Cytochrome P450 Content in Gut of Earthworm (Eisenia fetida) for Monitoring Pahs Contaminated Soil

2013 ◽  
Vol 726-731 ◽  
pp. 1468-1474 ◽  
Author(s):  
Wei Zhang ◽  
Yu Fang Song ◽  
Ming Da Liu ◽  
Yu Shan Qi
Keyword(s):  
Cytochrome P450 ◽  
Aromatic Hydrocarbons ◽  
Eisenia Fetida ◽  
Specific Activity ◽  
Dry Weight ◽  
Sublethal Dose ◽  
High Concentration ◽  
Potential Biomarker ◽  
Polycyclic Aromatic ◽  
Earthworm Gut

Polycyclic aromatic hydrocarbons (PAH) are known to be pollutants in soil, however there are no effective methods available for determining their ecotoxic effects. We investigated Cytochrome P450 (P450) in earthworms (Eisenia fetida) as a potential biomarker of PAH pollutants in soil. Three types of PAHs were tested which included phenanthrene (Phe), fluoranthene (Fla) and pyrene (Py). Earthworm gut was selected to measure the total P450 content in cellular microsomes. Dose-response relationships between total P450 content in earthworm gut and PAH concentration (60, 120, 240, 480 and 960μg kg-1soil (dry weight)) were determined for different exposure durations (1, 3, 7 and 14 days). Results showed that the content of P450 in earthworm changed with exposure to each of PAHs. Biphasic effects were identified in response to each of PAHs tested, in which total P450 content increased due to short, low concentration doses, and decreased due to longer, high concentration doses. Alterations to total P450 content in the presence of PAHs, show that it is a promising biomarker for identifying ecotoxic pollutants at sublethal dose ranges in soil. Further research to identify the specific activity of P450 isoenzymes could further develop and also complement this tool.

Potential Contribution of Nutrients and Polycyclic Aromatic Hydrocarbons from the Creeks of Cootes Paradise Marsh

1996 ◽  
Vol 31 (3) ◽  
pp. 485-504 ◽  
Author(s):  
Patricia Chow-Fraser ◽  
Barb Crosbie ◽  
Douglas Bryant ◽  
Brian McCarry
Keyword(s):  
Aromatic Hydrocarbons ◽  
Laboratory Experiments ◽  
Dry Weight ◽  
Common Source ◽  
Potential Contribution ◽  
Nitrogen And Phosphorus ◽  
Engine Combustion ◽  
Polycyclic Aromatic ◽  
High Concentrations ◽  
Derivatives Of

Abstract During the summer of 1994, we compared the physical and nutrient characteristics of the three main tributaries of Cootes Paradise: Spencer, Chedoke and Borer’s creeks. On all sampling occasions, concentrations of CHL α and nutrients were always lowest in Borer’s Creek and highest in Chedoke Creek. There were generally 10-fold higher CHL α concentrations and 2 to 10 times higher levels of nitrogen and phosphorus in Chedoke Creek compared with Spencer Creek. Despite this, the light environment did not differ significantly between Spencer and Chedoke creeks because the low algal biomass in Spencer Creek was balanced by a relatively high loading of inorganic sediments from the watershed. Laboratory experiments indicated that sediments from Chedoke Creek released up to 10 µg/g of soluble phosphorus per gram (dry weight) of sediment, compared with only 2 µg/g from Spencer Creek. By contrast, sediment samples from Spencer Creek contained levels of polycyclic aromatic hydrocarbon that were as high as or higher than those from Chedoke Creek, and much higher than those found in Borer’s Creek. The distribution of normalized PAH concentrations suggests a common source of PAHs in all three tributaries, most likely automobile exhaust, since there were high concentrations of fluoranthene and pyrene, both of which are derivatives of engine combustion.

Polycyclic Aromatic Hydrocarbons (PAHs) in Sediments of the Brisbane River (Australia) – Preliminary Results

1989 ◽  
Vol 21 (2) ◽  
pp. 161-165 ◽  
Author(s):  
S. I. Kayal ◽  
D. W. Connell
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
River Estuary ◽  
Dry Weight ◽  
Weight Basis ◽  
Sediment Samples ◽  
Preliminary Results ◽  
Treated Sewage ◽  
Polycyclic Aromatic ◽  
Petroleum Refineries

Results of the analysis of twenty-three composite sediment samples revealed that PAHs are widely distributed in the Brisbane River estuary. Mean concentrations for individual compounds, on a dry weight basis, ranged from 0.03 µg/g for dibenz [ah] anthracene to 2.34 µg/g for fluoranthene. Observed PAH assemblages were rich in compounds having pyrolytic origins. However, the presence of petroleum derived compounds was indicative of the importance of petroleum as a PAH source in the estuary. Petroleum refineries, a coal loading terminal and a major treated sewage outfall located at the mouth were not indicated as major contributing sources of PAH pollution in the estuary.

Bioaccumulation of polycyclic aromatic hydrocarbons by earthworms Eisenia fetida

2011 ◽  
Vol 205 ◽  
pp. S120
Author(s):  
M. Beklova ◽  
M. Vavrova ◽  
M. Stoupalova ◽  
I. Soukupova ◽  
E. Stilarkova ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Eisenia Fetida ◽  
Polycyclic Aromatic

Non-specific cellular function of Eisenia fetida regulated by polycyclic aromatic hydrocarbons

Pedobiologia ◽  
2003 ◽  
Vol 47 (5-6) ◽  
pp. 717-723
Author(s):  
Kazuo Komiyama ◽  
Masashiro Okaue ◽  
Yukako Miki ◽  
Mitsuo Ohkubo ◽  
Itaru Moro ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Eisenia Fetida ◽  
Cellular Function ◽  
Polycyclic Aromatic

Determination of biomarkers for polycyclic aromatic hydrocarbons (PAHs) toxicity to earthworm (Eisenia fetida)

2015 ◽  
Vol 37 (6) ◽  
pp. 943-951 ◽  
Author(s):  
Tae-Hoon Nam ◽  
Hwang-Ju Jeon ◽  
Hyung-ho Mo ◽  
Kijong Cho ◽  
Yong-Sik Ok ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Eisenia Fetida ◽  
Polycyclic Aromatic

Biodegradation of Polycyclic Aromatic Hydrocarbons Using FungiNew Prospects toward Cytochrome P450 Engineering

2018 ◽  
pp. 417-445
Author(s):  
Ulises Conejo-Saucedo ◽  
Darío Rafael Olicón-Hernández ◽  
Haley Paula Stein ◽  
Jesús González-López ◽  
Elisabet Aranda
Keyword(s):  
Cytochrome P450 ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic

scholarly journals Tracing historical changes, degradation, and original sources of airborne polycyclic aromatic hydrocarbons (PAHs) in Jilin Province, China, by Abies holophylla and Pinus tabuliformis needle leaves

2021 ◽  
Author(s):  
Zhao Wang ◽  
Xiangzi Jin ◽  
Han Yeong Kaw ◽  
Zakia Fatima ◽  
Maurizio Quinto ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Dry Weight ◽  
Air Pollutant ◽  
Jilin Province ◽  
General Tendency ◽  
Coal Consumption ◽  
Pinus Tabuliformis ◽  
Degradation Rates ◽  
Polycyclic Aromatic

Abstract Due to their wide distribution and availability, plant leaves can be considered interesting candidates as biomonitoring substrates for the evaluation of atmospheric pollution. In addition, some species can also retain historical information, for example, related to environmental pollution, due to their leaf class age. In this study, the content of polycyclic aromatic hydrocarbons (PAHs) in Abies holophylla and Pinus tabuliformis needle samples in function of their class age has been investigated to obtain information regarding the degradation constant for each PAH under investigation (α values ranging from 0.173 to 1.870) and to evaluate the possibility to correlate the presence of PAHs in needles with some important pollution environmental factors. Considering air pollutant variables registered in Jilin Province, significant correlations (at 95% confidence level) have been found between coal consumption per year and anthracene contents in needles, while fluorene, phenanthrene, and anthracene resulted correlated with coal consumption. Furthermore, it has been demonstrated that the total PAH concentration in needles, for both species, increased with their age (from 804 to 3604 ng g− 1 dry weight), showing a general tendency to accumulate these substances through years. PAH degradation rates increased instead with molecular complexity. This study could be considered a first trial to obtain historical environmental information by pine needles biomonitoring.

scholarly journals Occurrence and Distribution of Polycyclic Aromatic Hydrocarbons in Water and Sediment Collected along the Harbour Line, Mumbai, India

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
V. Dhananjayan ◽  
S. Muralidharan ◽  
Vinny R. Peter
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Sediment Quality ◽  
Quality Criteria ◽  
Marine Organisms ◽  
High Concentration ◽  
Sediment Samples ◽  
Water And Sediment ◽  
Polycyclic Aromatic ◽  
Water And Sediment Samples

This study investigated the occurrence of polycyclic aromatic hydrocarbons (PAHs) in water and sediment samples collected along the harbour line, Mumbai, India. The ∑PAHs quantified in water and sediment samples were ranged from 8.66 ng/L to 46.74 ng/L and from 2608 ng/g to 134134 ng/g dry wt., respectively. Significantly high concentration of ∑PAHs was found in water samples of Sewri and sediment samples of Mahul (P<0.05). PAH concentrations detected in the present study were several folds higher than the existing sediment quality criteria suggested by various statutory agencies. The PAH composition patterns in water and sediments suggest the dominance of high molecular weight compounds and indicate important pyrolytic and petrogenic sources. The occurrence of PAHs in the marine environment has attracted the attention of the scientific community as these compounds are frequently detected in seawater and sediments at increasing levels and can have adverse health effects on marine organisms and humans. PAH concentrations detected at Sewri-Mahul site were sufficiently high to pose a risk to marine organisms if they are exposed continuously to this concentration. Hence, continuous monitoring of the ecosystem is highly warranted.

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