scholarly journals Punica granatum as a salutiferous superfruit in the treatment of oral candidiasis – An in-vitro study

2020 ◽  
Vol 24 (1) ◽  
pp. 188
Author(s):  
SShiny Samlin ◽  
KS Prem Kumar ◽  
B Siva ◽  
R Sudharshan ◽  
A Vignesswary ◽  
...  
Keyword(s):  
Oral Candidiasis ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Vitro Study

Evaluation of the effect of a heat-killed lactic acid bacterium, Enterococcus faecalis 2001, on oral candidiasis

2019 ◽  
Vol 10 (6) ◽  
pp. 661-669
Author(s):  
T. Yamazaki ◽  
R. Ushikoshi-Nakayama ◽  
K. Shirone ◽  
M. Suzuki ◽  
S. Abe ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Clinical Study ◽  
Enterococcus Faecalis ◽  
Mycelial Growth ◽  
Oral Candidiasis ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Vitro Study ◽  
Oral Candida

The effect of a preparation of heat-killed Gram-positive lactic acid bacteria Enterococcus faecalis 2001 (EF-2001) on oral candidiasis was evaluated by two studies. An in vitro study was performed to assess the inhibitory effect on mycelial growth of Candida strains isolated from a patient with oral candidiasis, and a clinical study was done in patients with oral candidiasis. In the in vitro study, EF-2001 inhibited mycelial growth of IT-1, a Candida strain isolated from a patient with oral candidiasis, at concentrations ≥2.34×109 cells/ml. An open clinical study was performed in 13 patients with oral candidiasis. The subjects took a powder containing 7.5×1011 heat-killed EF-2001 once a day before bedtime for seven consecutive days. In 11 of the 12 patients available for analysis (92%), the oral Candida load (cfu/swab) showed a significant decrease (P=0.01079, d=-0.437). There was a 55% decrease of Candida albicans and a 93.8% decrease of Candida glabrata. The following symptoms showed significant improvement: tinnitus (P=0.048, d=-0.462), cold feeling (P=0.048, d=-0.463), and depression (P=0.019, d=-0.34). In addition, 4 out of 26 oral symptoms tended to improve. These results suggest that EF-2001 significantly decreased the oral Candida load in patients with oral candidiasis by inhibiting mycelial growth and that EF-2001 is an effective treatment for oral candidiasis.

scholarly journals HEPATOPROTECTIVE EFFECTS OF PUNICA GRANATUM FRUIT AGAINST D-GALACTOSAMINE-INDUCED HEPATOTOXICITY IN RATS: IN VITRO AND IN VIVO STUDIES

2017 ◽  
Vol 9 (8) ◽  
pp. 168
Author(s):  
Mohd Amir ◽  
Mohd Nasar Mallick ◽  
Niyaz Ahmad ◽  
Abuzer Ali ◽  
Sayeed Ahmad ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
In Vitro Study ◽  
Punica Granatum ◽  
Vitro Study ◽  
Glutamate Oxaloacetate Transaminase ◽  
Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate ◽  
Serum Glutamate

Objective: Hepatoprotective activity of Punica granatum Linn. (Family: Punicaceae) was evaluated by in vitro and in vivo model. HepG2 cell lines were used for in vitro study and D-Galactosamine (D-GalN) induced hepatic damage model for in vivo evaluation.Methods: Hepatoprotective potential was assessed by measuring serum level of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, alkaline phosphatase, total bilirubin, and albumin. Enzymatic antioxidant parameters like TBARs (thiobarbituric acid reactive substances), GSH (glutathione), SOD (superoxide dismutase) and CAT (catalase) also evaluated in this study. Results: Rats treated with D-GalN showed a significant increase in serum levels of SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase), ALP (alkaline phosphatase), bilirubin and TBARs, reflecting liver damage. The in vitro study indicates a beneficial effect of aqueous extract of P. granatum in comparison with methanolic extract, on D-GalN induced toxicity to HepG2 cells. On the basis of in vitro study, aqueous extract was selected for in vivo evaluation. The aqueous extract of P. granatum significantly reduced the elevated serum biomarkers, indicating the recovery of hepatocellular injury. It was also observed that D-GalN induced a significant decrease in GSH, SOD, CAT, protein, and albumin level were increased on the treatment of the rats with aqueous extract of P. granatum. The findings were also confirmed by histopathological studies. Periportal area and extensive hepatocyte damage and haemorrhage are seen in D-GalN treated group. The portal triad with less degree of inflammatory cell infiltration around the bile duct is seen in P. granatum (500 mg/kg)+(D-GalN) treated group.Conclusion: Results of this study revealed that P. granatum fruit could afford a significant protection for the alleviation of hepatic toxicity. Possible mechanism may involve its action against oxidative stress. 

scholarly journals Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study

2012 ◽  
Vol 2 (4) ◽  
pp. 129-135 ◽  
Author(s):  
Prabha Shakya ◽  
Deshraj Jain ◽  
Tripty Rahangdale
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
In Vitro Study ◽  
Acrylic Resin ◽  
Vitro Study ◽  
Degree Of Contamination ◽  
Colony Forming Units ◽  
The Mean ◽  
Level Of Significance

ABSTRACT Purpose Denture-related stomatitis is probably the most common form of oral candidiasis and its reported prevalence varies widely ranging up to 65%. In this in vitro study, we evaluate the effect of delmopinol application on Candida albicans adherence on heat-cured acrylic resin, cold-cured acrylic resin and microwave-cured acrylic resin. Materials and methods A total of 40 specimens of each type of acrylic resin were made; 20 specimens of each type were contaminated before delmopinol treatment and 20 specimens were contaminated after delmopinol treatment. The each specimen in each tube was individually transferred to a spectrophotometer at 530 nm wavelength in order to measure the turbidity degree, through the transmittance. Aliquots of 10 μl of each tube was then collected and inoculated into agar Sabouraud plates containing 500 mmol/l of sucrose, which was incubated for 24 hours at 37oC, in order to check microbial growth. Two-way ANOVA analysis of variance test and posthoc Turkey's test were carried out to ascertain the level of significance (p < 0.001) of various observations. Results Mean of the turbidity degree of contamination after delmopinol application for heat-cured acrylic resin specimens (A1) 1440.80, colony forming units (CFU) (⨯106/ml), cold-cured acrylic resin specimens (B1) 833.30 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C1) 944.70 CFU (⨯106/ml) was significantly higher than the mean of the turbidity degree of contamination before delmopinol application for heatcured acrylic resin specimens (A2) 550.85 CFU (⨯106/ml), coldcured acrylic resin specimens (B2) 341.45 CFU (⨯106/ml) and microwave-cured acrylic resin specimens (C2) 451.50 CFU (⨯106/ml). Conclusion In case of contamination after delmopinol application, heat-cured acrylic resin showed maximum reduction in adherence of C. albicans in the study. How to cite this article Shakya P, Jain D, Rahangdale T. Evaluation and Comparison of Effect of Delmopinol Application on Adherence of Candida albicans on Denture Fitting Surface on Three Types of Acrylic Resin: An in vitro Study. Int J Prosthodont Restor Dent 2012;2(4):129-135.

Interleukin-10 as an indicator of chronic course of oral candidiasis in diabetics: an in vitro study

2015 ◽  
Vol 36 (1) ◽  
pp. 120-124 ◽  
Author(s):  
Mayuri Rani ◽  
Shukla Das ◽  
Vishnampettai Ganapathysubramanian Ramachandran ◽  
Sajad Ahmad Dar ◽  
Gajender Singh Ranga ◽  
...  
Keyword(s):  
Oral Candidiasis ◽  
In Vitro Study ◽  
Interleukin 10 ◽  
Vitro Study ◽  
Chronic Course
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