scholarly journals Effect of duodenal infusions of leucine on milk yield and plasma amino acids in dairy cows

2010 ◽  
Vol 55 (No. 9) ◽  
pp. 351-358 ◽  
Author(s):  
M. Richter ◽  
J. Svobodová ◽  
L. Křížová ◽  
J. Třináctý ◽  
P. Homolka
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Basal Diet ◽  
Experimental Period ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Subsequent Period ◽  
Period 2

Four high-yielding lactating Holstein cows fitted with duodenal cannulas were used in the experiment. Cows were divided into 2 groups – control (Control) with leucine deficiency and experimental (Leucine) with a leucine supplement. The experiment was divided into 4 periods of 7 days, each consisting of a 3-day preliminary period followed by a 4-day experimental period. In the first period, 2 cows were assigned to Control and the remaining 2 to Leucine. In the subsequent period the cows were switched to the other treatment. Cows were fed individually twice daily the basal diet based on maize silage, lucerne hay and supplemental mixture. Infusions of amino acids in Leucine consisted of methionine (12.6 g/day), lysine (20.7 g/day), histidine (10.7 g/day) and leucine (19.3 g/day). The composition of amino acid infusate in Control was the same except for leucine that was replaced with monosodium L-glutamate. The intake of dry matter was not affected by the treatment (P > 0.05). No effect of leucine infusion on milk yield and composition was observed (P > 0.05), nevertheless the concentration of protein and casein in milk tended to be higher in Leucine (38.3 and 31.3 g/kg) than in Control (37.4 and 30.4 g/kg, respectively, P < 0.1). The yield of milk components was not affected by the treatment (P > 0.05). Duodenal infusion of leucine resulted in a decreased plasma level of isoleucine in Leucine compared to Control (P < 0.01). Concentrations of leucine, cysteine and citrulline tended to be higher and the concentration of tyrosine tended to be lower in Leucine in comparison with Control (P < 0.10).

scholarly journals Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

2008 ◽  
Vol 17 (4) ◽  
pp. 351 ◽  
Author(s):  
L. KRÍZOVÁ ◽  
J. TRINÁCTÝ ◽  
M. RICHTER
Keyword(s):  
Amino Acids ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Protein ◽  
Experimental Period ◽  
Dry Matter Intake ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Milk Protein Content ◽  
Ad Libitum

The aim of this study was to determine the influence of leucine supplement in the form of rumen-protected tablets on milk yield and composition and plasma amino acids in four high-yielding lactating Holstein cows. The experiment was carried out as a cross-over procedure and was divided into 4 periods of 14 d (10 d preliminary period and 4 d experimental period). Cows were fed ad libitum a diet based on maize silage, lucerne hay and a supplemental mixture. The diet, defficient in methionine, lysine, and leucine, was supplemented with methionine+lysine (Control) or methionine+lysine+leucine (Leu) in rumen protected form. The dry matter intake, milk yield and milk yield expressed in energy corrected milk did not differ significantly between the treatments. Milk protein content and yield did not show statistically significant variation. The contents and yield of casein, fat, lactose and urea were unaffected by the treatment. Blood metabolites did not vary between the treatments. The introduction of Leu resulted in higher plasma levels of proline (p

Effect of post-ruminal infusion of glutamine on plasma amino acids, milk yield and composition in lactating dairy cows

2001 ◽  
Vol 81 (2) ◽  
pp. 229-235 ◽  
Author(s):  
J. C. Plaizier ◽  
J. -P. Walton ◽  
B. W. McBride
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Composition ◽  
Plasma Amino Acid ◽  
Dry Matter Intake ◽  
Plasma Amino Acids

The objectives of this study were to examine the effect of supplying post-ruminal L-glutamine in mid-lactation Holstein dairy cows on plasma amino acid profile, dry matter intake, milk yield and milk composition. The experiment was designed as a 4 × 4 Latin square with four 2-wk periods. Cows were continuously infused post-ruminally with graded levels of L-glutamine (L-Gln) for 5 consecutive days during the second week of each period. During the last 24 h of the infusion, blood plasma was collected every 6 h for the determination of plasma amino acid concentrations. Plasma Gln concentration increased (P < 0.05) with increasing L-Gln daily infusions. Infusion of 0, 100, 200, and 300 g d–1 resulted in blood plasma Gln concentrations of 289.5, 299.2, 356.4 and 386.2 mmol L–1, respectively. The level of Gln infusion also resulted in a decrease in the blood plasma concentration of phenylalanine, but the concentrations of all other amino acids were not affected. The administration of Gln did not affect dry matter intake, milk yield and milk composition. Key words: Post-ruminal, L-glutamine, plasma amino acids, milk constituents

scholarly journals Effect of rumen-protected methionine, lysine or both on milk production and plasma amino acids of high-yielding dairy cows

2009 ◽  
Vol 54 (No. 6) ◽  
pp. 239-248 ◽  
Author(s):  
J. Třináctý ◽  
L. Křížová ◽  
M. Richter ◽  
V. Černý ◽  
J. Říha
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Milk Production ◽  
Latin Square ◽  
Experimental Period ◽  
Blood Metabolites ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Beta Casein ◽  
Copolymer Coating

The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both amino acids added in the form of rumen-protected (RP) tablets with copolymer coating to a diet of dairy cows on yield and composition of milk and concentration of plasma amino acids (AA). The experiment was carried out on four high-yielding lactating Holstein cows with average milk production of 33.5 kg/day in the form of Latin square design. The four treatments were as follows: C – control without AA supplementation, L – control plus supplement of RP Lys, M – control plus supplement of RP Met and ML – control plus supplement of RP Met and Lys. The experiment was divided into 4 periods. Each period (14 days) consisted of 10-day preliminary period and 4-day experimental period. Cows were fed a diet based on maize silage, lucerne hay and supplemental mixture. Average milk yield recorded in ML was 34.18 kg and was higher than that recorded in L or M (32.46 kg and 32.13 kg, respectively <I>P</I> < 0.05) and tended to be higher than in C (33.33 kg, <I>P</I> > 0.05). The content of protein and casein was higher in L and ML in comparison with C (<I>P</I> < 0.05) and tended to be higher than in M (<I>P</I> > 0.05). Protein yield in ML (1 054 g/day) was higher than that found in C, L or M (990, 998 or 968 g/day, respectively, <I>P</I> < 0.05). The same response was found for casein yield (<I>P</I> < 0.05). Although the proportion of individual casein fractions was not affected by the treatment, the yield of &alpha;- and &beta;-casein differed (<I>P</I> < 0.05) while the yield of &kappa;-casein was not affected by the treatment. Concentrations of blood metabolites, except for betahydroxybutyrate (BHB), were not changed. Plasma Met concentrations were increased (<I>P</I> < 0.05) in the M and ML group. Similar, but insignificant increases (<I>P</I> > 0.05) were also observed in plasma Lys in the L and ML group.

Effect of feeding hay vs. silages of various types to dairy cows on feed intake, milk composition and coagulation properties

2020 ◽  
Vol 87 (3) ◽  
pp. 334-340
Author(s):  
Elisa Manzocchi ◽  
Werner Hengartner ◽  
Michael Kreuzer ◽  
Katrin Giller
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Fixed Effects ◽  
Experimental Period ◽  
Milk Composition ◽  
Processing Technology ◽  
Maize Silage ◽  
Rennet Coagulation

AbstractThis research paper addresses the hypotheses (1) that milk produced from hay-fed cows differs from that of silage-fed cows and (2) that silage type has an important impact, too. Four diets differing in forage type but with equal estimated milk production potential and a forage:concentrate ratio of 0.85 : 0.15 were compared regarding their effect on feed intake, milk yield and milk properties. The forages tested were hay, grass silage, conventional short-chopped and long-chopped maize silage subjected to a novel processing technology (Shredlage®). Twenty-four dairy cows were fed two of the four diets in two consecutive runs in an incomplete (4 × 2) Latin-square design (n = 12 per diet). Each experimental period lasted 22 d, with 12 d of adaptation and 10 d of sampling. During sampling, feed intake and milk yield were recorded daily, milk composition and coagulation properties were determined four times. The composition of the diet ingredients was analysed weekly. Data were analysed with a mixed model considering feed, period and their interaction as fixed effects. Stage of lactation, milk yield and milk composition from the pre-experimental period were used as covariates in the model. Dry matter intake was lower with the long-chopped processed maize silage compared to the other three groups. There were some diet differences in intakes of net energy for lactation and absorbable protein in the duodenum, but this did not result in changes in milk yield. The milk fat content was higher with the grassland-based diets compared to the maize silage diets. No treatment effect on milk acidity and rennet coagulation properties was observed. In conclusion, there were no indications for specific physico-chemical properties of milk from a hay-based diet, and maize processing technology was not of large effect either. Future investigations should focus on sensory differentiation of the milk produced with different forages.

scholarly journals Effect of replacing maize grain by hybrid rye grain in the TMR on performance of mid-lactating dairy cows

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Patrycja Rajtar ◽  
Marek Sady ◽  
Paweł Górka ◽  
Sylvia Kehoe ◽  
Piotr Micek
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Dry Matter ◽  
Maize Silage ◽  
Rennet Coagulation ◽  
Cereal Grain ◽  
Hybrid Rye ◽  
Maize Grain

Abstract Grain from traditional varieties of rye is not commonly used in dairy cattle nutrition. However, new hybrid varieties of rye currently available are characterized by some nutritional and agrotechnical benefits. This paper deals with the hypothesis that rye grain derived from a hybrid variety may be an alternative for maize grain in diets for dairy cattle. Sixteen lactating Polish Holstein-Friesian cows were divided into two groups according to their parity (8 primi- and 8 multiparous), stage of lactation (106 ± 30 days after calving) and milk yield (34 ± 4 kg/day). Cows were fed a total mixed ration (TMR) containing grass silage and whole crop maize silage and 29.2% of the concentrate (in dry matter). The latter contained approximately 48% of either maize grain (M) or hybrid rye grain (HR) as a main source of cereal grain. Experimental diets were fed for 9-weeks. The use of HR as a substitute for M did not affect (P>0.05) dry matter intake and milk yield. There were no differences between treatment groups in the content of milk solids, amino acids, and fatty acid profile. However, substituting M by HR positively influenced composition of milk protein fractions by increasing the proportion of α-casein (37.0 vs 39.7%; P<0.01) and к-casein (6.5 vs 7.3%; P=0.02) as well as decreasing the proportion of β-casein (28.8 vs 27.8%; P=0.02) and sensory characteristics of the milk (body and texture and taste; P<0.05). In turn, the composition of the diet did not affect the technological suitability for processing of milk fat (acid and peroxide number, melting and solidification temperature), rennet coagulation time, heat stability or titratable acidity. This study has shown that hybrid rye grain may be an alternative for maize grain in a TMR based on grass and whole maize silage for mid-lactation dairy cows. Further studies are needed with higher proportion of hybrid rye grain in TMR or with other roughages used in a basal diet to fully determine efficiency of hybrid rye grain use in diets for dairy cows.

scholarly journals Effect of Feeding Improved Grass Hays and Eragrostis tef Straw Silage on Milk Yield, Nitrogen Utilization, and Methane Emission of Lactating Fogera Dairy Cows in Ethiopia

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1021
Author(s):  
Shigdaf Mekuriaw ◽  
Atsushi Tsunekawa ◽  
Toshiyoshi Ichinohe ◽  
Firew Tegegne ◽  
Nigussie Haregeweyn ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Methane Emission ◽  
Dry Matter ◽  
Basal Diet ◽  
Nitrogen Utilization ◽  
Dry Matter Intake ◽  
Eragrostis Tef ◽  
Tropical Regions ◽  
Natural Pasture

The nutritionally imbalanced poor-quality diet feeding is the major constraint of dairy production in tropical regions. Hence, alternative high-quality roughage-based diets are required to improve milk yield and reduce methane emission (CH4). Thus, we tested the effects of feeding natural pasture hay, improved forage grass hays (Napier and Brachiaria Hybrid), and treated crop residues (Eragrostis tef straw) on nutrient digestibility, milk yield, nitrogen balance, and methane emission. The eight lactating Fogera cows selected for the experiment were assigned randomly to a 4 × 4 Latin square design. Cows were housed in well-ventilated individual pens and fed a total mixed ration (TMR) comprising 70% roughage and 30% concentrate. The four roughage-based basal dietary treatments supplemented with formulated concentrate were: Control (natural pasture hay (NPH)); treated teff straw silage (TTS); Napier grass hay (NGH); and Brachiaria hybrid grass hay (BhH). Compared with the control diet, the daily milk yield increased (p < 0.01) by 31.9%, 52.9%, and 71.6% with TTS, NGH, and BhH diets, respectively. Cows fed BhH had the highest dry matter intake (8.84 kg/d), followed by NGH (8.10 kg/d) and TTS (7.71 kg/d); all of these intakes were greater (p = 0.01) than that of NPH (6.21 kg/d). Nitrogen digestibility increased (p < 0.01) from the NPH diet to TTS (by 27.7%), NGH (21.7%), and BhH (39.5%). The concentration of ruminal ammonia nitrogen was higher for cows fed NGH than other diets (p = 0.01) and positively correlated with plasma urea nitrogen concentration (R² = 0.45). Feeding TTS, NGH, and BhH hay as a basal diet changed the nitrogen excretion pathway from urine to feces, which can help protect against environmental pollution. Estimated methane yields per dry matter intake and milk yield were decreased in dairy cows fed BhH, NGH, and TTS diets when compared to cows fed an NPH diet (p < 0.05). In conclusion, feeding of TTS, NGH, and BhH roughages as a basal diet to lactating dairy cows in tropical regions improved nutrient intake and digestibility, milk yield, nitrogen utilization efficiency, and reduced enteric methane emission.

Responses of dairy cows to short-term heat stress in controlled-climate chambers

2017 ◽  
Vol 57 (7) ◽  
pp. 1233 ◽  
Author(s):  
J. B. Garner ◽  
M. Douglas ◽  
S. R. O. Williams ◽  
W. J. Wales ◽  
L. C. Marett ◽  
...  
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Experimental Period ◽  
Dry Matter Intake ◽  
Temperate Climate ◽  
Short Term ◽  
Temperature And Humidity ◽  
Humidity Index

The objective of the present research was to describe the physiological and production responses of lactating dairy cows during and after sudden exposure to temperate-climate heat-wave conditions, compared with cows in thermoneutral conditions. Twelve lactating multiparous Holstein–Friesian dairy cows were housed in controlled-climate chambers for 4 days. Six were exposed to a short-term temperature and humidity challenge (THc, diurnal temperature and humidity fluctuations inducing moderate heat stress; temperature humidity index 74–84) and six cows were exposed to thermoneutral conditions (THn, temperatur humidity index 55–61). Cows were also measured during a 7-day pre-experimental and 14-day post-experimental period. Physiological indicators of heat stress were measured, including rectal and vaginal temperature and respiration rate, which indicated that the THc in controlled-climate chambers induced moderate heat stress. The cows exposed to the 4-day THc reduced their milk yield by 53% and their dry-matter intake by 48%, compared with the cows in the THn treatment. Milk yield of THc cows returned to pre-experimental milk yield by Day 7 and dry-matter intake by Day 4 of the post-experimental period. The short-term heat challenge induced metabolic adaptations by mobilising adipose tissue, as indicated by increased non-esterified fatty acids, and amino acids from skeletal muscle, as indicated by increased urea nitrogen to compensate for reduced nutrient intake and increased energy expenditure. Endocrine responses included greater prolactin concentrations, which is associated with thermoregulation and water metabolism. The cows exposed to THc displayed production and physical responses that facilitated lower metabolic heat production and greater heat dissipation in an attempt to maintain homeostasis during the short-term heat exposure. These results indicated that the conditions imposed on the cows in the controlled-climate chambers were sufficient to induce heat-stress responses and adversely affected production in the lactating dairy cow, and the delay between the return to normal feed intake and milk yield following the heat challenge suggests a period of metabolic recovery was occurring.

scholarly journals Supplementing pasture-fed dairy cows with pasture silage, maize silage, Lotus silage or sulla silage in summer - does it increase production?

2002 ◽  
pp. 85-89
Author(s):  
S.L. Woodward ◽  
A.V. Chaves ◽  
G.C. Waghorn ◽  
P.G. Laboyrie
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Nutritive Value ◽  
Milk Composition ◽  
The Other ◽  
Control Treatment ◽  
Maize Silage ◽  
Silage Maize

Forages suitable for supplementing pasture-fed dairy cows over summer-autumn must provide adequate dry matter (DM) and increase milk yield above that produced by cows grazing normal ryegrass/white clover pasture, without compromising milk composition. A trial was conducted in January-February 2001 to compare the benefits obtained from feeding four types of silage. There were two silages that contained condensed tannins (CT) (birdsfoot trefoil (Lotus corniculatus) and sulla (Hedysarum coronarium)), maize silage or traditional ryegrass-dominant pasture silage, all fed at 5 kg DM/cow/d with restricted pasture. Cows on the restricted pasture (control) treatment and those fed the silage treatments were offered an allowance of 25 kg DM pasture/cow/d, while the full pasture cows were offered 50 kg DM pasture/cow/d. Silage supplementation, regardless of silage type, increased both total dry matter intake (DMI) and milk production compared with the restricted pasture treatment. Cows on the Lotus silage supplement, and the full pasture treatment had significantly higher milk yields than the other silage-supplemented cows, all of which had similar milk yields. For the Lotus silage treatment, the high milk yield was probably due to a combination of the higher nutritive value of the silage, and to the action of CT, because the total DMI of the cows on the Lotus silage was the same as that of cows on the pasture silage and maize silage. The high milk yield of the full pasture treatment was mainly a result of the cows having a significantly higher total DMI than cows on all the other treatments. This trial has demonstrated the potential benefit of silage supplementation, particularly with Lotus silage, for increased milksolids yield in summerautumn when low pasture growth rates and quality may otherwise limit production. Keywords: condensed tannins, dairy cows, Lotus, maize, milksolids, ryegrass, silage, sulla, supplementation

An evaluation of the effect of concentrate proportion of the diet during previous and present lactations on animal performance of two breeds of lactating dairy cows

2003 ◽  
Vol 2003 ◽  
pp. 24-24
Author(s):  
T.W.J. Keady ◽  
C.S. Mayne
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Experimental Period ◽  
Yield Response ◽  
Direct Effects ◽  
Animal Performance ◽  
Stage Of Lactation ◽  
Lactating Dairy Cows

Studies undertaken to evaluate long responses to concentrate feeding, normally assess the direct effects (i.e. the effects during the period of increased feeding). However in addition to the direct responses to concentrate feeding during the experimental period, it has been recognised that additional nutrients fed during one stage of lactation may result in improvements in animal performance in later lactation or in the subsequent lactation. Keadyet al. (2002) reported that the milk yield response to concentrate proportion in the diet differed between Holstein (HF) and Norwegian (NC) dairy cattle with responses of 1.4 and 0.8 kg corrected milk/kg concentrate dry matter (DM) respectively. The objective of the present study was to evaluate the effects of concentrate proportion in the diet in the previous lactation on animal performance in the subsequent lactation. The effect of concentrate proportion in the diet on animal performance of HF and NC dairy cattle was also evaluated.

The combination of the n-alkanes and 13C techniques to estimate individual dry matter intakes of herbage and maize silage by grazing dairy cows

2000 ◽  
Vol 135 (1) ◽  
pp. 47-55 ◽  
Author(s):  
S. C. GARCÍA ◽  
C. W. HOLMES ◽  
J. HODGSON ◽  
A. MACDONALD
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Maize Silage ◽  
Lactation Length ◽  
Randomized Design ◽  
Holstein Friesian ◽  
Faecal Samples ◽  
Daily Intakes ◽  
Grazing Dairy Cows

Two studies were conducted at Massey University in 1998. Expt 1 investigated the combined use of n-alkanes and 13C techniques for the estimation of individual proportions and total intakes of herbage (H) and maize silage (MS) by dairy cows. Expt 2 measured the variation in the amount of MS consumed by individual cows fed as a group. In Expt 1, six dry Holstein–Friesian cows were kept indoors and fed a diet containing one of the following H[ratio ]MS ratios (dry matter basis)[ratio ]100[ratio ]0, 80[ratio ]20, 60[ratio ]40, 40[ratio ]60, 20[ratio ]80 or 10[ratio ]90 for 25 days. Cows were dosed with a slow-release capsule containing 8 g of dotriacontane (C32) and 8 g of hexatriacontane (C36). Intake estimates were based on individual faecal samples collected twice daily during two 5-day periods. In Expt 2 (grazing trial), 12 early-lactation cows were selected from a commercial herd of 48 autumn-calving cows and blocked into pairs according to milk yield, lactation length and lactation number in a complete block randomized design. Within each pair, cows were randomly assigned to two treatments: supplemented, S (4 kg MS DM per cow after the morning milking in feed troughs) or not supplemented, NS. Another eight cows were randomly selected from the rest of the herd to increase the number of individual estimations of H and MS intakes. Cows grazed perennial ryegrass-white clover pasture during the rest of the day. In Expt 1, H[ratio ]MS ratios were not accurately predicted by the odd-chained n-alkanes, but there was a strong linear relationship between the concentration of 13C in faeces and actual H: MS ratios in the diet. The 13C method was therefore combined with the n-alkanes, resulting in accurate estimations of H and MS intakes. In Expt 2, a large variation was observed among individual cows in their daily intakes of MS (range 0·94 to 5·09 kg DM per cow, coefficient of variation = 36%), but this variation in MS intake was not associated with milk yield (P > 0·05). The results indicate that the n-alkane and 13C techniques can be successfully combined to estimate the intake of MS and H of grazing cows supplemented as a group. Under the conditions of the present study, individual cows differ considerably in the amount of maize silage consumed per day, although the reasons for this are not clear.

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