Prevalence of Anticoagulant Rodenticide Exposure in Red-tailed Hawks (Buteo jamaicensis) in New York State and Diagnostic Utility of Russell Viper Venom Test for Detecting Associated Coagulopathies

Anticoagulant rodenticides (ARs) continue to be used across the United States as a method for controlling unwanted rodent species. As a consequence, wild birds of prey are exposed to toxins by eating poisoned prey items. ARs prevent the hepatic recycling of vitamin K and thereby impede the post-translational processing of coagulation factors II, VII, IX, and X that is required for procoagulant complex assembly. Through this mechanism of action, ARs cause hemorrhage and death in their target species. Various studies have documented the persistence of these contaminants in birds of prey but few have attempted to use affordable and accessible diagnostic tests to diagnose exposure in free-ranging birds of prey. In our study free-ranging red-tailed hawks were found to be exposed to difethialone and brodifacoum. Eleven of sixteen (68%) livers tested for AR exposure were positive. Difethialone was found in 1/16 (6%) liver samples, and brodifacoum was detected in 15/16 (93%) liver samples. Difethialone was found at a concentration of 0.18 ppm and brodifacoum concentrations ranged from 0.003-0.234 ppm. Two out of 34 (6%) RTH assessed for blood rodenticide had brodifacoum in blood with measured concentrations of 0.003 and 0.006 ppm. The range of clotting times in the prothrombin time (PT) and Russell’s viper venom time assays for control RTH were 16.7 to 39.7 seconds and 11.5 to 91.8 seconds, respectively. No correlation was found between PT and RVVT in the control or free-range RTH, and there was no relationship found between the presence of liver anticoagulant residues and clotting times in the PT and RVVT.

Detection and Molecular Characterization of a Novel Species of Circovirus in a Tawny Owl (Strix aluco) in Southern Italy

Thanks to recent developments in molecular methods, many new species have been discovered within the genus Circovirus, which comprises viruses of veterinary relevance found in a broad range of hosts. In particular, several circoviruses are known to infect birds, often causing immunosuppression and feathering disorders. Nonetheless, nothing is known about their circulation in birds of prey. In this study, samples from 61 birds of prey representing ten different species, recovered by a wildlife rescue centre in Southern Italy, were taken at necropsy and analysed by PCR with pan-Circovirus primers. Only one sample, collected from a tawny owl (Strix aluco), tested positive. Its genome, sequenced by primer walking, displays the typical features of circoviruses. Based on demarcation criteria, the detected strain qualifies as a novel species, which was named “tawny owl-associated circovirus” (ToCV). Phylogenetically, ToCV clustered with mammalian rather than avian circoviruses, and its closeness to a rodent circovirus suggests that its host may have been a micromammal eaten by the tawny owl. On the other hand, its detection in the spleen fits with the tropism of other avian circoviruses. Little can be therefore said on its biology and pathogenicity, and further efforts are needed to better characterize its epidemiology.

Burial of a Hunnic Period Noblewoman at Karakabak, Mangystau, Kazakhstan

This study continues a series of publications describing the fi ndings of excavations at the Karakabak cemetery on the Mangyshlak Peninsula, dating to the Hunnic period. Burial 11 was that of a girl dressed in an outfi t imitating a royal vestment. The reconstructed headdress consisted of a cape decorated with round, gold plaques and a diadem-type headband of red cloth with mask-shaped plaques. The central forehead plaque is a replica of Hellenistic gorgoneia. Similar masks were found in the Volga basin and the Northern Black Sea region. Temporal mask-plaques, carved of wood and covered with gold foil, have no parallels but follow the archaic Scythian tradition. Belt and shoe buckles were not attached to belts and were not used in everyday life. In terms of style and technique, the gold casing with an embossed geometric design on a wooden base belongs to a series of artifacts of the so-called Shipovo horizon. The buckle frames are shaped as stylized birds of prey with spread wings. The forehead plaque and details of the shoe straps are paralleled by those from Altynkazgan. The Karakabak artifacts are unique for the Aral-Caspian region, providing yet another indication of close cultural ties with the Hunnic world. All details of the outfi t were likely manufactured at a nearby workshop (the Karakabak settlement) in the second half of the 5th or fi rst half of the 6th century for the burial of a nomadic noblewoman.

Quantifying avian inertial properties using calibrated computed tomography

ABSTRACT Estimating centre of mass and mass moments of inertia is an important aspect of many studies in biomechanics. Characterising these parameters accurately in three dimensions is challenging with traditional methods requiring dissection or suspension of cadavers. Here, we present a method to quantify the three-dimensional centre of mass and inertia tensor of birds of prey using calibrated computed tomography (CT) scans. The technique was validated using several independent methods, providing body segment mass estimates within approximately 1% of physical dissection measurements and moment of inertia measurements with a 0.993 R2 correlation with conventional trifilar pendulum measurements. Calibrated CT offers a relatively straightforward, non-destructive approach that yields highly detailed mass distribution data that can be used for three-dimensional dynamics modelling in biomechanics. Although demonstrated here with birds, this approach should work equally well with any animal or appendage capable of being CT scanned.

Review of deaths and behavior of large birds of prey on power grid facilities in Tatarstan and Udmurtia (Russia), problems of modernization of 6–10 kV overhead power lines

The report analyzes the deaths of large birds of prey on power grid facilities of Tatarstan previously published in the literature since 2012 and additional ones, identified since 2019, including in the neighboring region – Udmurt Republic. Analysis of the data shows that immature Imperial Eagles (Aquila heliaca) up to 3 years old (n=11) died on the 6–10 kV power lines dangerous for birds. The percentage of fledglings that died near breeding territories after leaving their nests was 81.8% (n=9); one bird died in its second year of life and one bird died in its third year. The death of fledglings was identified in 8 breeding areas (in one of them twice), which is 3.9% of all known breeding areas of the Imperial Eagle in Tatarstan by the end of 2021 (n=205) and about 7.2% of 111 breeding areas in 16 administrative districts of southeastern Tatarstan where oil production is taking place. Two out of six fledglings, for which a time interval of death was established, died in the second half of August, and 2 eagles also died in the first and second halves of September. Distances from precisely known nests to locations where the fledglings died ranged from 0.26 to 11.7 km, 2.56 km on average (n=7). In 57.1% of cases deaths occurred at distances less than 1 km (from 260 to 600 m), and in 28.6% of cases at distances from 2 to 3 km. Observations of the behavior of imperial eagles in breeding grounds show a certain selectivity, namely avoidance of the most dangerous power lines. Adaptation of imperial eagles to the electric grid environment continues – 3 new breeding territories on the electric poles of high-voltage power lines were found. Two cases of death of immature White-Tailed Eagles (Haliaeetus albicilla) were identified on 6–10 kV power lines dangerous for birds deep in the forestland on narrow forest cleared strips in Tatarstan and Udmurtia, as well as the Steppe Eagle (Aquila nipalensis) in Udmurtia. Illegal exploitation and even construction of new 6–10 kV power lines dangerous for birds continues. Despite the measures taken to protect birds from death in the electric grid environment, the rate and quality of these measures are such that in the near future power lines will have a negative impact on eagles in the native area as they do now.

Red Spot on the European Green Map: Will the Extra Catastrophic Phenomenon Take the Polish Poaching-Pressured Ospreys to the Brink of Extinction?

Poland is the only European country where the Osprey population is declining due to the mortality of adult birds from poaching, which impacts not only single breeding attempts but also the Lifetime Reproductive Success (LRS) of specimens. However, what if there came an extra mortality factor in the moment of the lowest numbers of Osprey, already vulnerable in the country? In the years 2018–2020, we installed 22 trail cameras and five digital cameras (live online video feeds) on the nests. The total failure level observed in cameras (18.5%) was high. We observed, using these cameras, the extra mortality of chicks (10.7% of potentially fledged chicks) and even adult birds by unexpected predation by Northern Goshawk and White-tailed Eagle. This phenomenon is also common in the national population, as we found a total of ten cases of total losses by predators (eight or nine of them were birds of prey), including nests not covered by camera monitoring. The extra adult-predation by Goshawks means an extra drop in LRS. Those adult and chick predations are an example of exceptional catastrophic phenomena, which have been described as the direct cause of the extinction of animal populations throughout history. Only active conservation and stop poaching of the Polish population could stop the decline and save the Polish Ospreys.