Determination of β -Sitosterol with Chemical Course and Material Applications in Jatropha Seed Oil by High Performance Liquid Chromatography

2012 ◽  
Vol 577 ◽  
pp. 69-72 ◽  
Author(s):  
Shu Yu Liu ◽  
Anaerguli Maihemuti
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Seed Oil ◽  
Reversed Phase ◽  
Analytical Recovery ◽  
Relative Standard ◽  
The Mean ◽  
Jatropha Seed

A simple and rapid high performance liquid chromatography (HPLC) assay was developed to identify and measure theβ-sitosterol with chemical course and material applications in jatropha seed oil. The stigmasterol was isolated with a good selectivity by HPLC employing reversed phase C18 columns. The components were separated by mobile phase of methanol-water (99/1, v/v) and detected at 205nm. The quantitation of the stigmasterol was reproducible and the method relative standard deviation is 1.1%. The mean analytical recovery was 96.2%.

Determination of Stigmasterol in Jatropha Seed Oil by High Performance Liquid Chromatography

2011 ◽  
Vol 233-235 ◽  
pp. 1206-1209
Author(s):  
Shu Yu Liu ◽  
Li Jie Sun ◽  
Xin Guo
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Seed Oil ◽  
Reversed Phase ◽  
Analytical Recovery ◽  
Relative Standard ◽  
The Mean ◽  
Jatropha Seed

A simple and rapid high performance liquid chromatography (HPLC) assay was developed to identify and measure the stigmasterol in jatropha seed oil. The stigmasterol was isolated with a good selectivity by HPLC employing reversed phase C18 columns. The components were separated by mobile phase of methanol-water (99/1, v/v) and detected at 202nm. The quantitation of the stigmasterol was reproducible and the method relative standard deviation is 1.6%. The mean analytical recovery was 97.0%．

scholarly journals Development and validation of reversed phase high performance liquid chromatography (RP-HPLC) for quantification of captopril in rabbit plasma

2020 ◽  
Author(s):  
Muhammad Fawad Rasool ◽  
Umbreen Fatima Qureshi ◽  
Nazar Muhammad Ranjha ◽  
Imran Imran ◽  
Mouqadus Un Nisa ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Reversed Phase ◽  
Rabbit Plasma ◽  
Rp Hplc ◽  
Relative Standard

AbstractTh accurate rapid, simple and selective reversed phase high performance liquid chromatography (RP-HPLC) has been established and validated for the determination of captopril (CAP). Chromatographic separation was accomplished using prepacked ODSI C18 column (250 mm × 4.6 mm with 5 μm particle size) in isocratic mode, with mobile phase consisting of water: acetonitrile (60:40 v/v), pH adjusted to 2.5 by using 85% orthophosphoric acid at a flow rate of 1 mL/min and UV detection was performed at 203 nm. RP-HPLC method used for the analysis of CAP in mobile phase and rabbit plasma was established and validated as per ICH-guidelines. It was carried out on a well-defined chromatographic peak of CAP was established with a retention time of 4.9 min and tailing factor of 1.871. The liquid–liquid extraction method was used for extraction of CAP from the plasma. Excellent linearity (R2 = 0.999) was shown over range 3.125–100 µg/mL with mean percentage recoveries ranges from 97 to 100.6%. Parameters of precision and accuracy of the developed method meet the established criteria. Intra and inter-day precision (% relative standard deviation) study was also performed which was less than 2% which indicate good reproducibility of the method. The limit of detection (LOD) and quantification for the CAP in plasma were 3.10 and 9.13 ng/mL respectively. The method was suitably validated and successfully applied to the determination of CAP in rabbit plasma samples.

Determination of beta-carotene and its cis isomers in serum

1990 ◽  
Vol 36 (11) ◽  
pp. 1986-1989 ◽  
Author(s):  
W G Rushin ◽  
G L Catignani ◽  
S J Schwartz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Serum ◽  
High Performance ◽  
Beta Carotene ◽  
Reversed Phase ◽  
Serum Samples ◽  
Analytical Recovery ◽  
Cis Isomer

Abstract All-trans-beta-carotene was resolved from its cis isomers in human serum by reversed-phase "high-performance" liquid chromatography. Absorption spectra of the cis peak suggested that 13-cis-beta-carotene was the predominant cis isomer. Analyses and recovery studies of fresh and stored sera eliminated the possibility that isomerization had occurred in samples during handling or storage. The average analytical recovery was 101.9% for standards of the all-trans-, 9-cis-, and 13-cis-beta-carotene compounds in pooled serum samples. We also demonstrated that cis isomers had not formed after the blood was drawn and that cis isomers of beta-carotene are present at significant concentrations in the human circulation.

High-Performance Liquid Chromatography Multiresidue Method for the Determination of N-Methyl Carbamates in Fruit and Vegetable Juices

2004 ◽  
Vol 67 (11) ◽  
pp. 2565-2569 ◽  
Author(s):  
CONSUELO SÁNCHEZ-BRUNETE ◽  
BEATRIZ ALBERO ◽  
JOSÉ LUIS TADEO
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Fruit And Vegetable ◽  
Multiresidue Method ◽  
Relative Standard ◽  
Fruit And Vegetable Juices ◽  
Postcolumn Derivatization

A rapid multiresidue method has been developed for the analysis of N-methylcarbamate insecticides (oxamyl, methomyl, propoxur, carbofuran, carbaryl, and methiocarb) in fruit and vegetable juices. The method is based on the adsorption of the N-methyl carbamates in Florisil and the subsequent extraction of pesticides using a low volume of acetone. Residue levels in juice were determined by reversed-phase high-performance liquid chromatography with fluorescence detection after postcolumn derivatization. The separation of carbamates is performed on a C8 column with water-methanol as mobile phase. Recovery studies were performed at 500-, 100-, and 10-ng/ml fortification levels, and average recoveries obtained for carbamates ranged from 79 to 109%, with relative standard deviations between 1.4 and 9.9%. The method was found to be linear over the range assayed from 10 to 1,000 ng/ml, and the detection limits for carbamates varied from 0.8 to 1.9 ng/ml.

scholarly journals Reversed-Phase High-Performance Liquid Chromatography Determination of Selected Phenolic Acids in Propolis Concentrates in Terms of Standardization For Drug Manufacturing Purposes

2006 ◽  
Vol 89 (2) ◽  
pp. 352-358 ◽  
Author(s):  
Jan Krzek ◽  
Jolanta Kaleta ◽  
Urszula Hubicka ◽  
Aneta Niedzwiedz
Keyword(s):  
Antibacterial Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Solid Phase ◽  
Reversed Phase ◽  
Good Precision ◽  
Relative Standard ◽  
Ferulic Acids

Abstract A reversed-phase high-performance liquid chromatography method with gradient elution was developed for the determination of the caffeic, p-coumaric, and ferulic acids in propolis concentrates. Solid-phase extraction on an RP18 column was applied for preliminary purification, and chromatographic separation was performed on 100 RP18e Lichrospher column of particle size 5 m. The mobile phase was obtained by mixing in appropriate ratios 0.03 mM NaH2PO4, acidified with H3PO4 up to pH 3.0, with acetonitrile to obtain a gradient in the elution process. Spectrophotometric detection was conducted at 320 nm. Under the established conditions, the method featured high sensitivity, good precision, and comparability of results, as proven by method validation and statistical analysis of the obtained results. The limits of detection were 0.315, 0.325, and 0.695 g/mL for caffeic, p-coumaric, and ferulic acids, respectively. The corresponding recovery values were 98.14, 101.05, and 99.42 and the linearity ranges from 1.31 to 99.18 g/mL, 1.52 to 119.16 g/mL, and 2.42 to 184.14 g/mL. The precision of the method was expresed by relative standard deviation values that did not exceed 3. It was also shown that the propolis concentrates under examination had similar antibacterial activity against Staphylococcus aureus ranging from 119.8 to 124.3 g/mL, contrary to model mixtures that showed no antibacterial activity.

scholarly journals Determination of dehydroepiandrosterone in dietary supplements by reversed-phase HPLC

2022 ◽  
Vol 20 (2) ◽  
pp. 383-387
Author(s):  
Yahia Z. Tabaza ◽  
Kamal M. Mansi ◽  
Hanan A. Azzam ◽  
Farah F. Al-Mamoori ◽  
Ali M. Al-Samydai ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Dietary Supplements ◽  
High Performance ◽  
Cost Effective ◽  
Reversed Phase ◽  
Hplc Method ◽  
Time Saving ◽  
Relative Standard

Purpose: To develop a reversed phase high performance liquid chromatography (HPLC) method for the determination of dehydroepiandrosterone (DHEA) in dietary supplements. Methods: A reversed-phase high performance liquid chromatography (HPLC) method was developed for the determination of DHEA in dietary supplements. An isocratic system consisting of methanol and water (70:30 v/v) was run at a flow rate of 1 mL/min on a C18 HPLC column to achieve the separation. The method was validated with regard to linearity, intra-day and inter-day precision, and limits of both detection and quantification. Results: The method achieved a retention time of 10.8 min, a resolution of 4.12, a detection limit (LOD) of 50 ng/μL, a quantification limit (LOQ) of 166.7 ng/μL and a label claim of 108.6 % with a relative standard deviation (RSD) of 0.38 % over a range of 0.0625 – 0.50 mg/mL with a correlation coefficient of 0.9997. Conclusion: The method is simple, cost effective, time-saving and reliable for determining DHEA when compared to other reported methods in literature. Thus, it will be of benefit to manufacturers of this dietary supplement to adopt the method for quantitative laboratory analysis.

scholarly journals Stability-Indicating High-Performance Liquid Chromatography Assay for the Determination of Sulthiame in Pharmaceutical Dosage Forms

2016 ◽  
Vol 11 ◽  
pp. ACI.S38656 ◽  
Author(s):  
Ammar Haidar ◽  
Sofiane Kabiche ◽  
Elyes Majoul ◽  
Issa-Bella Balde ◽  
Jean-Eudes Fontan ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Coefficient Of Determination ◽  
Pharmaceutical Dosage Forms ◽  
Reliable Determination ◽  
Stability Indicating ◽  
Relative Standard

A stability-indicating assay by reversed-phase high performance liquid chromatography method was developed and validated for the determination of sulthiame (STM). The chromatographic separation was achieved on a reversed-phase NovaPack C18 column and an isocratic mobile phase consisting of deionized watenmethanol (70:30, v/v). The flow rate was 1.0 mL/min (ultraviolet detection at 210 nm). The STM was separated within 2.83 min. The linearity of the method was demonstrated in the range of 20.0-200.0 μg/mL and a coefficient of determination of r 2 = 0.9999. The limits of detection and quantification were 4.2 and 9.5 μg/mL, respectively. The intraday and interday precisions were less than 1%. Accuracy of the method ranged from 98.3% to 101.7%, with a relative standard deviation of <1%. STM was degraded by accelerated breakdown in alkaline, acidic, or oxidative stress conditions. This method allows accurate and reliable determination of STM for drug stability assay in pharmaceutical studies.

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