Evaluation of histological and pH changes in platelet-rich fibrin and platelet-rich fibrin matrix: A In vitro study

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

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Comparative evaluation of platelet count and antimicrobial efficacy of injectable platelet-rich fibrin with other platelet concentrates: An in vitro study

Journal of Indian Society of Periodontology ◽

10.4103/jisp.jisp_201_17 ◽

2017 ◽

Vol 21 (2) ◽

pp. 97 ◽

Cited By ~ 5

Author(s):

PrernaAshok Karde ◽

KunalSunder Sethi ◽

SwapnaArunkumar Mahale ◽

SmitaUday Khedkar ◽

AgrajaGanpat Patil ◽

...

Keyword(s):

Platelet Count ◽

Comparative Evaluation ◽

In Vitro Study ◽

Vitro Study ◽

Antimicrobial Efficacy ◽

Platelet Concentrates ◽

Platelet Rich Fibrin

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Comparative evaluation of platelet-rich fibrin, platelet-rich fibrin + 50 wt% nanohydroxyapatite, platelet-rich fibrin + 50 wt% dentin chips on odontoblastic differentiation - An in vitro study-part 2

Journal of Conservative Dentistry ◽

10.4103/jcd.jcd_3_20 ◽

2020 ◽

Vol 23 (4) ◽

pp. 354

Author(s):

Kottuppallil Girija ◽

Mahendran Kavitha

Keyword(s):

Comparative Evaluation ◽

In Vitro Study ◽

Vitro Study ◽

Platelet Rich Fibrin ◽

Odontoblastic Differentiation

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Drug Delivery Assessment of a Novel Triple Antibiotic-Eluting Injectable Platelet-Rich Fibrin Scaffold: An in-vitro Study.

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200605110250 ◽

2020 ◽

Vol 21 ◽

Cited By ~ 1

Author(s):

Azade Rafiee ◽

Mahtab Memarpour ◽

Sara Taghvamanesh ◽

Forough Karami ◽

Somayeh Karami ◽

...

Keyword(s):

High Performance ◽

In Vitro Study ◽

Vitro Study ◽

Burst Release ◽

Platelet Rich Fibrin ◽

Fibrin Scaffold ◽

Antibiotic Release ◽

Simultaneous Identification

Background: Intracanal disinfection is a critical, yet challenging goal for the long-term success in regenerative-based treatments. This in-vitro study aimed to assess the release profile of triple antibiotic-eluting injectable platelet-rich fibrin (I-PRF) constructs in 28 days. Methods: I-PRF scaffolds containing triple antibiotic mixture [metronidazole (MET), ciprofloxacin (CIP), and minocycline (MINO)] by immersion (group one), I-PRF scaffolds containing triple antibiotic mixture by integration (group two), and antibiotic-free I-PRF scaffolds (group three) were fabricated. The antibiotic release from the scaffolds was measured using a high performance liquid chromatography (HPLC) (the mobile phase of 0.1% formic acid and methanol (35:65 v/v), a C18 analytical column (150 × 4.6 mm, 5 μm) at a flow rate of 0.7 mL/min, at 25ºC) at days 1, 3, 7, 14, 21, and 28. Results: Retention times for MINO, CIP, and MET were achieved as 2.3, 2.6, and 3.1 min, respectively. The maximum UV absorbances for CIP, MET, and MINO were at 268 nm, 278 nm, and 350 nm, respectively. The results of the first group showed burst release within the first 24 hours followed by sustained maintenance of all three antibiotics up to 14 days. MINO and MET were still detectable in the third week. The second group could not sustainably release of the antibiotics. Conclusions: The developed method for the simultaneous identification, and quantification of each antibiotic in I-PRF was sensitive and quick. Overall, group one could take up the antibiotics in adequate quantities and then subsequently release them over the study period.

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Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study

BioMed Research International ◽

10.1155/2018/9031435 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Marco Lollobrigida ◽

Manuela Maritato ◽

Giuseppina Bozzuto ◽

Giuseppe Formisano ◽

Agnese Molinari ◽

...

Keyword(s):

Autologous Blood ◽

Combined Treatment ◽

Pure Titanium ◽

In Vitro Study ◽

Vitro Study ◽

Adjunctive Treatment ◽

Machined Surface ◽

Implant Surface ◽

Platelet Rich Fibrin

Objective. Platelet-rich fibrin (PRF) clots and membranes are autologous blood concentrates widely used in oral surgical procedures; less is known, however, about the liquid formulations of such products. The aim of this in vitro study is to assess the behavior of different implant surfaces when in contact with two liquid leucocyte- and platelet-rich fibrin (L-PRF) products.Methods. Six commercial pure titanium discs, of 9.5 mm diameter and 1.5 mm thickness, were used. Three of these samples had a micro/nano-rough surface; three were machined. Three different protocols were tested. Protocols involved the immersion of the samples in(1)a platelets, lymphocytes, and fibrinogen liquid concentrate (PLyF) for 10 minutes,(2)an exudate obtained from L-PRF clots rich in fibronectin and vitronectin for 5 minutes, and(3)the fibronectin/vitronectin exudate for 2 minutes followed by immersion in the PLyF concentrate for further 8 minutes. After these treatments, the samples were fixed and observed using a scanning electron microscope (SEM).Results. Under microscopic observation,(1)the samples treated with the PLyF concentrate revealed a dense fibrin network in direct contact with the implant surface and a significant number of formed elements of blood;(2)in the samples treated with the fibronectin/vitronectin exudates, only a small number of white and red blood cells were detectable; and(3)in samples exposed to the combined treatment, there was an apparent increase in the thickness of the fibrin layer. When compared to the machined surface, the micro/nano-rough samples showed an overall increased retention of fibrin, leading to a thicker coating.Conclusions. Liquid L-PRF products promote the formation of a dense fibrin clot on micro/nano-rough implant surfaces in vitro. The adjunctive treatment of surfaces with the fibronectin/vitronectin exudate could provide support to contact of the fibrin with the surface, though it is not essential for the clot formation. Further studies are necessary to better elucidate the properties and benefits of liquid L-PRF products.

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