Experimental Laparoscopic Hepatectomy of Left Lateral Lobe in Pig Models

The present report describes a novel approach to and presents the surgical results for laparoscopic hepatectomy of the left lobes in small dogs. A purely laparoscopic four-port approach was used in seven beagles. The left triangular ligament was cut first, then the base of the left medial lobe (LMB) was ligated with silk thread using a needle, and the liver parenchyma of the LMB approximately 0.5 cm above the ligation site was removed with LigaSure. The left lateral lobe (LLB) was removed in the same manner. Blood was collected on postoperative days 1, 3, 7, and 14 for laboratory testing. Left hepatic lobe resection was successfully completed in all dogs, with an average operation time of 102.53 ± 9.07 min and an average blood loss of 32.10 ± 6.43 ml. Serious postoperative complications were not observed. The white blood cell (WBC) count and alkaline phosphatase (ALP) level were significantly increased in experimental dogs 1 day after surgery (P < 0.05); aspartate transferase (AST) and alanine transaminase (ALT) were significantly different before and after surgery (P < 0.01); and all the indicators were basically normal seven days after the operation. Exploratory laparoscopy at 28 days revealed adhesion of the surface of the liver section to the omentum or the gastric wall, but there were no other abnormal findings. The combined application of liver pedicle ligation and LigaSure for liver lobectomy provides a simple and effective method for generating liver lobectomy models for research on liver disease and regeneration and a feasible laparoscopic liver lobectomy operation scheme for small dogs (10–15 kg).

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Complex outflow anatomy in left lateral lobe graft and modified venous reconstruction in pediatric living donor liver transplantation

Transplant International ◽

10.1111/tri.12388 ◽

2014 ◽

Vol 27 (10) ◽

pp. e105-e108 ◽

Cited By ~ 2

Author(s):

Nikolaos Benetatos ◽

Irene Scalera ◽

John R. Isaac ◽

Darius F. Mirza ◽

Paolo Muiesan

Keyword(s):

Liver Transplantation ◽

Living Donor ◽

Living Donor Liver Transplantation ◽

Lateral Lobe ◽

Donor Liver ◽

Venous Reconstruction ◽

Donor Liver Transplantation ◽

Left Lateral Lobe

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Phenobarbital-induced hepatocellular proliferation: anti-bromodeoxyuridine and anti-proliferating cell nuclear antigen immunocytochemistry.

Journal of Histochemistry & Cytochemistry ◽

10.1177/41.1.8093255 ◽

1993 ◽

Vol 41 (1) ◽

pp. 21-27 ◽

Cited By ~ 32

Author(s):

H B Jones ◽

N A Clarke ◽

N C Barrass

Keyword(s):

Cell Proliferation ◽

Proliferating Cell Nuclear Antigen ◽

Lateral Lobe ◽

Nuclear Antigen ◽

Brdu Incorporation ◽

Advantages And Disadvantages ◽

Immunocytochemical Detection ◽

Left And Right ◽

Proliferating Cell ◽

Left Lateral Lobe

We report modifications to immunocytochemical detection procedures for proliferating cell nuclear antigen (PCNA) which permit its identification in liver samples previously fixed for BrdU immunocytochemistry. Both methods have been used for the assessment of phenobarbital-induced cell proliferation in rat liver. The difficulties associated with the hitherto unsuccessful application of PCNA immunocytochemical methods to tissues fixed in formalin for BrdU visualization were overcome by epitope unmasking with acid hydrolysis, extension of primary antiserum (PC10) incubation, and employment of streptavidin-ABC-HRP. BrdU delivery via osmotic minipumps for 48 hr before euthanasia, followed by fixation in cold formalin for 14 days, yielded reliable and reproducible hepatocellular labeling and a peak of cell proliferation in all lobes on Day 3 (i.e., labeling during Days 1-3) of dosing with 80 mg/kg/day phenobarbital. Labeling indices (LI) of both control and phenobarbital-treated liver were lower in the left and right median lobes as compared with the lateral lobes. In sections of the left lateral lobe from the same liver, PCNA immunocytochemistry revealed a peak of proliferative activity (about one third of the maximum LI generated by BrdU incorporation) on Day 1. These findings, together with the advantages and disadvantages of both techniques, are discussed in the context of their applications to different investigative requirements.

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Determining Transgene Expression Characteristics Using a Suction Device with Multiple Hole Adjusting a Left Lateral Lobe of the Mouse Liver

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b18-00094 ◽

2018 ◽

Vol 41 (6) ◽

pp. 944-950 ◽

Cited By ~ 4

Author(s):

Ayana Haraguchi ◽

Yuki Fuchigami ◽

Maho Kawaguchi ◽

Shintaro Fumoto ◽

Kaname Ohyama ◽

...

Keyword(s):

Mouse Liver ◽

Transgene Expression ◽

Lateral Lobe ◽

Suction Device ◽

Expression Characteristics ◽

Left Lateral Lobe

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Thoracoschisis secondary to a mesenchymal hamartoma associated with diaphragmatic eventration

Case Reports in Perinatal Medicine ◽

10.1515/crpm-2015-0081 ◽

2016 ◽

Vol 5 (2) ◽

pp. 141-144 ◽

Cited By ~ 2

Author(s):

Colm P. Travers ◽

Jared Austin Hamm ◽

Sue Cleveland ◽

Mike K. Chen ◽

Scott Anderson ◽

...

Keyword(s):

Congenital Anomaly ◽

Chest Wall ◽

Diaphragmatic Hernia ◽

Body Wall ◽

Lateral Lobe ◽

Pathological Examination ◽

Mesenchymal Hamartoma ◽

Wall Defect ◽

Rare Congenital Anomaly ◽

Left Lateral Lobe

Abstract Thoracoschisis is an extremely rare congenital anomaly associated with limb body wall defect and diaphragmatic hernia. We describe a case of a female infant who was noted at birth to have tissue coming through a left sided thoracic defect next to an accessory nipple. The stomach bubble was displaced superiorly on radiographs. At surgery the tissue was attached to the left lateral lobe of the liver and was protruding through the chest wall via an intercostal defect below an eventrated diaphragm. The tissue was resected and the defect closed. Pathological examination was consistent with a mesenchymal hamartoma. The diaphragm may have formed abnormally in this case due to the presence of the mesenchymal hamartoma in this location.

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