Improved analytical methods for the detection and quantification of neutralizing antibodies to biopharmaceuticals

Bioanalysis ◽  
2012 ◽  
Vol 4 (17) ◽  
pp. 2179-2190 ◽  
Author(s):  
Michael G Tovey ◽  
Christophe Lallemand
2009 ◽  
Vol 58 (11) ◽  
pp. 1433-1436 ◽  
Author(s):  
Ali Azizi ◽  
Danylo Sirskyj ◽  
Richard Weltzin ◽  
David E. Anderson ◽  
Francisco Diaz-Mitoma

The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, in vitro assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.


Biologicals ◽  
2019 ◽  
Vol 59 ◽  
pp. 20-28 ◽  
Author(s):  
Jeungwoon Hong ◽  
Daegeun Kim ◽  
Younhee Won ◽  
Jungsoon Yoon ◽  
Kuk Jin Park ◽  
...  

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 13-28 ◽  
Author(s):  
Stephanie L. Bishop ◽  
Susan J. Murch

A review and critical analysis of analytical methods used to quantify the neurotoxin β-N-methylamino-l-alanine in four typically studied sample matrices.


2016 ◽  
Vol 79 (23-24) ◽  
pp. 1615-1624 ◽  
Author(s):  
Reinhard H. H. Neubert ◽  
Olena Bayrak ◽  
Sophie Steinbach ◽  
Stefan Sonnenberger ◽  
Bodo Dobner

2004 ◽  
Vol 1 (2) ◽  
pp. 81 ◽  
Author(s):  
Jacob de Boer

Environmental Context. Flame retardants have been employed for centuries to reduce the flammability of materials; in our age, furniture, textiles, and electronic equipment are heavy users of effective and inexpensive brominated flame retardants. Evidence links these materials to endocrine disruption and bioaccumulation, but better detection and quantification of this large (75 member) molecular family, in order to explore the fine details of this toxic link, is both lacking and of pressing need. Abstract. This paper reviews information on the occurrence of brominated flame retardants (BFRs) in various environmental compartments. The lack of information on BFRs other than pentabrominated diphenylethers (pentaBDEs) is emphasized, as well as the need for better analytical methods and the need for more toxicological information on BFRs. Concern is expressed on the bioaccumulating properties of hexabromocyclododecane (HBCD). It is shown that decaBDE can accumulate in birds of prey, although the levels found are not particularly high. A parallel is drawn with the occurrence of other contaminants that are being found in our environment and foodstuffs, such as polyfluorinated sulphones, phthalates, alkylphenols, and others. These substances play a key role in our modern society, often for our convenience. However, a price may have to be paid by later generations if the consequences of their presence in the environment would be neglected.


Author(s):  
NATILENE MESQUITA BRITO ◽  
OZELITO POSSIDÔNIO DE AMARANTE JUNIOR ◽  
LUCIANA POLESE ◽  
MARIA LÚCIA RIBEIRO

Neste trabalho são apresentadas as definições dos parâmetros (seletividade, limites de detecção e quantificação, exatidão, precisão, linearidade, gráfico analítico, sensibilidade e robustez) considerados nos processos de validação de métodos analíticos. A estratégia a ser adotada para a determinação desses parâmetros depende do propósito e da natureza do método. Exemplos são apresentados para a avaliação dos parâmetros no procedimento de validação. ANALYTICAL METHODS VALIDATION: STRATEGY AND DISCUSSION Abstract This paper presents the definitions of the parameters (selectivity, limits of detection and quantification, accuracy, precision, linearity, analytical graphic, sensitivity and ruggedness) considered on analytical methods validation procedures. The strategy to be adopted for determinations of these parameters depends on the purpose and the nature of the method. Some examples are presented for parameters evaluation in a validation procedure.


2017 ◽  
Vol 1 (1) ◽  
pp. 1-23 ◽  
Author(s):  
Bruna Tassi Borille ◽  
Marina González ◽  
Luiza Steffens ◽  
Rafael Scorsatto Ortiz ◽  
Renata Pereira Limberger

Background: Cannabis has been the most widely used illicit drug worldwide throughout many years. Reports from different countries indicate that the potency of cannabis preparation has been increasing, as well as the ratio of tetrahydrocannabinol/cannabidiol has been changing. The high consumption along with the changing chemical profile of the drug has led increasingly to the interest in researching the cannabis plant. Methods: This article reviews available literature on the analytical methods currently used for the detection and quantification of cannabinoids in cannabis plant. The papers were screened by two researchers independently and following a pre-specified protocol. Results and Discussion: The systematic review of the literature allowed to include 42 citations on cannabis plant analysis. Conclusions: The analytical methods for cannabis material published in the included articles of this systematic review showed a lack of relevant information of the development of methods on GC and LC analysis and the limits of detection and quantification of mass detectors.


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