Protective role of Eclipta alba L. extract against ethinylestradiol induced genotoxic damage in cultured human lymphocytes

In India, natural preparations derived from plants are widely used for the treatment of various diseases. Hence it becomes necessary to assess the modulating action of the plant extracts when associated with other substances. Ethinylestradiol is not only a genotoxic agent but also a tumor initiating agent. It is widely used in oral contraceptive formulations and also for the treatment of various sexual and metabolic disorders. In the present study, the antigenotoxic effect of Eclipta alba was evaluated against the genotoxic effect induced by 10 μM of ethinylestradiol in the presence of metabolic activation using mitotic index (MI), chromosomal aberrations, sister chromatid exchanges and replication index (RI) as parameters. The treatment of 10 μM of ethinylestradiol along with 1.02x10–4, 2.125x10–4, 3.15x10–4 and 4.17x10–4 g/mL of Eclipta alba (E. alba) extract in culture medium results in a significant dose dependent decrease in the genotoxic effects induced by the treatment of 10 μM of ethinylestradiol. The results of the present study suggest that the plant extract per se does not have genotoxic potential, but can modulate the genotoxicity of ethinylestradiol in cultured human lymphocytes.

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Additive Action of Vitamins C and E against Hydrocortisone-Induced Genotoxicity in Human Lymphocyte Chromosomes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831.72.4.204 ◽

2002 ◽

Vol 72 (4) ◽

pp. 204-209 ◽

Cited By ~ 10

Author(s):

Sultan Ahmad ◽

Afsahul Hoda ◽

Mohammad Afzal

Keyword(s):

Chromosomal Aberrations ◽

Human Lymphocyte ◽

Sister Chromatid ◽

Human Lymphocytes ◽

Protective Role ◽

Sister Chromatid Exchanges ◽

Additive Action ◽

Hydrocortisone Administration ◽

Chromatid Exchanges

In earlier reports, hydrocortisone administration to human lymphocytes in culture was shown to cause chromosomal aberrations and increased sister chromatid exchanges. With a view to study the ameliorative action of some antioxidants against this effect, vitamins C and E were used separately and in combination along with hydrocortisone treatment, at different dosage and for different durations, on human lymphocyte cultures. The levels of chromosomal aberrations and sister chromatid exchanges were lowered, suggesting a protective role of vitamins against genotoxic damage. Administration of vitamins C and E combined appeared to be more effective in preventing chromosomal damage than separate administration, demonstrating the additive action of these vitamins against steroid-induced genotoxicity.

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Evaluation of Possible Genotoxic Activity of Dirithromycin in Cultured Human Lymphocytes

Journal of Toxicology ◽

10.1155/2015/535490 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Ahmet Kayraldız ◽

Lale Dönbak ◽

Ayşe Yavuz Kocaman ◽

Esra Köker ◽

Şule Gökçe

Keyword(s):

Bacterial Infections ◽

Human Lymphocytes ◽

Sister Chromatid Exchanges ◽

Mutagenic Action ◽

Replication Index ◽

Different Types ◽

Nuclear Division Index ◽

Almost All ◽

Chromatid Exchanges

Dirithromycin antibiotic is a 14-membered lactone ring macrolide and is widely used in medicine to treat many different types of bacterial infections. In the present study, the possible genotoxicity of dirithromycin was evaluated in cultured human lymphocytes by using sister chromatid exchanges (SCEs), chromosome aberration (CA), and micronucleus (MN) tests and also cell proliferation kinetics such as mitotic index (MI), replication index (RI), and nuclear division index (NDI) were analyzed for cytotoxicity. Cell cultures were treated with four different concentrations of dirithromycin (37.75, 67.50, 125, and 250 µg/mL) for 24 and 48 h periods. Dirithromycin significantly induced SCE and MN frequency at all concentrations in both 24 and 48 h treated cells. In addition, CA level has been markedly increased in the cells treated with almost all concentrations of dirithromycin for 24 (except 37.75 µg/mL) and 48 h treatment periods as compared to control. However, MI, RI, and NDI values were not affected by the dirithromycin treatment (p> 0.05). The results of this study indicated that dirithromycin treatment caused genetic damage by increasing the level of cytogenetic endpoints, suggesting its genotoxic and mutagenic action on human lymphocytesin vitro.

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Induction of sister-chromatid exchanges in human lymphocytes by indirect carcinogens with and without metabolic activation

Mutation Research/Genetic Toxicology ◽

10.1016/0165-1218(83)90129-5 ◽

1983 ◽

Vol 117 (3-4) ◽

pp. 301-309 ◽

Cited By ~ 12

Author(s):

Kiyoshi Inoue ◽

Tadayoshi Shibata ◽

Tatsuo Abe

Keyword(s):

Sister Chromatid ◽

Human Lymphocytes ◽

Metabolic Activation ◽

Sister Chromatid Exchanges ◽

Chromatid Exchanges

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The protective role of ascorbic acid on imazalil-induced genetic damage assessed by the cytogenetic tests

Toxicology and Industrial Health ◽

10.1177/0748233711420471 ◽

2011 ◽

Vol 28 (7) ◽

pp. 648-654 ◽

Cited By ~ 13

Author(s):

Hasan Türkez ◽

Elanur Aydın

Keyword(s):

Ascorbic Acid ◽

Human Lymphocytes ◽

Protective Role ◽

Genotoxic Damage ◽

Metabolic Functions ◽

Human Peripheral Lymphocytes ◽

Dose Dependent Decrease ◽

First Time

Ascorbic acid (AA), known as vitamin C, has important antioxidant and metabolic functions, making its incorporation into the human diet essential. On the other hand, imazalil (IMA), a commonly used fungicide in both agricultural and clinical domains is suspected to produce very serious toxic effects in vertebrates. In this study, the antigenotoxic effects of AA were studied against the genotoxic damage induced by IMA on cultured human lymphocytes using chromosomal aberration (CA) and sister chromatid exchange (SCE) as genetic end points. Human peripheral lymphocytes were treated in vitro with varying concentrations of AA (25, 50, 100, 200, and 400 μg/ml), tested in combination with IMA (336 mg/L). AA alone was not genotoxic and when combined with IMA treatment, reduced the frequencies of CAs and SCEs. A clear dose-dependent decrease in the genotoxic damage of IMA was observed, suggesting a genoprotective role of AA. In conclusion, the preventive role of AA in alleviating IMA-induced DNA damage was indicated for the first time in the present study.

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