In vitro Antifungal Susceptibility of Candida albicans Isolates from Patients with Chronic Periodontitis and Diabetes

Background: Vulvovaginal candidiasis (VVC) is the most frequent fungal disorder in healthy and normal women. Objectives: The aim of this study was to evaluate the in vitro antifungal susceptibility of clinical isolates Candida albicans and Candida glabrata, the two most common candida species in Iranian patients with VVC. Methods: One hundred and eight clinical isolates of candida, including; C. albicans (n = 77) and C. glabrata: (n = 31) were isolated from the 108 patients with VVC. The in vitro activity of caspofungin (CAS), amphotericin B (AMB), voriconazole (VRC), itraconazole (ITC), fluconazole (FLC), and nystatin (NYS) were determined according to the CLSI M27-A3 and CLSI M27-S4. Results: Our results were shown 8 (25.8 %) and 6 (7.8 %) C. glabrata and C. albicans isolates resistance to FLU, respectively. Furthermore, resistance to VRC and ITC were observed in 8.4%, and 3.7% of all isolates, and six isolates (5.6%) had intermediate MIC to CAS. Conclusions: We reported 8 (25.8 %) and 6 (7.8 %) C. glabrata and C. albicans isolates resistance to FLU, respectively. Furthermore, resistance to VRC and ITC were observed in 8.4% and 3.7% of all isolates, respectively.

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Virulence and in vitro antifungal susceptibility of Candida albicans and Candida catenulata from laying hens

International Microbiology ◽

10.1007/s10123-020-00141-1 ◽

2020 ◽

Author(s):

Wafa Rhimi ◽

Chioma Inyang Aneke ◽

Giada Annoscia ◽

Antonio Camarda ◽

Adriana Mosca ◽

...

Keyword(s):

Candida Albicans ◽

Laying Hens ◽

Antifungal Susceptibility ◽

In Vitro Antifungal Susceptibility

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In vitro antifungal susceptibility of Candida spp. isolates from patients with chronic periodontitis and from control patients

Brazilian Oral Research ◽

10.1590/s1806-83242004000100015 ◽

2004 ◽

Vol 18 (1) ◽

pp. 80-84 ◽

Cited By ~ 11

Author(s):

Cristiane Yumi Koga Ito ◽

Clélia Aparecida de Paiva Martins ◽

Jussara Cia Sanches Loberto ◽

Silvana Soléo Ferreira dos Santos ◽

Antonio Olavo Cardoso Jorge

Keyword(s):

Systemic Therapy ◽

Chronic Periodontitis ◽

Antifungal Susceptibility ◽

Antifungal Drugs ◽

Control Group ◽

Immunocompromised Patients ◽

Candida Spp ◽

In Vitro Antifungal Susceptibility ◽

Group 1

Superinfection by Candida can be refractory to conventional periodontal treatments in specific situations, such as in immunocompromised patients. In these cases, the systemic therapy with antifungal drugs could be indicated. The aim of this study was to analyse antifungal susceptibility of Candida spp. strains isolated from chronic periodontitis patients and from control individuals. A total of 39 C. albicans isolates, 9 C. tropicalis, 2 C. glabrata and 5 Candida spp. from control individuals and 30 C. albicans, 3 C. tropicalis and 2 C. glabrata from periodontitis patients were tested. In the control group, 1 isolate of C. glabrata was resistant to ketoconazole and 1 Candida spp. was resistant to amphotericin B, ketoconazole and miconazole. Among the isolates of periodontitis group, 1 (3.33%) C. albicans isolate was resistant to flucytosine and ketoconazole. According to the obtained results, it could be concluded that fluconazole was the most effective drug against the several Candida species studied. There were not expressive differences in the susceptibility of isolates from periodontitis patients or from control individuals.

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Molecular Epidemiology and Antifungal Susceptibility of Trichophyton Isolates in Greece: Emergence of Terbinafine-Resistant Trichophytonmentagrophytes Type VIII Locally and Globally

Journal of Fungi ◽

10.3390/jof7060419 ◽

2021 ◽

Vol 7 (6) ◽

pp. 419

Author(s):

Maria Siopi ◽

Ioanna Efstathiou ◽

Konstantinos Theodoropoulos ◽

Spyros Pournaras ◽

Joseph Meletiadis

Keyword(s):

Molecular Epidemiology ◽

Antifungal Susceptibility ◽

Reference Method ◽

Cross Resistance ◽

Squalene Epoxidase ◽

In Vitro Susceptibility ◽

Type Viii ◽

In Vitro Antifungal Susceptibility ◽

Resistance Rates

Trichophyton isolates with reduced susceptibility to antifungals are now increasingly reported worldwide. We therefore studied the molecular epidemiology and the in vitro antifungal susceptibility patterns of Greek Trichophyton isolates over the last 10 years with the newly released EUCAST reference method for dermatophytes. Literature was reviewed to assess the global burden of antifungal resistance in Trichophyton spp. The in vitro susceptibility of 112 Trichophyton spp. molecularly identified clinical isolates (70 T. rubrum, 24 T. mentagrophytes, 12 T. interdigitale and 6 T. tonsurans) was tested against terbinafine, itraconazole, voriconazole and amorolfine (EUCAST E.DEF 11.0). Isolates were genotyped based on the internal transcribed spacer (ITS) sequences and the target gene squalene epoxidase (SQLE) was sequenced for isolates with reduced susceptibility to terbinafine. All T. rubrum, T. interdigitale and T. tonsurans isolates were classified as wild-type (WT) to all antifungals, whereas 9/24 (37.5%) T. mentagrophytes strains displayed elevated terbinafine MICs (0.25–8 mg/L) but not to azoles and amorolfine. All T. interdigitale isolates belonged to ITS Type II, while T. mentagrophytes isolates belonged to ITS Type III* (n = 11), VIII (n = 9) and VII (n = 4). All non-WT T. mentagrophytes isolates belonged to Indian Genotype VIII and harbored Leu393Ser (n = 5) and Phe397Leu (n = 4) SQLE mutations. Terbinafine resistance rates ranged globally from 0–44% for T. rubrum and 0–76% for T. interdigitale/T. mentagrophytes with strong endemicity. High incidence (37.5%) of terbinafine non-WT T. mentagrophytes isolates (all belonging to ITS Type VIII) without cross-resistance to other antifungals was found for the first time in Greece. This finding must alarm for susceptibility testing of dermatophytes at a local scale particularly in non-responding dermatophytoses.

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