scholarly journals Determination of Antidepressants and Antipsychotics in Dried Blood Spots (DBSs) Collected from Post-Mortem Samples and Evaluation of the Stability over a Three-Month Period

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3636 ◽  
Author(s):  
Matteo Moretti ◽  
Francesca Freni ◽  
Beatrice Valentini ◽  
Claudia Vignali ◽  
Angelo Groppi ◽  
...  
Keyword(s):  
Room Temperature ◽  
Matrix Effects ◽  
Solid Phase ◽  
Dried Blood Spots ◽  
Post Mortem ◽  
Sample Storage ◽  
Blood Spots ◽  
The Stability ◽  
Good Agreement

An LC-MS/MS method for the identification and quantification of antidepressants and antipsychotics was developed on dried blood spots (DBSs). Moreover, analyte stability on DBSs within a 3-month period was monitored. Aliquots of 85 µL of blood from autopsy cases were pipetted onto DBS cards, which were dried and stored at room temperature. DBSs were analyzed in triplicate immediately, within the following 3 weeks, and after 3 months. For each analysis, a whole blood stain was extracted in phosphate buffer and purified using Solid Phase Extraction (SPE) cartridges in order to avoid matrix effects and injected in the LC-MS/MS system. Thirty-nine molecules were screened. Limits of detection (LODs) ranged between 0.1 and 3.2 ng/mL (g) and 0.1 and 5.2 ng/mL (g) for antidepressants and antipsychotics, respectively. Limits of quantification (LOQs) varied from 5 to 10.0 ng/mL for both. Sixteen cases among the 60 analyzed resulted positive for 17 different analytes; for 14 of these the method was fully validated. A general good agreement between the concentrations on DBSs and those measured in conventional blood samples (collected concurrently and stored at −20 °C) was observed. The degradation/enhancement percentage for most of the substances was lower than 20% within the 3-month period. Our results, obtained from real post-mortem cases, suggest that DBSs can be used for routine sample storage.

Determination of benzodiazepines in blood and in dried blood spots collected from post‐mortem samples and evaluation of the stability over a three‐month period

2019 ◽  
Vol 11 (9) ◽  
pp. 1403-1411 ◽  
Author(s):  
Matteo Moretti ◽  
Francesca Freni ◽  
Ilaria Tomaciello ◽  
Claudia Vignali ◽  
Angelo Groppi ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Post Mortem ◽  
Blood Spots ◽  
The Stability

Fluorometric micromethod for determination of arginase activity in dried blood spots on filter paper.

1980 ◽  
Vol 26 (8) ◽  
pp. 1198-1200 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Filter Paper ◽  
Colorimetric Method ◽  
Dried Blood Spots ◽  
Arginase Activity ◽  
Kinetic Reaction ◽  
Hematological Disorders ◽  
Blood Spots ◽  
Blood Specimens ◽  
The Stability

Abstract We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.

Determination of tamoxifen and endoxifen in dried blood spots using LC–MS/MS and the effect of coated DBS cards on recovery and matrix effects

Bioanalysis ◽  
2014 ◽  
Vol 6 (22) ◽  
pp. 2999-3009 ◽  
Author(s):  
Nynke GL Jager ◽  
Hilde Rosing ◽  
Jan HM Schellens ◽  
Jos H Beijnen
Keyword(s):  
Matrix Effects ◽  
Dried Blood Spots ◽  
Blood Spots

Determination of Chloroquine in Dried Blood Spots on Filter Paper

1986 ◽  
Vol 8 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Y. Bergqvist ◽  
Ö. Ericsson ◽  
M. Rais
Keyword(s):  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

A specific LC/ESI-MS/MS method for determination of 25-hydroxyvitamin D3 in neonatal dried blood spots containing a potential interfering metabolite, 3-epi-25-hydroxyvitamin D3

2011 ◽  
Vol 34 (7) ◽  
pp. 725-732 ◽  
Author(s):  
Tatsuya Higashi ◽  
Masahiro Suzuki ◽  
Junji Hanai ◽  
Shinsuke Inagaki ◽  
Jun Zhe Min ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Esi Ms ◽  
Blood Spots

Impact of sulfate on the solubility of Tc(IV) in acidic to hyperalkaline aqueous reducing systems

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Sarah B. Duckworth ◽  
Xavier Gaona ◽  
Alexander Baumann ◽  
Kathy Dardenne ◽  
Jörg Rothe ◽  
...  
Keyword(s):  
Solid Phase ◽  
Correction Factors ◽  
Mixed Salt ◽  
Ph Values ◽  
Reducing Conditions ◽  
Upper Limits ◽  
Salt Systems ◽  
Amorphous Character ◽  
Good Agreement

Abstract The solubility of 99Tc(IV) was investigated from undersaturation conditions in NaCl–Na2SO4 (0.3 M ≤ I ≤ 5.0 M), MgCl2–MgSO4 (I = 13.5 M) and CaCl2–CaSO4 (I = 13.5 M) systems with 0.001 M ≤ [SO4 2−]tot ≤ 1.0 M and 1 ≤ pH m  ≤ 12 (with pH m  = −log[H+], in molal units). Reducing conditions were set by either Sn(II) or Fe(0). Special efforts were dedicated to accurately characterize the correction factors A m required for the determination of pH m from the experimentally measured pH values in the mixed salt systems investigated, with pH m  = pHexp + A m . The combination of (pe + pH m ) measurements with Pourbaix diagrams of Tc suggests that technetium is present in its +IV redox state. This hypothesis is confirmed by XANES, which unambiguously shows the predominance of Tc(IV) both in the aqueous and solid phases of selected solubility samples. XRD and SEM–EDS support the amorphous character of the solid phase controlling the solubility of Tc(IV). EXAFS data confirm the predominance of TcO2(am, hyd) at pH m  > 1.5, whereas the formation of a Tc(IV)–O–Cl solid phase is hinted at lower pHm values in concentrated NaCl–Na2SO4 systems with ≈5 M NaCl. Solubility data collected in sulfate-containing systems are generally in good agreement with previous solubility studies conducted in sulfate-free NaCl, MgCl2 and CaCl2 solutions of analogous ionic strength. Although the complexation of Tc(IV) with sulfate cannot be completely ruled out, these results strongly support that, if occurring, complexation must be weak and has no significant impact on the solubility of Tc(IV) in dilute up to highly saline media. Solubility upper-limits determined in this work can be used for source term estimations including the effect of sulfate in a variety of geochemical conditions relevant in the context of nuclear waste disposal.

Determination of Fentanyl Analog Exposure Using Dried Blood Spots with LC–MS-MS

2018 ◽  
Vol 43 (4) ◽  
pp. 266-276 ◽  
Author(s):  
Craig Seymour ◽  
Rebecca L Shaner ◽  
Melanie C Feyereisen ◽  
Rebekah E Wharton ◽  
Pearl Kaplan ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots

Determination of psychosine concentration in dried blood spots from newborns that were identified via newborn screening to be at risk for Krabbe disease

2013 ◽  
Vol 419 ◽  
pp. 73-76 ◽  
Author(s):  
Wei-Lien Chuang ◽  
Josh Pacheco ◽  
X. Kate Zhang ◽  
Monica M. Martin ◽  
Chad K. Biski ◽  
...  
Keyword(s):  
At Risk ◽  
Newborn Screening ◽  
Dried Blood Spots ◽  
Krabbe Disease ◽  
Blood Spots

Assessment of DNA contamination from dried blood spots and determination of DNA yield and function using archival newborn dried blood spots

2009 ◽  
Vol 402 (1-2) ◽  
pp. 107-113 ◽  
Author(s):  
Suzanne K. Cordovado ◽  
Marie C. Earley ◽  
Miyono Hendrix ◽  
Rena Driscoll-Dunn ◽  
Michael Glass ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Yield ◽  
Dna Contamination ◽  
And Function
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