scholarly journals Biofilm forming capacity of Enterococcus faecalis on Gutta-percha points treated with four disinfectants using confocal scanning laser microscope: An in vitro study

2015 ◽  
Vol 12 (4) ◽  
pp. 331 ◽  
Author(s):  
PolavarapuVenkata Ravi Chandra ◽  
VemisettyHari Kumar ◽  
SurakantiJayaprada Reddy ◽  
DandoluRam Kiran ◽  
MuppalaNagendra Krishna ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscope ◽  
Gutta Percha ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

1989 ◽  
Vol 47 ◽  
pp. 146-147
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Pyramidal Neurons ◽  
Light And Electron Microscopy ◽  
Scanning Laser ◽  
Laser Exposure ◽  
Confocal Scanning Laser Microscope ◽  
Fluorescent Markers ◽  
Before And After ◽  
Staining Protocol ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

An in-vitro study on the effectiveness of different irrigation solutions against Enterococcus faecalis with/without Erbium laser activation

2020 ◽  
Vol 2 (4) ◽  
pp. 1-1
Author(s):  
Gülşah Balan ◽  
Sadık Kalaycı ◽  
Ayşen Yarat ◽  
Serap Akyüz ◽  
Firettin Şahin
Keyword(s):  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Erbium Laser

An In Vitro Study Comparing the Intracanal Effectiveness of Calcium Hydroxide– and Linezolid-based Medicaments against Enterococcus faecalis

2012 ◽  
Vol 38 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Rajdeep Pavaskar ◽  
Ida de Noronha de Ataide ◽  
Paul Chalakkal ◽  
Maria J. Pinto ◽  
Kristlee Sabrin Fernandes ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study

EVALUATION OF THE DISSOLVING EFFICACY OF ESSENTIAL OILS ON ENDODONTIC OBTURATING MATERIALS: AN IN VITRO STUDY.

2021 ◽  
pp. 66-68
Author(s):  
Rakesh Pyla ◽  
Jai Kiran Killada ◽  
V Raja Sekhar
Keyword(s):  
Essential Oils ◽  
Epoxy Resin ◽  
In Vitro Study ◽  
Vitro Study ◽  
Lavender Oil ◽  
Citronella Oil ◽  
Gutta Percha ◽  
Lemon Oil ◽  
The Difference

Aim:Endodontic retreatment is a procedure that removes the lling materials from the root canals followed by their cleaning, shaping and obturation. This in-vitro study aimed to compare and evaluate the ability of various essential oils as solvents in dissolving gutta-percha, epoxy resin, and zinc oxide eugenol (ZOE) cements. Materials and methods: A total of 28 cylindrical specimens in each group ZOE, epoxy resin, 28 ISO size 40 gutta-percha cones were prepared and divided into four groups for immersion in the different solvents, i.e. lemon oil, citronella oil, lavender oil, and TCE(Tetrachloroethylene (control)) for 5 minutes. The obturating materials dissolution in the solvents were obtained by the difference between the pre-immersion original weight and the post-immersion weight on a digital analytical scale. Data were statistically analysed by a paired t-test and Analysis of Variance (ANOVA) (p < 0.05). Results: Order of efcacy of dissolution of essential oils was found to be lemon oil > Citronella oil > Lavender oil and was highly signicant (p < 0.01). In all the solvents, Gutta-percha showed maximum dissolution (Fvalue:149.56) followed by ZOE(89.07 ) and resin sealer least (23.86). Conclusion: It can be concluded that the lemon oil can be used as a solvent for dissolving obturating materials.

Diagnostic Revolution of Microhematuria by Real Time Confocal Scanning Laser Microscope: Hyodo-Iino-Miyagawa Method, Third Report

Nephron ◽  
1995 ◽  
Vol 70 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Toru Hyodo ◽  
Ikuo Miyagawa ◽  
Akihiro Iino ◽  
Koji Ono ◽  
Tsutomu Kuomi ◽  
...  
Keyword(s):  
Real Time ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscope ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

scholarly journals Antibacterial efficacy of biosynthesized silver nanoparticles against Enterococcus faecalis Biofilm: An in vitro study

2018 ◽  
Vol 9 (2) ◽  
pp. 237 ◽  
Author(s):  
KiranRahul Halkai ◽  
JayashreeA Mudda ◽  
Vasundhara Shivanna ◽  
Vandana Rathod ◽  
Rahul Halkai
Keyword(s):  
Silver Nanoparticles ◽  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibacterial Efficacy
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