scholarly journals Relationship of Phenol Oxidase Activity and Hydroxyl-Radical Generation to Wood Degradation by White-Rot Basidiomycetes

2005 ◽  
Vol 10 (3) ◽  
pp. 85-90 ◽  
Author(s):  
MITSURU YAMAKAWA ◽  
KENGO OZAKL ◽  
TAKU FUJIME ◽  
NAOMI KAKIMOTO ◽  
SHUJI ITAKURA ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Oxidase Activity ◽  
Phenol Oxidase ◽  
White Rot ◽  
Wood Degradation ◽  
Phenol Oxidase Activity ◽  
Radical Generation ◽  
Relationship Of

Phenol Oxidase Activity and One-Electron Oxidation Activity in Wood Degradation by Soft-Rot Deuteromycetes

Holzforschung ◽  
2000 ◽  
Vol 54 (5) ◽  
pp. 463-468 ◽  
Author(s):  
Hiromi Tanaka ◽  
Shuji Itakura ◽  
Akio Enoki
Keyword(s):  
Weight Loss ◽  
Oxidase Activity ◽  
Beech Wood ◽  
Soft Rot ◽  
Phenol Oxidase ◽  
Wood Degradation ◽  
Japanese Beech ◽  
Oxidation Activity ◽  
Phenol Oxidase Activity ◽  
The One

Summary Wood degradation, one-electron oxidation activity as assayed by ethylene production from 2-keto-4-thiomethylbutyric acid, and phenol oxidase activity were measured in cultures of six deuteromycete fungi, with glucose or wood as the carbon source. The four fungi that degraded Japanese beech wood had higher one-electron oxidation activities in wood-containing cultures than in glucose-containing cultures. These four fungi also had measurable phenol oxidase activity in wood-containing cultures, but not in glucose-containing cultures. The two mould fungi that did not significantly degrade wood had no phenol oxidase activity in either wood- or glucose-containing cultures. The one-electron oxidation activity in intact cultures of the soft-rot deuteromycetes was roughly related with the rate of weight loss during wood degradation in those cultures. However, there was no clear relationship between phenol oxidase activity and either one-electron oxidation activity or the rate of wood weight loss, either over time, or in total. Most of the one-electron oxidation activity resulted from phenol oxidase and hydroxyl radical. Most of the phenol oxidase activity resulted from laccase.

Hydroxyl Radical Generation by an Extracellular Low-Molecular-Weight Substance and Phenol Oxidase Activity During Wood Degradation by the White-Rot Basidiomycete Phanerochaete chrysosporium

Holzforschung ◽  
1999 ◽  
Vol 53 (1) ◽  
pp. 21-28 ◽  
Author(s):  
H. Tanaka ◽  
S. Itakura ◽  
A. Enoke
Keyword(s):  
Molecular Weight ◽  
Phanerochaete Chrysosporium ◽  
Hydroxyl Radicals ◽  
Phenol Oxidase ◽  
White Rot ◽  
Low Molecular Weight ◽  
Wood Degradation ◽  
Phenol Oxidase Activity ◽  
Phenol Oxidases ◽  
Weight Substance

SummaryThe mechanism of wood degradation by white-rot fungi is not understood in detail other than it is involving phenol oxidases (which are lignin-degrading enzymes) and cellulases. The possible role of hydroxyl radicals in degradation by the white-rot fungusPhanerochaete chrysosporiumwas studied. The production of a low-molecular-weight substance that is secreted was measured periodically. The substance catalyzes a redox reaction between O2and an electron donor producing hydroxyl radicals in the culture fluid of this fungus with glucose or wood as the carbon source. We examined the possible role(s) of one-electron oxidation activity by phenol oxidases and hydroxyl radicals in wood degradation by this fungus. Assays of phenol oxidase activity showed thatP. chrysosporiumproduced much manganese peroxidase in wood culture, and that the activity peaked early in incubation. The generation of hydroxyl radicals in the redox reaction was related to the rate of wood degradation, but the activity of phenol oxidases was not related. Therefore, hydroxyl radicals are important in wood degradation by this fungus.

Microtopography Alters Hydrology, Phenol Oxidase Activity and Nutrient Availability in Organic Soils of a Coastal Freshwater Forested Wetland

Wetlands ◽  
2018 ◽  
Vol 39 (2) ◽  
pp. 263-273 ◽  
Author(s):  
Kevan J. Minick ◽  
Alexia M. Kelley ◽  
Guofang Miao ◽  
Xuefeng Li ◽  
Asko Noormets ◽  
...  
Keyword(s):  
Nutrient Availability ◽  
Oxidase Activity ◽  
Phenol Oxidase ◽  
Forested Wetland ◽  
Organic Soils ◽  
Phenol Oxidase Activity

scholarly journals Are colorimetric assays appropriate for measuring phenol oxidase activity in peat soils?

2017 ◽  
Vol 105 ◽  
pp. 108-110 ◽  
Author(s):  
Magdalena M. Wiedermann ◽  
Evan S. Kane ◽  
Timothy J. Veverica ◽  
Erik A. Lilleskov
Keyword(s):  
Oxidase Activity ◽  
Phenol Oxidase ◽  
Peat Soils ◽  
Phenol Oxidase Activity ◽  
Colorimetric Assays
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