scholarly journals A Species-Specific PCR Based Assay for Rapid Detection of Mango Anthracnose Pathogen Colletotrichum gloeosporioides Penz. and Sacc

2013 ◽  
Vol 04 (06) ◽  
Author(s):  
M Kamle
Keyword(s):  
Rapid Detection ◽  
Colletotrichum Gloeosporioides ◽  
Specific Pcr ◽  
Species Specific

Molecular and morphological markers for rapid distinction between 2 Colletotrichum species

2009 ◽  
Vol 55 (9) ◽  
pp. 1076-1088 ◽  
Author(s):  
Sônia Alvim Veiga Pileggi ◽  
Sarah Franco Vieira de Oliveira ◽  
Caroline Waculicz Andrade ◽  
Vânia Aparecida Vicente ◽  
Patrícia do Rocio Dalzoto ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Colletotrichum Gloeosporioides ◽  
Sequence Data ◽  
Morphological Markers ◽  
Antimicrobial Substances ◽  
Specific Pcr ◽  
Endophytic Microorganisms ◽  
Species Specific ◽  
First Time ◽  
Maytenus Ilicifolia

Endophytic microorganisms reside asymptomatically within plants and are a source of new bioactive products for use in medicine, agriculture, and industry. Colletotrichum (teleomorph Glomerella ) is a fungus widely cited in the literature as a producer of antimicrobial substances. Identification at the species level, however, has been a problem in this type of study. Several authors have reported the presence of endophytic fungi from the medicinal plant Maytenus ilicifolia (“espinheira-santa”) in Brazil that has antimicrobial activity against various pathogens. Therefore, Colletotrichum strains were isolated from M. ilicifolia and identified based on morphology, RAPD markers, sequence data of the internal transcribed spacer regions (ITS-1 and ITS-2), the 5.8S gene, and species-specific PCR. The analyses suggested the presence of 2 species, Colletotrichum gloeosporioides and Colletotrichum boninense . Two morphological markers were characterized to allow C. gloeosporioides and C. boninense to be distinguished quickly and accurately. The molecular diagnosis of C. boninense was confirmed by using Col1 and ITS4 primers. This species of Colletotrichum is reported for the first time in M. ilicifolia.

Rapid detection of fraudulence in seven commercial shrimp products by species-specific PCR assays

Food Control ◽  
2021 ◽  
Vol 124 ◽  
pp. 107871
Author(s):  
Lidiya Wilwet ◽  
Robinson Jeya Shakila ◽  
Balasubramanian Sivaraman ◽  
Binaya Bhusan Nayak ◽  
H. Sanath Kumar ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Pcr Assays ◽  
Species Specific

Rapid detection and identification of Streptococcus ratti by a species-specific PCR method

Anaerobe ◽  
2012 ◽  
Vol 18 (1) ◽  
pp. 44-47
Author(s):  
Jumpei Nishio ◽  
Makoto Taniguchi ◽  
Juichiro Higashi ◽  
Masakazu Takahashi ◽  
Takuma Ando ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Detection And Identification ◽  
Pcr Method ◽  
Species Specific ◽  
Streptococcus Ratti

Species-specific PCR-based marker for rapid detection of Aspidiotus rigidus Reyne (Hemiptera: Diaspididae)

2022 ◽  
Vol 25 (1) ◽  
pp. 101848
Author(s):  
Romnick A. Latina ◽  
Darlon V. Lantican ◽  
Michelle S. Guerrero ◽  
Edsel C. Rubico ◽  
Janice F. Laquinta ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Species Specific

Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation

2003 ◽  
Vol 81 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Marina Papadelli ◽  
Eugenia Manolopoulou ◽  
George Kalantzopoulos ◽  
Effie Tsakalidou
Keyword(s):  
Rapid Detection ◽  
Specific Pcr ◽  
Detection And Identification ◽  
Dna Hybridisation ◽  
Species Specific

scholarly journals Survey on Perkinsus Species in Manila Clam Ruditapes philippinarum in Korean Waters Using Species-Specific PCR

2017 ◽  
Vol 52 (4) ◽  
pp. 202-205 ◽  
Author(s):  
Hyun-Sil Kang ◽  
Hyun-Sung Yang ◽  
Kimberly S. Reece ◽  
Young-Ghan Cho ◽  
Hye-Mi Lee ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Korean Waters ◽  
Specific Pcr ◽  
Species Specific

scholarly journals Development and Validation of a Nested-PCR-Denaturing Gradient Gel Electrophoresis Method for Taxonomic Characterization of Bifidobacterial Communities

2003 ◽  
Vol 69 (11) ◽  
pp. 6380-6385 ◽  
Author(s):  
R. Temmerman ◽  
L. Masco ◽  
T. Vanhoutte ◽  
G. Huys ◽  
J. Swings
Keyword(s):  
Nested Pcr ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Temporal Changes ◽  
Rrna Gene ◽  
Specific Pcr ◽  
Gradient Gel Electrophoresis ◽  
Species Specific ◽  
Taxonomic Characterization

ABSTRACT The taxonomic characterization of a bacterial community is difficult to combine with the monitoring of its temporal changes. None of the currently available identification techniques are able to visualize a “complete” community, whereas techniques designed for analyzing bacterial ecosystems generally display limited or labor-intensive identification potential. This paper describes the optimization and validation of a nested-PCR-denaturing gradient gel electrophoresis (DGGE) approach for the species-specific analysis of bifidobacterial communities from any ecosystem. The method comprises a Bifidobacterium-specific PCR step, followed by purification of the amplicons that serve as template DNA in a second PCR step that amplifies the V3 and V6-V8 regions of the 16S rRNA gene. A mix of both amplicons is analyzed on a DGGE gel, after which the band positions are compared with a previously constructed database of reference strains. The method was validated through the analysis of four artificial mixtures, mimicking the possible bifidobacterial microbiota of the human and chicken intestine, a rumen, and the environment, and of two fecal samples. Except for the species Bifidobacterium coryneforme and B. indicum, all currently known bifidobacteria originating from various ecosystems can be identified in a highly reproducible manner. Because no further cloning and sequencing of the DGGE bands is necessary, this nested-PCR-DGGE technique can be completed within a 24-h span, allowing the species-specific monitoring of temporal changes in the bifidobacterial community.

Sign in / Sign up

Export Citation Format

Share Document