Different expression of miRNAs targeting helicases in rice in response to low and high dose rate γ-ray treatments

The positions of the components of a reactor can change over time, due to radiation damage, sagging, etc. Thus, it is important to determine their positions. To satisfy this requirement of the staff at the Point Lepreau Generating Station, a method to determine the positions of reactor components has been developed and demonstrated. This method combines the use of dose rate measurements and Monte Carlo simulations. It first involves measuring the high γ-ray dose rates as a function of position within a reactor. Then it entails comparing these measurements with Monte Carlo simulations. In order to perform such measurements, a silicon diode detector and a scan drive system have been developed. In 2009, measurements of the γ-ray dose rate profile of the shut down Point Lepreau Generating Station reactor were conducted. By comparing the locations of the local peaks in the dose rate data, it was possible to determine the distances between the steel reactor components. The measured data were then compared with Monte Carlo simulations to determine how precisely one could locate the positions of the adjuster rods. Using this technique, it was found that the retracted adjuster rods were 440 ± 60 mm below their designed positions.

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Diminished or inversed dose-rate effect on clonogenic ability in Ku-deficient rodent cells

Journal of Radiation Research ◽

10.1093/jrr/rraa128 ◽

2020 ◽

Author(s):

Hisayo Tsuchiya ◽

Mikio Shimada ◽

Kaima Tsukada ◽

Qingmei Meng ◽

Junya Kobayashi ◽

...

Keyword(s):

Cell Survival ◽

Dose Rate ◽

Biological Effects ◽

Severe Combined Immunodeficiency ◽

High Dose Rate ◽

Rate Effect ◽

High Dose ◽

Γ Ray ◽

Clonogenic Cell ◽

Dose Rate Effect

Abstract The biological effects of ionizing radiation, especially those of sparsely ionizing radiations like X-ray and γ-ray, are generally reduced as the dose rate is reduced. This phenomenon is known as ‘the dose-rate effect’. The dose-rate effect is considered to be due to the repair of DNA damage during irradiation but the precise mechanisms for the dose-rate effect remain to be clarified. Ku70, Ku86 and DNA-dependent protein kinase catalytic subunit (DNA-PKcs) are thought to comprise the sensor for DNA double-strand break (DSB) repair through non-homologous end joining (NHEJ). In this study, we measured the clonogenic ability of Ku70-, Ku86- or DNA-PKcs-deficient rodent cells, in parallel with respective control cells, in response to high dose-rate (HDR) and low dose-rate (LDR) γ-ray radiation (~0.9 and ~1 mGy/min, respectively). Control cells and murine embryonic fibroblasts (MEF) from a severe combined immunodeficiency (scid) mouse, which is DNA-PKcs-deficient, showed higher cell survival after LDR irradiation than after HDR irradiation at the same dose. On the other hand, MEF from Ku70−/− mice exhibited lower clonogenic cell survival after LDR irradiation than after HDR irradiation. XR-V15B and xrs-5 cells, which are Ku86-deficient, exhibited mostly identical clonogenic cell survival after LDR and HDR irradiation. Thus, the dose-rate effect in terms of clonogenic cell survival is diminished or even inversed in Ku-deficient rodent cells. These observations indicate the involvement of Ku in the dose-rate effect.

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