scholarly journals Biomimetic Implant Surface Functionalization with Liquid L-PRF Products: In Vitro Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Marco Lollobrigida ◽  
Manuela Maritato ◽  
Giuseppina Bozzuto ◽  
Giuseppe Formisano ◽  
Agnese Molinari ◽  
...  
Keyword(s):  
Autologous Blood ◽  
Combined Treatment ◽  
Pure Titanium ◽  
In Vitro Study ◽  
Vitro Study ◽  
Adjunctive Treatment ◽  
Machined Surface ◽  
Implant Surface ◽  
Platelet Rich Fibrin

Objective. Platelet-rich fibrin (PRF) clots and membranes are autologous blood concentrates widely used in oral surgical procedures; less is known, however, about the liquid formulations of such products. The aim of this in vitro study is to assess the behavior of different implant surfaces when in contact with two liquid leucocyte- and platelet-rich fibrin (L-PRF) products.Methods. Six commercial pure titanium discs, of 9.5 mm diameter and 1.5 mm thickness, were used. Three of these samples had a micro/nano-rough surface; three were machined. Three different protocols were tested. Protocols involved the immersion of the samples in(1)a platelets, lymphocytes, and fibrinogen liquid concentrate (PLyF) for 10 minutes,(2)an exudate obtained from L-PRF clots rich in fibronectin and vitronectin for 5 minutes, and(3)the fibronectin/vitronectin exudate for 2 minutes followed by immersion in the PLyF concentrate for further 8 minutes. After these treatments, the samples were fixed and observed using a scanning electron microscope (SEM).Results. Under microscopic observation,(1)the samples treated with the PLyF concentrate revealed a dense fibrin network in direct contact with the implant surface and a significant number of formed elements of blood;(2)in the samples treated with the fibronectin/vitronectin exudates, only a small number of white and red blood cells were detectable; and(3)in samples exposed to the combined treatment, there was an apparent increase in the thickness of the fibrin layer. When compared to the machined surface, the micro/nano-rough samples showed an overall increased retention of fibrin, leading to a thicker coating.Conclusions. Liquid L-PRF products promote the formation of a dense fibrin clot on micro/nano-rough implant surfaces in vitro. The adjunctive treatment of surfaces with the fibronectin/vitronectin exudate could provide support to contact of the fibrin with the surface, though it is not essential for the clot formation. Further studies are necessary to better elucidate the properties and benefits of liquid L-PRF products.

scholarly journals The effect of platelet-rich fibrin on normal dermal fibroblast proliferation after mitomycin-c treatment: An in vitro study

2021 ◽  
Vol 62 ◽  
pp. 473-476
Author(s):  
Ishandono Dachlan ◽  
Hendy Satrya Kurniawan ◽  
Aditya Wicaksana ◽  
Aditya Rifqi Fauzi ◽  
Firdian Makrufardi ◽  
...  
Keyword(s):  
Mitomycin C ◽  
Dermal Fibroblast ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fibroblast Proliferation ◽  
Platelet Rich Fibrin

scholarly journals Evaluation of histological and pH changes in platelet-rich fibrin and platelet-rich fibrin matrix: A In vitro study

2019 ◽  
Vol 10 (4) ◽  
pp. 652
Author(s):  
RajanikanthB Rajaram ◽  
Shruthi Nagaraja ◽  
Sylvia Mathew ◽  
C Pushpalatha ◽  
Anil Abraham ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Platelet Rich Fibrin ◽  
Ph Changes ◽  
Fibrin Matrix

Bioactivity and Surface Characteristics of Titanium Implants Following Various Surface Treatments: An In Vitro Study

2015 ◽  
Vol 41 (5) ◽  
pp. e183-e188 ◽  
Author(s):  
Aswini Kumar K ◽  
Vinaya Bhatt ◽  
Manilal Balakrishnan ◽  
Mohamed Hashem ◽  
Sajith Vellappally ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Calcium Ions ◽  
In Vitro Study ◽  
Surface Treatments ◽  
Titanium Implants ◽  
Vitro Study ◽  
Acid Etching ◽  
Implant Surface ◽  
Group A

This study compared the surface topography, hydrophilicity, and bioactivity of titanium implants after 3 different surface treatments (sandblasting and acid etching, modified sandblasting and acid etching, and thermal oxidation) with those of machined implants. One hundred indigenously manufactured threaded titanium implants were subjected to 3 methods of surface treatment. The surface roughness of the nontreated (Group A) and treated samples (Groups B through D) was evaluated with a scanning electron microscope (SEM) and profilometer. The wettability was visually examined using a colored dye solution. The calcium ions attached to the implant surface after immersing in simulated body fluid (SBF) were assessed on days 1, 2, and 7 with an atomic electron spectroscope. The data were analyzed statistically. The SBF test allowed the precipitation of a calcium phosphate layer on all surface-treated samples, as evidenced in the SEM analysis. A significantly higher amount of calcium ions and increased wettability were achieved in the thermally oxidized samples. The mean roughness was significantly lower in Group A (0.85 ± 0.07) compared to Group B (1.35 ± 0.17), Group C (1.40 ± 0.14), and Group D (1.36 ± 0.18). The observations from this in vitro study indicated that surface treatment of titanium improved the bioactivity. Moreover, results identified the implants that were sandblasted, acid etched, and then oxidized attracted more calcium ions.

scholarly journals Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

2020 ◽  
Vol 35 (1) ◽  
pp. 83-96 ◽  
Author(s):  
Solomiya Kyyak ◽  
Sebastian Blatt ◽  
Andreas Pabst ◽  
Daniel Thiem ◽  
Bilal Al-Nawas ◽  
...  
Keyword(s):  
Bone Substitute ◽  
In Vitro Study ◽  
Vitro Study ◽  
Bone Morphogenetic Protein 2 ◽  
Bone Substitute Material ◽  
Platelet Rich Fibrin ◽  
Osteoblast Activity ◽  
Substitute Material ◽  
Xenogenic Bone

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

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