Effect of post-ruminal infusion of glutamine on plasma amino acids, milk yield and composition in lactating dairy cows

2001 ◽  
Vol 81 (2) ◽  
pp. 229-235 ◽  
Author(s):  
J. C. Plaizier ◽  
J. -P. Walton ◽  
B. W. McBride
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Composition ◽  
Plasma Amino Acid ◽  
Dry Matter Intake ◽  
Plasma Amino Acids

The objectives of this study were to examine the effect of supplying post-ruminal L-glutamine in mid-lactation Holstein dairy cows on plasma amino acid profile, dry matter intake, milk yield and milk composition. The experiment was designed as a 4 × 4 Latin square with four 2-wk periods. Cows were continuously infused post-ruminally with graded levels of L-glutamine (L-Gln) for 5 consecutive days during the second week of each period. During the last 24 h of the infusion, blood plasma was collected every 6 h for the determination of plasma amino acid concentrations. Plasma Gln concentration increased (P < 0.05) with increasing L-Gln daily infusions. Infusion of 0, 100, 200, and 300 g d–1 resulted in blood plasma Gln concentrations of 289.5, 299.2, 356.4 and 386.2 mmol L–1, respectively. The level of Gln infusion also resulted in a decrease in the blood plasma concentration of phenylalanine, but the concentrations of all other amino acids were not affected. The administration of Gln did not affect dry matter intake, milk yield and milk composition. Key words: Post-ruminal, L-glutamine, plasma amino acids, milk constituents

scholarly journals Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

2008 ◽  
Vol 17 (4) ◽  
pp. 351 ◽  
Author(s):  
L. KRÍZOVÁ ◽  
J. TRINÁCTÝ ◽  
M. RICHTER
Keyword(s):  
Amino Acids ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Protein ◽  
Experimental Period ◽  
Dry Matter Intake ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Milk Protein Content ◽  
Ad Libitum

The aim of this study was to determine the influence of leucine supplement in the form of rumen-protected tablets on milk yield and composition and plasma amino acids in four high-yielding lactating Holstein cows. The experiment was carried out as a cross-over procedure and was divided into 4 periods of 14 d (10 d preliminary period and 4 d experimental period). Cows were fed ad libitum a diet based on maize silage, lucerne hay and a supplemental mixture. The diet, defficient in methionine, lysine, and leucine, was supplemented with methionine+lysine (Control) or methionine+lysine+leucine (Leu) in rumen protected form. The dry matter intake, milk yield and milk yield expressed in energy corrected milk did not differ significantly between the treatments. Milk protein content and yield did not show statistically significant variation. The contents and yield of casein, fat, lactose and urea were unaffected by the treatment. Blood metabolites did not vary between the treatments. The introduction of Leu resulted in higher plasma levels of proline (p

Response of lactating dairy cows to dietary protein from canola meal or distillers’ grains on dry matter intake, milk production, milk composition, and amino acid status

2015 ◽  
Vol 95 (2) ◽  
pp. 267-279 ◽  
Author(s):  
I. P. Acharya ◽  
D. J. Schingoethe ◽  
K. F. Kalscheur ◽  
D. P. Casper
Keyword(s):  
Amino Acid ◽  
Dairy Cows ◽  
Milk Production ◽  
Dietary Protein ◽  
Dry Matter ◽  
Milk Composition ◽  
Distillers Grains ◽  
Dry Matter Intake ◽  
Canola Meal ◽  
Acid Status

Acharya I. P., Schingoethe D. J., Kalscheur K. F. and Casper D. P. 2015. Response of lactating dairy cows to dietary protein from canola meal or distillers’ grains on dry matter intake, milk production, milk composition, and amino acid status. Can. J. Anim. Sci. 95: 267–279. A study was conducted to determine the response of feeding two different crude protein (CP) concentrations [low protein (LP, 14.3% CP) and high protein (HP, 16.3% CP)] and sources of protein [canola meal (CM) and high-protein dried distillers’ grains (HPDDG)] on dry matter intake, milk production and composition, and amino acid (AA) status of high producing dairy cows. Sixteen Holstein cows were used in a replicated 4×4 Latin square having a 2×2 factorial arrangement of treatments. All diets contained 55% forage (50% alfalfa hay and 50% corn silage) and 45% concentrate. Diets were formulated to contain LP with CM, LP with HPDDG, HP with CM and HP with HPDDG. Experimental feeding periods were 4 wk with data collected during weeks 3 and 4 of each period. Dry matter intake (24.6 vs. 25.9 kg d−1 for LP and HP, respectively) was increased for cows fed the higher CP diets, milk yield was greater for cows fed HP diets (34.0 vs. 36.4 kg d−1), and milk fat percentage was greater for cows fed HP compared with LP, but were similar between dietary protein sources. Feed efficiency calculated as fat-corrected milk and energy-corrected milk was greater for cows fed HP than LP, but was similar between sources. Dietary metabolizable protein of CM diets was utilized more efficiently than from HPDDG diets for milk protein production. Mammary gland extraction efficiency of essential AA indicated that Met was the first limiting AA for CM-based diets while Lys was first limiting for HPDDG-based diets. In summary, canola meal and distillers’ grains are both good quality protein supplements for lactating cows.

scholarly journals Effect of duodenal infusions of leucine on milk yield and plasma amino acids in dairy cows

2010 ◽  
Vol 55 (No. 9) ◽  
pp. 351-358 ◽  
Author(s):  
M. Richter ◽  
J. Svobodová ◽  
L. Křížová ◽  
J. Třináctý ◽  
P. Homolka
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Basal Diet ◽  
Experimental Period ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Subsequent Period ◽  
Period 2

Four high-yielding lactating Holstein cows fitted with duodenal cannulas were used in the experiment. Cows were divided into 2 groups &ndash; control (Control) with leucine deficiency and experimental (Leucine) with a leucine supplement. The experiment was divided into 4 periods of 7 days, each consisting of a 3-day preliminary period followed by a 4-day experimental period. In the first period, 2 cows were assigned to Control and the remaining 2 to Leucine. In the subsequent period the cows were switched to the other treatment. Cows were fed individually twice daily the basal diet based on maize silage, lucerne hay and supplemental mixture. Infusions of amino acids in Leucine consisted of methionine (12.6 g/day), lysine (20.7 g/day), histidine (10.7 g/day) and leucine (19.3 g/day). The composition of amino acid infusate in Control was the same except for leucine that was replaced with monosodium L-glutamate. The intake of dry matter was not affected by the treatment (P &gt; 0.05). No effect of leucine infusion on milk yield and composition was observed (P &gt; 0.05), nevertheless the concentration of protein and casein in milk tended to be higher in Leucine (38.3 and 31.3 g/kg) than in Control (37.4 and 30.4 g/kg, respectively, P &lt; 0.1). The yield of milk components was not affected by the treatment (P &gt; 0.05). Duodenal infusion of leucine resulted in a decreased plasma level of isoleucine in Leucine compared to Control (P &lt; 0.01). Concentrations of leucine, cysteine and citrulline tended to be higher and the concentration of tyrosine tended to be lower in Leucine in comparison with Control (P &lt; 0.10).

Effects of dried and ensiled tomato pomace on dry matter intake, milk yield and composition of dairy cows in Iran

2007 ◽  
Vol 2007 ◽  
pp. 191-191
Author(s):  
R Safari ◽  
R Valizadeh ◽  
F Efteljaro Shahroudi ◽  
A Tahmasebi ◽  
J. Bayat
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Composition ◽  
Dry Matter Intake ◽  
High Moisture ◽  
Tomato Processing ◽  
Tomato Pulp ◽  
Summer Time ◽  
Fresh Tomato

In Khorasan province of Iran about 1 million tonnes of tomatoes are grown annually. They are either directly sold to the customers as vegetable (200,000 tonnes) or further processed to products such as paste, ketchup, sauces etc. After the juice is extracted from fresh tomatoes, a residue primarily consisting of water, tomato seeds and peels is left. Tomato processing residues which is called tomato pulp accounts about one fifth of fresh weight or 160,000 tonnes in the area (ministry of Jahad-Agriculture, 2004). Fresh tomato pulp becomes sour and mould rapidly because it is traditionally processed during summer time and has high moisture content. Consequently, it is advisable to be ensiled or dried. Although it is commonly fed to ruminants but little data is available on its effect on milk yield, milk composition and dry matter intake in dairy cows.

scholarly journals The effect of duodenal infusion of histidine on milk yield, milk composition, and plasma amino acids in dairy cows

2012 ◽  
Vol 21 (4) ◽  
pp. 555-565 ◽  
Author(s):  
S. Hadrová ◽  
L. Křížová ◽  
M. Richter ◽  
J. Třináctý ◽  
M. Dračková
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Composition ◽  
Plasma Amino Acids

scholarly journals Predicting Daily Dry Matter Intake Using Feed Intake of First Two Hours after Feeding in Mid and Late Lactation Dairy Cows with Fed Ration Three Times Per Day

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 104
Author(s):  
Shulin Liang ◽  
Chaoqun Wu ◽  
Wenchao Peng ◽  
Jian-Xin Liu ◽  
Hui-Zeng Sun
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Prediction Equation ◽  
Dry Matter Intake ◽  
Validation Dataset ◽  
Nutrient Requirements ◽  
Independent Dataset ◽  
Lactating Dairy Cows ◽  
Proposed Model

The objective of this study was to evaluate the feasibility of using the dry matter intake of first 2 h after feeding (DMI-2h), body weight (BW), and milk yield to estimate daily DMI in mid and late lactating dairy cows with fed ration three times per day. Our dataset included 2840 individual observations from 76 cows enrolled in two studies, of which 2259 observations served as development dataset (DDS) from 54 cows and 581 observations acted as the validation dataset (VDS) from 22 cows. The descriptive statistics of these variables were 26.0 ± 2.77 kg/day (mean ± standard deviation) of DMI, 14.9 ± 3.68 kg/day of DMI-2h, 35.0 ± 5.48 kg/day of milk yield, and 636 ± 82.6 kg/day of BW in DDS and 23.2 ± 4.72 kg/day of DMI, 12.6 ± 4.08 kg/day of DMI-2h, 30.4 ± 5.85 kg/day of milk yield, and 597 ± 63.7 kg/day of BW in VDS, respectively. A multiple regression analysis was conducted using the REG procedure of SAS to develop the forecasting models for DMI. The proposed prediction equation was: DMI (kg/day) = 8.499 + 0.2725 × DMI-2h (kg/day) + 0.2132 × Milk yield (kg/day) + 0.0095 × BW (kg/day) (R2 = 0.46, mean bias = 0 kg/day, RMSPE = 1.26 kg/day). Moreover, when compared with the prediction equation for DMI in Nutrient Requirements of Dairy Cattle (2001) using the independent dataset (VDS), our proposed model shows higher R2 (0.22 vs. 0.07) and smaller mean bias (−0.10 vs. 1.52 kg/day) and RMSPE (1.77 vs. 2.34 kg/day). Overall, we constructed a feasible forecasting model with better precision and accuracy in predicting daily DMI of dairy cows in mid and late lactation when fed ration three times per day.

Source and function of urinary ammonia in the alligator

1959 ◽  
Vol 197 (4) ◽  
pp. 873-879 ◽  
Author(s):  
Roland A. Coulson ◽  
Thomas Hernandez
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Level ◽  
Amino Acid Level ◽  
Plasma Amino Acid ◽  
Plasma Amino Acids ◽  
Renal Reabsorption ◽  
And Function ◽  
Phosphate Solutions

The rate of renal deamination of 18 amino acids was determined by injecting them into alligators and measuring the ammonia excreted. Not only did glycine, alanine, glutamine and leucine account for nearly half of the plasma amino acids, they were also deaminated more rapidly than any of the others. In view of this it was concluded that these four amino acids are the natural precursors of urinary NH3 in the alligator. Increased NH3 and CO2 excretion following glycine injections resulted in increased renal reabsorption of Na and Cl when NaCl was injected and increased Na reabsorption when NaHCO3 or Na phosphate solutions were injected. The fact that excess NH4HCO3 excretion enhances salt reabsorption independent of plasma pH makes it probable that the excretion of N is the chief function of the ammonia mechanism and that salt conservation is incidental. Insulin decreased the plasma amino acid level and drastically reduced the NH3 excretion. With the decrease in ammonia, NaCl and NaHCO3 were excreted in increased amounts.

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