High-throughput profiling of N-linked oligosaccharides in therapeutic antibodies using a microfluidic CD platform and MALDI-MS

2010 ◽  
Vol 399 (4) ◽  
pp. 1601-1611 ◽  
Author(s):  
Tran Thi Thuy ◽  
Mats Inganäs ◽  
Gunnar Thorsén
Keyword(s):  
High Throughput ◽  
Maldi Ms ◽  
Therapeutic Antibodies

MassyTools: A High-Throughput Targeted Data Processing Tool for Relative Quantitation and Quality Control Developed for Glycomic and Glycoproteomic MALDI-MS

2015 ◽  
Vol 14 (12) ◽  
pp. 5088-5098 ◽  
Author(s):  
Bas C. Jansen ◽  
Karli R. Reiding ◽  
Albert Bondt ◽  
Agnes L. Hipgrave Ederveen ◽  
Magnus Palmblad ◽  
...  
Keyword(s):  
Quality Control ◽  
Data Processing ◽  
High Throughput ◽  
Maldi Ms ◽  
Relative Quantitation ◽  
Data Processing Tool

High-throughput screening and spatial profiling of low-mass pesticides using a novel Ti3C2 MXene nanowire (TMN) as MALDI MS matrix

Chemosphere ◽  
2021 ◽  
pp. 131826
Author(s):  
Chunxia Ma ◽  
Xiao Wang ◽  
Huamin Zhang ◽  
Wei Liu ◽  
Daijie Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Maldi Ms ◽  
Spatial Profiling ◽  
Low Mass

scholarly journals Integration of an In Situ MALDI-Based High-Throughput Screening Process: A Case Study with Receptor Tyrosine Kinase c-MET

2017 ◽  
Vol 22 (10) ◽  
pp. 1203-1210 ◽  
Author(s):  
Katrin Beeman ◽  
Jens Baumgärtner ◽  
Manuel Laubenheimer ◽  
Karlheinz Hergesell ◽  
Martin Hoffmann ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Maldi Ms ◽  
Kinase Assay ◽  
Label Free ◽  
Screening Process ◽  
Label Free Detection ◽  
Ic50 Values

Mass spectrometry (MS) is known for its label-free detection of substrates and products from a variety of enzyme reactions. Recent hardware improvements have increased interest in the use of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS for high-throughput drug discovery. Despite interest in this technology, several challenges remain and must be overcome before MALDI-MS can be integrated as an automated “in-line reader” for high-throughput drug discovery. Two such hurdles include in situ sample processing and deposition, as well as integration of MALDI-MS for enzymatic screening assays that usually contain high levels of MS-incompatible components. Here we adapt our c-MET kinase assay to optimize for MALDI-MS compatibility and test its feasibility for compound screening. The pros and cons of the Echo (Labcyte) as a transfer system for in situ MALDI-MS sample preparation are discussed. We demonstrate that this method generates robust data in a 1536-grid format. We use the MALDI-MS to directly measure the ratio of c-MET substrate and phosphorylated product to acquire IC50 curves and demonstrate that the pharmacology is unaffected. The resulting IC50 values correlate well between the common label-based capillary electrophoresis and the label-free MALDI-MS detection method. We predict that label-free MALDI-MS-based high-throughput screening will become increasingly important and more widely used for drug discovery.

scholarly journals MALDIViz: A Comprehensive Informatics Tool for MALDI-MS Data Visualization and Analysis

2017 ◽  
Vol 22 (10) ◽  
pp. 1246-1252 ◽  
Author(s):  
Kishore Kumar Jagadeesan ◽  
Simon Ekström
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
High Throughput Screening ◽  
Web Application ◽  
Low Cost ◽  
Maldi Ms ◽  
Ionization Mass ◽  
Label Free ◽  
Label Free Detection ◽  
R Shiny

Recently, mass spectrometry (MS) has emerged as an important tool for high-throughput screening (HTS) providing a direct and label-free detection method, complementing traditional fluorescent and colorimetric methodologies. Among the various MS techniques used for HTS, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides many of the characteristics required for high-throughput analyses, such as low cost, speed, and automation. However, visualization and analysis of the large datasets generated by HTS MALDI-MS can pose significant challenges, especially for multiparametric experiments. The datasets can be generated fast, and the complexity of the experimental data (e.g., screening many different sorbent phases, the sorbent mass, and the load, wash, and elution conditions) makes manual data analysis difficult. To address these challenges, a comprehensive informatics tool called MALDIViz was developed. This tool is an R-Shiny-based web application, accessible independently of the operating system and without the need to install any program locally. It has been designed to facilitate easy analysis and visualization of MALDI-MS datasets, comparison of multiplex experiments, and export of the analysis results to high-quality images.

scholarly journals A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 517
Author(s):  
Seohee Chang ◽  
Soohyun Kim ◽  
Jerome Han ◽  
Suji Ha ◽  
Hyunho Lee ◽  
...  
Keyword(s):  
High Throughput ◽  
Retrieval System ◽  
Low Cost ◽  
Therapeutic Antibodies ◽  
Amino Acid Residues ◽  
Single Chain ◽  
Scfv Library ◽  
Single Clone ◽  
Library Complexity ◽  
Lower Complexity

Phage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low cost, it has intrinsic technical limitations, such as the asymmetrical amplification of the library after each round of biopanning, which is regarded as a reason for it yielding a very limited number of antigen binders. In this study, we developed a high-throughput single-clonal screening system comprised of fluorescence immunoassays and a laser-driven clonal DNA retrieval system using microchip technology. Using this system, from a single-chain variable fragment (scFv) library displayed on phages with a complexity of 5.21 × 105 harboring random mutations at five amino acid residues, more than 70,000 clones—corresponding to ~14% of the library complexity—were screened, resulting in 78 antigen-reactive scFv sequences with mutations restricted to the randomized residues. Our results demonstrate that this system can significantly reduce the number of biopanning rounds, or even eliminate the need for this process for libraries with lower complexity, providing an opportunity to obtain more diverse clones from the library.

scholarly journals High-throughput glycopeptide profiling of prostate-specific antigen from seminal plasma by MALDI-MS

Talanta ◽  
2021 ◽  
Vol 222 ◽  
pp. 121495
Author(s):  
Wei Wang ◽  
Anna Kałuża ◽  
Jan Nouta ◽  
Simone Nicolardi ◽  
Mirosława Ferens-Sieczkowska ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
High Throughput ◽  
Seminal Plasma ◽  
Specific Antigen ◽  
Maldi Ms

Label-Free High-Throughput Screening Assay for Inhibitors of Alzheimer’s Amyloid-β Peptide Aggregation Based on MALDI MS

2010 ◽  
Vol 82 (20) ◽  
pp. 8558-8565 ◽  
Author(s):  
Kairit Zovo ◽  
Eneken Helk ◽  
Ann Karafin ◽  
Vello Tõugu ◽  
Peep Palumaa
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Amyloid Β ◽  
Maldi Ms ◽  
Amyloid Β Peptide ◽  
Peptide Aggregation ◽  
Label Free ◽  
Screening Assay ◽  
High Throughput Screening Assay

Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

2014 ◽  
Vol 50 (90) ◽  
pp. 13960-13962 ◽  
Author(s):  
Zhenzhen Deng ◽  
Mingliang Ye ◽  
Yangyang Bian ◽  
Zheyi Liu ◽  
Fangjie Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Kinase Activity ◽  
Maldi Ms ◽  
Time Dependent ◽  
Activity Assay ◽  
Dimethyl Labeling ◽  
Labeling Approach ◽  
Kinase Activity Assay

A multiplex isotope dimethyl labeling approach allowed MALDI MS to monitor the time dependent consumption of substrates and generation of products in one spot.

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