Measurement of Absorption Spectrum of Media by Grating Spectrometer

2010 ◽  
Vol 663-665 ◽  
pp. 1137-1141
Author(s):  
Han Chen Liu ◽  
Xiang An Yan ◽  
Chang Jun Zhu ◽  
Cai Juan Xia ◽  
Hua Wa Yu ◽  
...  

In order to measure quantum yield (quantum efficiency) of light in the research of fluorescence excitation, rapid prototyping of micro-structure and photochemical, the absorption spectrum must be precisely measured. Therefore, the reflection on optical media interface, absorption and transmission in the medium must also be considered. In this article, we exhibit a set of reasonable experimental method which is designed to measure absorption spectrum by grating spectrometer. The results show that the obtained absorption lines are properly reliable if we select an appropriate way to settle the sample. Furthermore, it can be well generalized and applied to the selection of fluorescence excitation spectrum, photochemical reaction spectrum, photoelectric device spectrum.

Author(s):  
M. N. Merzlyak ◽  
O. B. Chivkunova ◽  
S. I. Pogosyan ◽  
L. I. Kuznetsova ◽  
N. P. Ponomareva ◽  
...  

SynopsisThe oxidation products of unsaturated hydrocarbon α-farnesene that accumulate in cuticular lipids of stored apple fruits and are involved in the development of superficial scald showed UV fluorescence. The fluorescence emission band was localised at about 330 nm; the fluorescence excitation spectrum resembles the absorption spectrum of the products, but was more resolved. The corresponding fluorescence was recorded from the surfaces of intact apple fruits. It appears that the fluorescence analysis of farnesene oxidation products is a useful, specific, and sensitive tool to follow their concentrations both in extracts and intact fruits.


2000 ◽  
Vol 104 (28) ◽  
pp. 6566-6572 ◽  
Author(s):  
Lorenzo Catani ◽  
Cristina Gellini ◽  
Laura Moroni ◽  
Pier Remigio Salvi

1988 ◽  
Vol 66 (8) ◽  
pp. 721-723
Author(s):  
G. Gouédard ◽  
N. Billy ◽  
B. Girard ◽  
J. Vigué

We have studied the fluorescence excitation spectrum of natural Cl2 molecules induced by a monomode ring dye laser around various X(ν″ = 0) → B(ν′ ≥ 18) bandheads. Contrary to the main 35–35Cl2 isotope, the less abundant species 35–37Cl2 and 37–37Cl2 have not, up to now, been thoroughly investigated in this region. Our experiment has allowed a precise determination of a number of isotope shifts, which are shown to be calculable by a local interpolation procedure of Coxon's data, using the concept of mass-reduced quantum numbers.


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