scholarly journals A comprehensive review of surgical margin in oral squamous cell carcinoma highlighting the significance of tumor-free surgical margins

2019 ◽  
Vol 15 (3) ◽  
pp. 449 ◽  
Author(s):  
Mamata Kamat ◽  
BhagawanDas Rai ◽  
RudrayyaS Puranik ◽  
UmaVasant Datar
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Surgical Margin ◽  
Surgical Margins ◽  
Comprehensive Review

scholarly journals Comparative Study of Acrylic Color and India Ink for Their Use as a Surgical Margin Inks in Oral Squamous Cell Carcinoma

2015 ◽  
Vol 6 (1) ◽  
pp. 26-30 ◽  
Author(s):  
Anuprita Patil ◽  
Rahul Anand ◽  
Pratiksha Mahajan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Comparative Study ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Surgical Margin ◽  
Surgical Margins ◽  
Drying Time ◽  
India Ink ◽  
Hematoxylin And Eosin Stain

ABSTRACT Objective To compare acrylic color and India ink for their use as a surgical margin. Since, acrylic paints are available in multiple colors, they can be used to mark different surgical margins with different specific colors. Materials and methods The study was carried out on 30 surgically excised and formalin fixed oral squamous cell carcinoma (OSCC) specimens. Four surgical margins of each 15 specimens were marked with India ink and the remaining were marked with acrylic colors. The grossed specimens were then processed using routine histopathology technique. The 4 mm sections of tissue block were cut using microtome and sections were stained with hematoxylin and eosin stain. Various macroscopic and microscopic parameters were used to study the suitability of acrylic color as surgical ink. Results Three different technicians found equal ease of application for acrylic color and India ink. The drying time for acrylic color (1.30 ± 0.009 minutes) was significantly less than India ink (2.30 ± 0.05 minutes). Out of total 60 blocks made for acrylic colors, 52 (86.66%) displayed clear visibility on paraffin blocks (93.33%). In contrast, out of 60 blocks made for India ink, only 34 showed faint visibility on paraffin blocks (56.66%). All acrylic colors were visible very clearly and uninterruptedly under the microscope, while three (5%) surgical margins stained with India ink were interrupted. With the acrylic colors, no contamination or staining of other tissues, processing fluids and solutions were reported. However, with India ink, three (5%) samples reported contamination in alcohol and xylene. There was no interference with the microscopic interpretation of nuclear and cellular details for both acrylic color and India ink whatsoever. Conclusion Acrylic colors are more suitable as surgical ink over India ink because of its availability in different colors, ease of application, faster drying time, and no contamination with processing fluids, easy availability and excellent visibility on paraffin blocks and under microscope. Moreover, if needed, painting with multiple colors could also help in reassessment of surgical margins of the gross specimen in the future. How to cite this article Sarode SC, Sarode GS, Patil S, Mahajan P, Anand R, Patil A. Comparative Study of Acrylic Color and India Ink for Their Use as a Surgical Margin Inks in Oral Squamous Cell Carcinoma. World J Dent 2015;6(1):26-30.

scholarly journals DNA Methylation Markers from Negative Surgical Margins Can Predict Recurrence of Oral Squamous Cell Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2915
Author(s):  
Bruna Pereira Sorroche ◽  
Fazlur Rahman Talukdar ◽  
Sheila Coelho Soares Lima ◽  
Matias Eliseo Melendez ◽  
Ana Carolina de Carvalho ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tumor Recurrence ◽  
Surgical Margins ◽  
Risk Scores ◽  
Marker Panel ◽  
Specificity And Sensitivity

The identification of molecular markers in negative surgical margins of oral squamous cell carcinoma (OSCC) might help in identifying residual molecular aberrations, and potentially improve the prediction of prognosis. We performed an Infinium MethylationEPIC BeadChip array on 32 negative surgical margins stratified based on the status of tumor recurrence in order to identify recurrence-specific aberrant DNA methylation (DNAme) markers. We identified 2512 recurrence-associated Differentially Methylated Positions (DMPs) and 392 Differentially Methylated Regions (DMRs) which were enriched in cell signaling and cancer-related pathways. A set of 14-CpG markers was able to discriminate recurrent and non-recurrent cases with high specificity and sensitivity rates (AUC 0.98, p = 3 × 10−6; CI: 0.95–1). A risk score based on the 14-CpG marker panel was applied, with cases classified within higher risk scores exhibiting poorer survival. The results were replicated using tumor-adjacent normal HNSCC samples from The Cancer Genome Atlas (TCGA). We identified residual DNAme aberrations in the negative surgical margins of OSCC patients, which could be informative for patient management by improving therapeutic intervention. This study proposes a novel DNAme-based 14-CpG marker panel as a promising predictor for tumor recurrence, which might contribute to improved decision-making for the personalized treatment of OSCC cases.

Impact of dysplastic surgical margins for patients with oral squamous cell carcinoma

Oral Oncology ◽  
2019 ◽  
Vol 97 ◽  
pp. 1-6 ◽  
Author(s):  
Tseng-Cheng Chen ◽  
Huei-Lun Chang ◽  
Tsung-Lin Yang ◽  
Pei-Jen Lou ◽  
Yih-Leong Chang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Surgical Margins

Significance of post-resection tissue shrinkage on surgical margins of oral squamous cell carcinoma

2015 ◽  
Vol 43 (4) ◽  
pp. 475-482 ◽  
Author(s):  
Hossam Abdelkader El-Fol ◽  
Samer Abduljabar Noman ◽  
Mohamed Galal Beheiri ◽  
Abdalla M. Khalil ◽  
Mahmoud Mohamed Kamel
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Surgical Margins ◽  
Tissue Shrinkage

Intraoperative Imprint Evaluation of Surgical Margins in Oral Squamous Cell Carcinoma

2013 ◽  
Vol 57 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Gatha Singh Yadav ◽  
Mandana Donoghue ◽  
David P. Tauro ◽  
Ashutosh Yadav ◽  
Sumit Agarwal
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Surgical Margins

Influence of surgical margins on local recurrence in T1/T2 oral squamous cell carcinoma

Head & Neck ◽  
2014 ◽  
Vol 37 (8) ◽  
pp. 1176-1180 ◽  
Author(s):  
Conor P. Barry ◽  
Ferhan Ahmed ◽  
Simon N. Rogers ◽  
Derek Lowe ◽  
Fazilet Bekiroglu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Local Recurrence ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Surgical Margins

scholarly journals Intraoperative Assessment of Surgical Margins of Oral Squamous Cell Carcinoma Using Frozen Sections: A Practical Clinicopathological Management for Recurrences

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Shun Miyota ◽  
Takanori Kobayashi ◽  
Tatsuya Abé ◽  
Hisashi Miyajima ◽  
Masaki Nagata ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Free Group ◽  
Squamous Cell ◽  
Frozen Section ◽  
Surgical Margins ◽  
Clinical Issue ◽  
Intraoperative Assessment ◽  
Relapse Group

Background. Local recurrence remains a challenging clinical issue for the treatment of oral squamous cell carcinoma (SCC). We analyzed retrospectively how effective the frozen section technique (FS) was against recurrences of oral SCC.Methods. We screened 343 surgical samples from 236 patients who had oral SCC, carcinoma in situ (CIS), or epithelial dysplasia, and we followed up their clinical outcomes for at least 5 years. Histopathological states of surgical margins were compared between FS and surgical materials in relapse and relapse-free groups, respectively.Results. Among the 236 patients, 191 were classified into the relapse-free group, and 45 into the relapse group. FS was more frequently performed in the relapse-free group (128/191) than in the relapse group (83/152). Histopathologically, moderate dysplasia or CIS (borderline malignancies) and SCC were recognized in 55 samples of the relapse-free group and in 57 of the relapse group. For those surgical margins with borderline malignancies, additional incisions were performed in 38 of the 55 relapse-free cases, which reduced to 20 from the 38 margins with borderline malignancies (47.4% reduction), and in 39 of the 57 relapse cases, which reduced to only 3 of 39 (7.7% reduction).Conclusions. The intraoperative assessment of surgical margins by FS is essential in preventing recurrences of oral mucosal malignancies.

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