scholarly journals A study on the prevalence of dog erythrocyte antigen 1.1 and detection of canine Babesia by polymerase chain reaction from apparently healthy dogs in a selected rural community in Zimbabwe

2016 ◽  
Vol 87 (1) ◽  
Author(s):  
Solomon Dhliwayo ◽  
Tariro A. Makonese ◽  
Belinda Whittall ◽  
Silvester M. Chikerema ◽  
Davies M. Pfukenyi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Group ◽  
Blood Type ◽  
Chain Reaction ◽  
Blood Typing ◽  
Life Threatening ◽  
Polymerase Chain ◽  
Erythrocyte Antigen ◽  
Babesia Spp ◽  
Dog Erythrocyte

A study was carried out to determine the prevalence of blood group antigen dog erythrocyte antigen (DEA) 1.1 in mixed breed dogs in rural Chinamhora, Zimbabwe. DEA 1.1 is clinically the most important canine blood group as it is the most antigenic blood type; hence, DEA 1.1 antibodies are capable of causing acute haemolytic, potentially life-threatening transfusion reactions. In this study, blood samples were collected from 100 dogs in Chinamhora, and blood typing was carried out using standardised DEA 1.1 typing strips with monoclonal anti–DEA 1.1 antibodies (Alvedia® LAB DEA 1.1 test kits). Polymerase chain reaction for detecting Babesia spp. antigen was carried out on 58 of the samples. Of the 100 dogs, 78% were DEA 1.1 positive and 22% were DEA 1.1 negative. A significantly (p = 0.02) higher proportion of females (90.5%) were DEA 1.1 positive than males (69.0%). The probability of sensitisation of recipient dogs following first-time transfusion of untyped or unmatched blood was 17.2%, and an approximately 3% (2.95%) probability of an acute haemolytic reaction following a second incompatible transfusion was found. Babesia spp. antigen was found in 6.9% of the samples. No significant relationship (χ2 = 0.56, p = 0.45) was found between DEA 1.1 positivity and Babesia spp. antigen presence. Despite a low probability of haemolysis after a second incompatibility transfusion, the risk remains present and should not be ignored. Hence, where possible, blood typing for DEA 1.1 is recommended. A survey of DEA 3, 4, 5 and 7 in various breeds is also recommended.

scholarly journals Frequency of dog erythrocyte antigen 1 blood group and risk of incompatible transfusion in dogs of different breeds and mongrels from the city of Salvador - BA, Brazil

2019 ◽  
Vol 56 (3) ◽  
pp. e154865
Author(s):  
Suzana Cláudia Spínola dos Santos ◽  
Mariane Melo dos Santos ◽  
Wellington Francisco Rodrigues ◽  
Roberto Meyer ◽  
Maria de Fátima Dias Costa
Keyword(s):  
Flow Cytometry ◽  
Blood Group ◽  
Blood Type ◽  
Blood Transfusions ◽  
Blood Typing ◽  
Reaction Test ◽  
Erythrocyte Antigen ◽  
The City ◽  
Dog Erythrocyte ◽  
Group 1

The dog erythrocyte antigen 1 (DEA 1) is the most immunogenic blood group in dogs, and blood transfusions may trigger some undesirable effects in veterinary patients, which are directly associated with incompatible transfusions. The present study aimed to investigate the frequency of positive DEA 1 blood group in blood donor dogs from a blood bank in Salvador, Bahia, Brazil, and also to calculate the risk of managing incompatible blood in both first and second transfusion. A number of 203 dogs of different breeds, aged between 1 and 8 years, weighing 28 kg, with no degree of kinship and of both sexes in Salvador - BA, Brazil were evaluated to investigate the blood type DEA 1 frequency, by means of chromatography and flow cytometry tests for blood typing. The risk of incompatible blood transfusion in either a first or a second transfusion was also calculated. The frequency of the DEA 1 group ranged from 0% to 100% in various breeds, but with a mean positivity of 62.07% (126/203). And the lowest risk of an DEA 1 negative animal receiving DEA 1 positive blood within the group of animals evaluated was 0.92% at a first transfusion; and the risk of the same animal receiving incompatible blood for the DEA group 1 in the second transfusion was 0.008%. The highest risk of an DEA 1 negative animal receiving DEA 1 positive blood from these animals was 69.12%; and the risk of receiving incompatible blood for DEA 1 was 47.77%. In conclusion, the frequency of the DEA 1 group varied between the studied breeds and the risk of incompatible blood transfusions varies according to donor and recipiente breeds, but this can be overridden if blood typing tests are performed along with the cross-reaction test for compatibility.

scholarly journals Rapid genotyping of the major alleles at the Duffy (FY) blood group locus using real-time fluorescence polymerase chain reaction

2020 ◽  
Vol 17 (2) ◽  
pp. 42-44
Author(s):  
Fernando M. Araújo ◽  
Christina Pereira ◽  
Ana Aleixo ◽  
Isabel Henriques ◽  
Fátima Monteiro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Blood Group ◽  
Chain Reaction ◽  
Group Locus ◽  
Polymerase Chain

scholarly journals Rapid Rh D genotyping by polymerase chain reaction-based amplification of DNA

Blood ◽  
1995 ◽  
Vol 85 (10) ◽  
pp. 2975-2980 ◽  
Author(s):  
S Simsek ◽  
BH Faas ◽  
PM Bleeker ◽  
MA Overbeeke ◽  
HT Cuijpers ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Group ◽  
False Positive ◽  
Complete Agreement ◽  
Blood Group System ◽  
Chain Reaction ◽  
Group System ◽  
Allele Specific ◽  
Polymerase Chain ◽  
Rh Blood Group

Rh (rhesus) D is the dominant antigen of the Rh blood group system. Recent advances in characterization of the nucleotide sequence of the cDNA(s) encoding the Rh D polypeptide allow the determination of the Rh D genotype at the DNA level. This can be of help in cases in which red blood cells are not available for phenotyping, eg, when in concerns a fetus. We have tested three independent DNA typing methods based on the polymerase chain reaction (PCR) for their suitability to determine the Rh D genotype. DNA derived from peripheral blood mononuclear cells from 234 Rh-phenotyped healthy donors (178 Rh D positive and 56 Rh D negative) was used in the PCR. The Rh D genotypes, as determined with a method based on the allele-specific amplification of the 3′ noncoding region of the Rh D gene described by Bennett et al (N Engl J Med 329:607, 1993), were not concordant with the serologically established phenotypes in all cases. We have encountered 5 discrepant results, ie, 3 false-positive and 2 false-negative (a father and child). Rh D genotyping with the second method was performed by PCR amplification of exon 7 of the D gene with allele-specific primers. In all donors phenotyped as D positive tested so far (n = 178), the results of molecular genotyping with this method were concordant with the serologic results, whereas a false-positive result was obtained in one of the D-negative donors (also false-positive in the first method). Complete agreement was found between genotypes determined in the third method, based on a 600-bp deletion in intron 4 of the Rh D gene described by Arce et al (Blood 82:651, 1993), and serologically determined phenotypes. The Rh blood group system is complex, and unknown polymorphisms at the DNA level are expected to exist. Therefore, although genotypes determined by the method of Arce et al were in agreement with serotypes, it cannot yet be regarded as the golden standard. More experience with this or other methods is still needed.

scholarly journals The breed prevalence of Dog Erythrocyte Antigen 1.1 in the Onderstepoort area of South Africa and its significance in selection of canine blood donors

2002 ◽  
Vol 73 (2) ◽  
Author(s):  
L.L. Van der Merwe ◽  
L.S. Jacobson ◽  
J.G. Pretorius
Keyword(s):  
South Africa ◽  
Blood Group ◽  
Blood Donors ◽  
German Shepherd ◽  
Animal Blood ◽  
Breed Differences ◽  
Life Threatening ◽  
Erythrocyte Antigen ◽  
N 93 ◽  
Dog Erythrocyte

The blood group antigen Dog Erythrocyte Antigen (DEA) 1.1 is clinically the most important canine blood group as DEA 1.1 antibodies are capable of causing acute haemolytic, potentially life-threatening transfusion reactions. Dogs do not have naturally occurring antibodies to DEA 1.1 but are rapidly sensitised by the first incompatible transfusion. The prevalence of DEA 1.1 in the general dog population is estimated at 42-46 %. Canine blood donors registered with the Onderstepoort Animal Blood Bank (n = 93) as well as potential donors (n = 140) were typed for DEA 1.1 using a monoclonal antibody card kit. All dogs came from the Onderstepoort area, near Pretoria, Gauteng province, South Africa. Overall prevalence of DEA 1.1 was 47 %. Prevalence was 47 % in purebred dogs and 48 % in mongrels. Distinct breed differences were noted with less than 20 % of German shepherd dogs and Boxers and greater than 75 % of Rottweilers, Great Danes, St Bernards and Dalmations testing DEA 1.1 positive. Knowledge of local breed differences will increase effectiveness of blood donor recruitment.

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