Adipose-derived stem cell conditioned media extends survival of a mouse model of amyotrophic lateral sclerosis

2012 ◽  
Vol 01 (S1) ◽  
Author(s):  
Yansheng Du
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Stem Cell ◽  
Mouse Model ◽  
Conditioned Media ◽  
Adipose Derived Stem Cell ◽  
Lateral Sclerosis

scholarly journals Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Christine V. Fontanilla ◽  
Huiying Gu ◽  
Qingpeng Liu ◽  
Timothy Z. Zhu ◽  
Changwei Zhou ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Mouse Model ◽  
Survival Time ◽  
Neuronal Death ◽  
Immunohistochemical Analysis ◽  
Mitogen Activated Protein Kinase ◽  
Trophic Factors ◽  
Mitogen Activated Protein ◽  
Adipose Derived Stem Cell ◽  
Lateral Sclerosis

Abstract Adipose stromal cells (ASC) secrete various trophic factors that assist in the protection of neurons in a variety of neuronal death models. In this study, we tested the effects of human ASC conditional medium (ASC-CM) in human amyotrophic lateral sclerosis (ALS) transgenic mouse model expressing mutant superoxide dismutase (SOD1G93A). Treating symptomatic SOD1G93A mice with ASC-CM significantly increased post-onset survival time and lifespan. Moreover, SOD1G93A mice given ASC-CM treatment showed high motor neuron counts, less activation of microglia and astrocytes at an early symptomatic stage in the spinal cords under immunohistochemical analysis. SOD1G93A mice treated with ASC-CM for 7 days showed reduced levels of phosphorylated p38 (pp38) in the spinal cord, a mitogen-activated protein kinase that is involved in both inflammation and neuronal death. Additionally, the levels of α-II spectrin in spinal cords were also inhibited in SOD1G93A mice treated with ASC-CM for 3 days. Interestingly, nerve growth factor (NGF), a neurotrophic factor found in ASC-CM, played a significant role in the protection of neurodegeneration inSOD1G93A mouse. These results indicate that ASC-CM has the potential to develop into a novel and effective therapeutic treatment for ALS.

scholarly journals Serial in vivo imaging of transplanted allogeneic neural stem cell survival in a mouse model of amyotrophic lateral sclerosis

2017 ◽  
Vol 289 ◽  
pp. 96-102 ◽  
Author(s):  
Amit K. Srivastava ◽  
Sarah K. Gross ◽  
Akshata A. Almad ◽  
Camille A. Bulte ◽  
Nicholas J. Maragakis ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Stem Cell ◽  
Mouse Model ◽  
Cell Survival ◽  
Neural Stem Cell ◽  
In Vivo Imaging ◽  
Stem Cell Survival ◽  
Lateral Sclerosis

Adipose-derived stem cell exosomes alleviate pathology of amyotrophic lateral sclerosis in vitro

2016 ◽  
Vol 479 (3) ◽  
pp. 434-439 ◽  
Author(s):  
Mijung Lee ◽  
Jae-Jun Ban ◽  
Ki Yoon Kim ◽  
Gye Sun Jeon ◽  
Wooseok Im ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Stem Cell ◽  
Adipose Derived Stem Cell ◽  
Lateral Sclerosis
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