Current advances in the development of high-throughput purification strategies for the generation of therapeutic antibodies

2015 ◽  
Vol 3 (6) ◽  
pp. 411-424 ◽  
Author(s):  
Jennifer Spooner ◽  
Trevor Wilkinson ◽  
Benjamin P Kemp
Keyword(s):  
High Throughput ◽  
Therapeutic Antibodies

scholarly journals A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 517
Author(s):  
Seohee Chang ◽  
Soohyun Kim ◽  
Jerome Han ◽  
Suji Ha ◽  
Hyunho Lee ◽  
...  
Keyword(s):  
High Throughput ◽  
Retrieval System ◽  
Low Cost ◽  
Therapeutic Antibodies ◽  
Amino Acid Residues ◽  
Single Chain ◽  
Scfv Library ◽  
Single Clone ◽  
Library Complexity ◽  
Lower Complexity

Phage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low cost, it has intrinsic technical limitations, such as the asymmetrical amplification of the library after each round of biopanning, which is regarded as a reason for it yielding a very limited number of antigen binders. In this study, we developed a high-throughput single-clonal screening system comprised of fluorescence immunoassays and a laser-driven clonal DNA retrieval system using microchip technology. Using this system, from a single-chain variable fragment (scFv) library displayed on phages with a complexity of 5.21 × 105 harboring random mutations at five amino acid residues, more than 70,000 clones—corresponding to ~14% of the library complexity—were screened, resulting in 78 antigen-reactive scFv sequences with mutations restricted to the randomized residues. Our results demonstrate that this system can significantly reduce the number of biopanning rounds, or even eliminate the need for this process for libraries with lower complexity, providing an opportunity to obtain more diverse clones from the library.

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
Sign in / Sign up

Export Citation Format

Share Document