Almohisen A. Ibrahem (2020). Genetic variability and population structure of
Saudi Arabia bread wheat (Triticum aestivum L.) by microsatellite markers-
Genetika, Vol 52, No.3, 943-956. Wheat (Triticum aestivum L.) is an
important cereal crop. Analysis of genetic diversity and population
structure in local landraces would improve the wheat breeding program by
more efficient use of genetic materials and management of genetic variation.
To address this challenge, a set of thirteen Saudi Arabia wheat landraces
was used to assess population structure and genetic diversity. Thirteen
landraces were genotyped using eighteen microsatellite markers which
revealed a clear polymorphism among these genotypes. In total, 136 alleles
from a set of eighteen simple sequence repeats (SSRs) loci on a panel of
thirteen wheat landraces were detected. All SSRs loci showed a wide range of
allele numbers extended from 3 to 11 alleles with an average of 7.5. Genetic
diversity, polymorphism information content and minor allele frequency
ranged from 0.26 to 0.50, 0.23 to 0.37 and 0.15 to 0.46 with an average
0.43, 0.33 and 0.34, respectively. The results of principal coordinate
analysis (PCoA) agreed with the structure analysis. Structure grouped the
thirteen landraces into three clear subpopulations. The fixation index
(Fst), a measure of population substructure, was 0.217, 0.432 and 0.541 for
G2, G1, and G3, respectively. Furthermore, analysis of molecular variance
recognized 35% variance among and 65% within populations. The present study
showed a high genetic diversity between landraces which can be exploited to
produce new bread wheat cultivars.