scholarly journals Study of polarization colors in the connective tissue wall of odontogenic cysts using picrosirius red stain

2015 ◽  
Vol 7 (2) ◽  
pp. 119 ◽  
Author(s):  
Sandhya Tamgadge ◽  
Anusha Shetty ◽  
Avinash Tamgadge ◽  
Sudhir Bhalerao ◽  
Treville Periera ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Odontogenic Cysts ◽  
Picrosirius Red

scholarly journals Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

2017 ◽  
Vol 6 (2) ◽  
pp. 1518
Author(s):  
Parvez Ikram ◽  
Nadeem Jeddy
Keyword(s):  
Connective Tissue ◽  
Large Scale ◽  
Oral Submucous Fibrosis ◽  
Muscle Layer ◽  
Inclusion Criteria ◽  
Polarizing Microscopy ◽  
Potentially Malignant ◽  
Tissue Changes ◽  
Submucous Fibrosis ◽  
Picrosirius Red

<p><strong>Background:</strong> Oral submucous fibrosis (OSMF), a potentially malignant oral disorder has the highest rate of malignant transformation of about 7-13%. The connective tissue changes that occur in this disease are characteristic and are stained with special stains.</p><p><strong>Objective:</strong> The study was done to compare common and special stains under light microscopy and polarizing microscopy to evaluate the levels of fibrosis in oral submucous fibrosis and assess the type of collagen present in the stromal area.</p><p><strong>Materials and Methods:</strong> Fifty tissue blocks were selected from the archives and were prepared and stained with H&amp;E, Masson's trichrome, Van Gieson and Picrosirius red and studied under light microscope and polarizing microscope respectively.</p><p><strong>Results:</strong> H and E stained slides were useful in diagnosing the lesion but was not able to highlight the level of fibrosis. Masson's trichrome and Van Gieson stained slides showed the depth of the lesion which extended even to the deeper muscle layer. The type of collagen present was definitively seen by the birefringence in polarizing microscopic study. Interobserver variation was less and all the values regarding the effectiveness of the special stains in detecting the level of fibrosis were statistically significant.</p><p><strong>Conclusion:</strong> Special stains can be used routinely in laboratories to demonstrate connective tissue lesions especially in cases of OSMF. Depth of the lesion and the area of involvement help in treatment planning to be delivered. Large scale studies with more categories and inclusion criteria are required along with the special stains to assess the other alterations in OSMF.</p>

Hyaline bodies in the connective tissue wall of odontogenic cysts

1981 ◽  
Vol 10 (3) ◽  
pp. 147-157 ◽  
Author(s):  
Sow-Yen Chen ◽  
John E. Fantasia ◽  
Arthur S. Miller
Keyword(s):  
Connective Tissue ◽  
Odontogenic Cysts

scholarly journals Collagen and Its Role in predicting the Biological Behavior of Odontogenic Lesions

2017 ◽  
Vol 18 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Pavan G Kulkarni ◽  
M Aruna Kumari ◽  
Abhishek Jahagirdar ◽  
SRK Nandan ◽  
Shyam Prasad Reddy D ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Collagen Fibers ◽  
Biological Behavior ◽  
Polarization Microscopy ◽  
Keratocystic Odontogenic Tumor ◽  
Ameloblastic Carcinoma ◽  
Greenish Yellow ◽  
Unicystic Ameloblastoma ◽  
Dentigerous Cysts ◽  
Picrosirius Red

ABSTRACT Introduction Odontogenic cysts and tumors have variable recurrence rates. Recurrence rate is mainly due to the activity of the epithelium. The epithelium of these lesions has been investigated extensively in regard to their role in proliferative and aggressive behavior of the lesions. However, the role of the connective tissue wall in their behavior has not been studied as extensively. Collagen is an essential part of the connective tissue as a whole and fibrous wall of cystic lesions especially. It is demonstrated by picrosirius red dye staining combined with polarization microscopy. This method permits the evaluation of the nature of the collagen fibers in addition to their thickness. Materials and methods A total of 56 histopathologically diagnosed cases comprising odontogenic follicle, dentigerous cyst, unicystic ameloblastoma, keratocystic odontogenic tumor (KCOT), multicystic/solid ameloblastoma, and ameloblastic carcinoma were taken and stained using picrosirius red stain and evaluated using a polarizing microscope. Results Collagen fibers in odontogenic follicles and dentigerous cysts showed predominant orange-red birefringence; fibers in unicystic ameloblastoma and KCOT showed both orange red and greenish-yellow birefringence; and fibers of multicystic/ solid ameloblastoma showed predominant greenish-yellow birefringence and ameloblastic carcinoma that showed almost complete greenish birefringence. As the biological behavior of the lesions in the spectrum studied progress toward aggressive nature, increase in immature collagen fibers is noticed. Conclusion This study suggests that the nature of collagen fibers plays a pivotal role in predicting the biological behavior of odontogenic lesions. Clinical significance Aggressive nature of the odontogenic lesions is determined by both the epithelium and the connective tissue components (collagen). Studying the nature and type of collagen helps in predicting its biological behavior. How to cite this article Kulkarni PG, Kumari MA, Jahagirdar A, Nandan SRK, Reddy DSP, Keerthi M. Collagen and Its Role in predicting the Biological Behavior of Odontogenic Lesions. J Contemp Dent Pract 2017;18(2):137-141.

scholarly journals Post-radiation changes in oral tissues - An analysis of cancer irradiation cases

2014 ◽  
Vol 03 (03) ◽  
pp. 159-162 ◽  
Author(s):  
Jay Ashokkumar Pandya ◽  
N. Srikant ◽  
Karen Boaz ◽  
Nidhi Manaktala ◽  
Supriya Nikita Kapila ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Tumor Stroma ◽  
Wall Thickening ◽  
Chi Square ◽  
True Nature ◽  
History Of ◽  
Post Radiation ◽  
Pattern Of Invasion ◽  
Picrosirius Red

Abstract Introduction: Radiation, commonly employed as neoadjuvant, primary, and adjuvant therapy for head and neck cancer causes numerous epithelial and stromal changes, prominent among which is fibrosis with its early and late consequences. Very little is known about the true nature of the fibrosed tissue and the type of fibers accumulated. Radiotherapy affects the supporting tumor stroma often resulting in a worsening grade of tumor post-radiation. Aim: To study epithelial, neoplastic, stromal, and glandular changes in oral cavity induced by radiation therapy for oral squamous cell carcinoma (OSCC) using special stains. Materials and Methods: The study included 27 samples of recurrent OSCC following completion of radiotherapy (recurrence within an average span of 11 months), and 26 non-irradiated cases of OSCC. Patients with a history of combined radiotherapy and chemotherapy were not included in the study. The epithelial changes assessed included epithelial atrophy, apoptosis, necrosis, dysplasia, and neoplasia. The connective tissue was evaluated for amount of fibrosis, quality of fibers (using picrosirius red staining), fibrinous exudate, necrosis, pattern of invasion, vessel wall thickening, and salivary gland changes. The aforementioned changes were assessed using light and polarizing microscopy and tabulated. Statistical Analysis: Epithelial and connective tissue parameters were compared between the irradiated and non-irradiated cases using chi square and t-tests. Results: Epithelial and connective tissue parameters were found to be increased in irradiated patients. Pattern of invasion by tumor cells varied from strands and  cords between the two groups studied. The effect of radiation was seen to reflect on the maturity of fibers and the regularity of their distribution.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Sign in / Sign up

Export Citation Format

Share Document