.7 to 84.2% overall sequence identity, with total conservation within a putative helix-turn-helix DNA-binding motif. The RegB sensor kinase homologues also exhibit a high level of sequence conservation (55.9 to 61.5%) although these additional species give significantly different responses to oxygen. ARhodovulum sulfidophilum mutant lacking regA orregB was constructed. These mutants produced smaller amounts of photopigments under aerobic and anaerobic conditions, indicating that the RegA-RegB regulon controls photosynthetic gene expression in this bacterium as it does as in Rhodobacterspecies. Rhodobacter capsulatus regA- orregB-deficient mutants recovered the synthesis of a photosynthetic apparatus that still retained regulation by oxygen tension when complemented with reg genes fromRhodovulum sulfidophilum and Roseobacter denitrificans. These results suggest that differential expression of photosynthetic genes in response to aerobic and anaerobic growth conditions is not the result of altered redox sensing by the sensor kinase protein, RegB.

Biological Hydrogen Production In China: Past, Present and Future

Solar Energy ◽  
2005 ◽  
Author(s):  
Nanqi Ren ◽  
Yongfeng Li ◽  
Maryam Zadsar ◽  
Lijie Hu ◽  
Jianzheng Li
Keyword(s):  
Hydrogen Production ◽  
Culture Media ◽  
Engineering Application ◽  
Production Capacity ◽  
Clean Energy ◽  
Biohydrogen Production ◽  
Isolation And Identification ◽  
Two Phase ◽  
Anaerobic Process ◽  
High Efficient

As a new clean energy source and important material, the use and demand of hydrogen are increasing-rapidly. So that bio-hydrogen producing technology moves toward cutting down the operation costs in recent years. Biohydrogen production capacity improvement and cost reduction are two key points for industrialization of the process. Biohydrogen production has been studied in China for over 20 years in both photosynthetic hydrogen production and fermentative processes fields. The anaerobic process of fermentative hydrogen production has been developing in China since 1990s. The isolation and identification of high efficient bio-hydrogen production anaerobic bacteria is an important foundation of fermentative bio-hydrogen production process by anaerobic digestion of organic wastewater. The paper focuses on: (1) Fermentative biohydrogen production system, (2) Laboratory experiments and pilot scale tests for continued hydrogen production, (3) Fermentation types and their engineering control, (4) isolation, culture media and characterization of anaerobes, (5) Applications of pure bacteria, (6) Fundamental researches including ecology, genetics and improvements, (7) Development of two-phase anaerobic process of H2-producing and methanogenic phases as, and (8) the integrated processes with bioengineering and wastewater treatments. Recently, the first pilot factory has been costructedin Harbin, China by hydrogen production rate of more than 1200m3/d which located in northeast of China. In photosynthetic hydrogen production filed, study is focused on the fundamentals, engineering application and microbiology. Detailed discussion comes later.

Isolation of Hydrogen-Producing Bacteria Suitable for Immobilization from Anaerobic Sludge

2013 ◽  
Vol 772 ◽  
pp. 849-854 ◽  
Author(s):  
Toshiyuki Nomura ◽  
Hideaki Asano ◽  
Toshiyuki Takayama ◽  
Azusa Naimen ◽  
Hayato Tokumoto ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Culture Medium ◽  
Batch Reactor ◽  
Production Capacity ◽  
Soluble Starch ◽  
Anaerobic Sludge ◽  
Hydrogen Yield ◽  
Polymer Resin ◽  
Rdna Sequencing ◽  
Energy Of Interaction

Highly efficient hydrogen-producing anaerobic microflora was enriched from anaerobic sludge obtained from a methane fermentation plant, with soluble starch as substrate. The effect of culture medium pH on hydrogen production capacity and microbial community was investigated using a batch reactor equipped with a pH controller. The optimal pH was 5.0, the hydrogen yield was 2.59 mol/mol-glucose, and the maximum hydrogen production rate was 123 mL/L-culture/h. Partial 16S rDNA sequencing placed the isolated strain within the Clostridiaceae family. When the isolated strain adhered to polymer resin, the change in free energy of interaction was negative, indicating that the bacteria-resin interaction is thermodynamically favorable, and that the strain will readily immobilize on the resin.

State estimation of a batch hydrogen production process using the photosynthetic bacteria Rhodobacter capsulatus

2010 ◽  
Vol 35 (19) ◽  
pp. 10719-10724 ◽  
Author(s):  
Jamila Obeid ◽  
Jean-Marie Flaus ◽  
Olivier Adrot ◽  
Jean-Pierre Magnin ◽  
John C. Willison
Keyword(s):  
Hydrogen Production ◽  
Production Process ◽  
State Estimation ◽  
Photosynthetic Bacteria ◽  
Rhodobacter Capsulatus

Regulation of nitrogenase in the photosynthetic bacteriumRhodobacter sphaeroidescontainingdraTGandnifHDKgenes fromRhodobacter capsulatus

2001 ◽  
Vol 47 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Alexander F Yakunin ◽  
Alexander S Fedorov ◽  
Tatyana V Laurinavichene ◽  
Vadim M Glaser ◽  
Nikolay S Egorov ◽  
...  
Keyword(s):  
Photosynthetic Bacteria ◽  
Rhodospirillum Rubrum ◽  
Rhodobacter Capsulatus ◽  
Nitrogenase Activity ◽  
Photosynthetic Bacterium ◽  
Adp Ribosylation ◽  
Fe Protein ◽  
Dinitrogenase Reductase ◽  
Different Strains

The photosynthetic bacteria Rhodobacter capsulatus and Rhodospirillum rubrum regulate their nitrogenase activity by the reversible ADP-ribosylation of nitrogenase Fe-protein in response to ammonium addition or darkness. This regulation is mediated by two enzymes, dinitrogenase reductase ADP-ribosyl transferase (DRAT) and dinitrogenase reductase activating glycohydrolase (DRAG). Recently, we demonstrated that another photosynthetic bacterium, Rhodobacter sphaeroides, appears to have no draTG genes, and no evidence of Fe-protein ADP-ribosylation was found in this bacterium under a variety of growth and incubation conditions. Here we show that four different strains of Rba. sphaeroides are incapable of modifying Fe-protein, whereas four out of five Rba. capsulatus strains possess this ability. Introduction of Rba. capsulatus draTG and nifHDK (structural genes for nitrogenase proteins) into Rba. sphaeroides had no effect on in vivo nitrogenase activity and on nitrogenase switch-off by ammonium. However, transfer of draTG from Rba. capsulatus was sufficient to confer on Rba. sphaeroides the ability to reversibly modify the nitrogenase Fe-protein in response to either ammonium addition or darkness. These data suggest that Rba. sphaeroides, which lacks DRAT and DRAG, possesses all the elements necessary for the transduction of signals generated by ammonium or darkness to these proteins.Key words: nitrogenase regulation, nitrogenase modification, photosynthetic bacteria.

Hydrogen production by non-photosynthetic bacteria

1985 ◽  
Vol 10 (4) ◽  
pp. 227-231 ◽  
Author(s):  
S HUANG ◽  
C SECOR ◽  
R ASCIONE ◽  
R ZWEIG
Keyword(s):  
Hydrogen Production ◽  
Photosynthetic Bacteria

Construction of LZU1@WO3 heterojunction photocatalysts: enhanced photocatalytic performance and mechanism insight

2021 ◽  
Vol 45 (36) ◽  
pp. 17025-17036
Author(s):  
Simin Shang ◽  
Huaizhi Yang ◽  
Dajun Shi ◽  
Bowen Dong ◽  
Heling Zhang ◽  
...  
Keyword(s):  
Photocatalytic Activity ◽  
Hydrogen Production ◽  
Hybrid Materials ◽  
Photocatalytic Performance ◽  
Production Capacity ◽  
Simulated Sunlight ◽  
Composite Photocatalysts ◽  
Performance And Mechanism

Our well-designed nano-WO3@LZU1 composite photocatalysts were fully characterized. Under simulated sunlight, the hybrid materials showed much higher photocatalytic activity for BBR degradation and MB degradation than WO3 or LZU1, and improved hydrogen production capacity.

Sign in / Sign up

Export Citation Format

Share Document