Determination of tamoxifen and endoxifen in dried blood spots using LC–MS/MS and the effect of coated DBS cards on recovery and matrix effects

Bioanalysis ◽  
2014 ◽  
Vol 6 (22) ◽  
pp. 2999-3009 ◽  
Author(s):  
Nynke GL Jager ◽  
Hilde Rosing ◽  
Jan HM Schellens ◽  
Jos H Beijnen
Keyword(s):  
Matrix Effects ◽  
Dried Blood Spots ◽  
Blood Spots

scholarly journals Determination of Antidepressants and Antipsychotics in Dried Blood Spots (DBSs) Collected from Post-Mortem Samples and Evaluation of the Stability over a Three-Month Period

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3636 ◽  
Author(s):  
Matteo Moretti ◽  
Francesca Freni ◽  
Beatrice Valentini ◽  
Claudia Vignali ◽  
Angelo Groppi ◽  
...  
Keyword(s):  
Room Temperature ◽  
Matrix Effects ◽  
Solid Phase ◽  
Dried Blood Spots ◽  
Post Mortem ◽  
Sample Storage ◽  
Blood Spots ◽  
The Stability ◽  
Good Agreement

An LC-MS/MS method for the identification and quantification of antidepressants and antipsychotics was developed on dried blood spots (DBSs). Moreover, analyte stability on DBSs within a 3-month period was monitored. Aliquots of 85 µL of blood from autopsy cases were pipetted onto DBS cards, which were dried and stored at room temperature. DBSs were analyzed in triplicate immediately, within the following 3 weeks, and after 3 months. For each analysis, a whole blood stain was extracted in phosphate buffer and purified using Solid Phase Extraction (SPE) cartridges in order to avoid matrix effects and injected in the LC-MS/MS system. Thirty-nine molecules were screened. Limits of detection (LODs) ranged between 0.1 and 3.2 ng/mL (g) and 0.1 and 5.2 ng/mL (g) for antidepressants and antipsychotics, respectively. Limits of quantification (LOQs) varied from 5 to 10.0 ng/mL for both. Sixteen cases among the 60 analyzed resulted positive for 17 different analytes; for 14 of these the method was fully validated. A general good agreement between the concentrations on DBSs and those measured in conventional blood samples (collected concurrently and stored at −20 °C) was observed. The degradation/enhancement percentage for most of the substances was lower than 20% within the 3-month period. Our results, obtained from real post-mortem cases, suggest that DBSs can be used for routine sample storage.

Determination of Chloroquine in Dried Blood Spots on Filter Paper

1986 ◽  
Vol 8 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Y. Bergqvist ◽  
Ö. Ericsson ◽  
M. Rais
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

A specific LC/ESI-MS/MS method for determination of 25-hydroxyvitamin D3 in neonatal dried blood spots containing a potential interfering metabolite, 3-epi-25-hydroxyvitamin D3

2011 ◽  
Vol 34 (7) ◽  
pp. 725-732 ◽  
Author(s):  
Tatsuya Higashi ◽  
Masahiro Suzuki ◽  
Junji Hanai ◽  
Shinsuke Inagaki ◽  
Jun Zhe Min ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Esi Ms ◽  
Blood Spots

Determination of Fentanyl Analog Exposure Using Dried Blood Spots with LC–MS-MS

2018 ◽  
Vol 43 (4) ◽  
pp. 266-276 ◽  
Author(s):  
Craig Seymour ◽  
Rebecca L Shaner ◽  
Melanie C Feyereisen ◽  
Rebekah E Wharton ◽  
Pearl Kaplan ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots

Determination of psychosine concentration in dried blood spots from newborns that were identified via newborn screening to be at risk for Krabbe disease

2013 ◽  
Vol 419 ◽  
pp. 73-76 ◽  
Author(s):  
Wei-Lien Chuang ◽  
Josh Pacheco ◽  
X. Kate Zhang ◽  
Monica M. Martin ◽  
Chad K. Biski ◽  
...  
Keyword(s):  
At Risk ◽  
Newborn Screening ◽  
Dried Blood Spots ◽  
Krabbe Disease ◽  
Blood Spots

Assessment of DNA contamination from dried blood spots and determination of DNA yield and function using archival newborn dried blood spots

2009 ◽  
Vol 402 (1-2) ◽  
pp. 107-113 ◽  
Author(s):  
Suzanne K. Cordovado ◽  
Marie C. Earley ◽  
Miyono Hendrix ◽  
Rena Driscoll-Dunn ◽  
Michael Glass ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Yield ◽  
Dna Contamination ◽  
And Function

A simple and direct atomic absorption spectrometry method for the direct determination of Hg in dried blood spots and dried urine spots prepared using various microsampling devices

2020 ◽  
Vol 35 (1) ◽  
pp. 136-144 ◽  
Author(s):  
Flávio V. Nakadi ◽  
Raúl Garde ◽  
Márcia A. M. S. da Veiga ◽  
Julio Cruces ◽  
Martín Resano
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Direct Determination ◽  
Absorption Spectrometry ◽  
Direct Analysis ◽  
Dried Blood Spots ◽  
Spectrometry Method ◽  
Blood Spots

Production of dried blood spots and dried urine spots of known volume enables their direct analysis aiming at the fast quantification of Hg.

Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

1980 ◽  
Vol 26 (8) ◽  
pp. 1198-1200 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Filter Paper ◽  
Colorimetric Method ◽  
Dried Blood Spots ◽  
Arginase Activity ◽  
Kinetic Reaction ◽  
Hematological Disorders ◽  
Blood Spots ◽  
Blood Specimens ◽  
The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

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