Can vaccines against canine visceral leishmaniasis interfere with the serological diagnostics recommended by the Brazilian Ministry of Health?

ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations of this dog were negative. Our results showed that in a non-endemic area, even at different evaluation times, dogs vaccinated against CVL with Leishmune® or Leish tec® did not seroconvert in the serological protocol used by the Brazilian Ministry of Health (DPP/EIE).

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Importance of Lutzomyia longipalpis in the dynamics of transmission of canine visceral leishmaniasis in the endemic area of Porteirinha Municipality, Minas Gerais, Brazil

Veterinary Parasitology ◽

10.1016/j.vetpar.2005.05.006 ◽

2005 ◽

Vol 131 (3-4) ◽

pp. 213-220 ◽

Cited By ~ 35

Author(s):

João C. França-Silva ◽

Ricardo A. Barata ◽

Roberto T. da Costa ◽

Érika Michalsky Monteiro ◽

George L. L. Machado-Coelho ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Endemic Area ◽

Minas Gerais ◽

Lutzomyia Longipalpis ◽

Canine Visceral Leishmaniasis

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Occurrence of Lutzomyia longipalpis (Phlebotominae) and canine visceral leishmaniasis in a rural area of Ilha Solteira, SP, Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612014087 ◽

2014 ◽

Vol 23 (4) ◽

pp. 456-462 ◽

Cited By ~ 5

Author(s):

Julio Cesar Pereira Spada ◽

Diogo Tiago da Silva ◽

Kennya Rozy Real Martins ◽

Lílian Aparecida Colebrusco Rodas ◽

Maria Luana Alves ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Rural Area ◽

Antibody Test ◽

Lutzomyia Longipalpis ◽

Climatic Data ◽

Canine Visceral Leishmaniasis ◽

Serological Tests ◽

Relative Air Humidity ◽

Control And Prevention ◽

One Year

This study aimed to investigate the occurrence of Lutzomyia longipalpis and also the canine visceral leishmaniasis (CVL) in a rural area of Ilha Solteira, state of São Paulo. Blood samples were collected from 32 dogs from different rural properties (small farms) and were analyzed by ELISA and the indirect immunofluorescence antibody test (IFAT) in order to diagnose CVL. From these serological tests, 31.25% of the dogs were positive for CVL and these were distributed in 66.7% (8/12) of the rural properties, which were positive for L. longipalpis. CDC (Center for Disease Control and Prevention) light traps were installed in 12 properties (one per property) and insects were caught on three consecutive days per month for one year. L. longipalpis was present on 100% of the rural properties visited, at least once during the twelve-month interval, totaling 64 males and 25 females. The insects were more numerous after the peak of the rain, but the association between prevalence of peridomestic vectors and the climatic data (precipitation, relative air humidity and temperature) and the occurrences of CVL among dogs on each rural property were not statistical significant (p <0.05). However, the occurrence of CVL cases in dogs and the presence of L. longipalpis indicate that more attention is necessairy for the control of this disease in the rural area studied.

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Serodiagnosis and Screening of Canine Visceral Leishmaniasis in an Endemic Area of Corsica: Applicability of a Direct Agglutination Test and Immunoblot Analysis

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.1992.47.772 ◽

1992 ◽

Vol 47 (6) ◽

pp. 772-777 ◽

Cited By ~ 20

Author(s):

Asit B. Neogy ◽

Didier Rzepka ◽

Yiannis Tselentis ◽

Jean C. Lascombe ◽

Ioannis Vouldoukis ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Endemic Area ◽

Immunoblot Analysis ◽

Agglutination Test ◽

Canine Visceral Leishmaniasis ◽

Direct Agglutination Test

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Study of sand fly fauna in an endemic area of American cutaneous leishmaniasis and canine visceral leishmaniasis in the municipality of Espírito Santo do Pinhal, São Paulo, Brazil

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762010000200017 ◽

2010 ◽

Vol 105 (2) ◽

pp. 208-215 ◽

Cited By ~ 16

Author(s):

Fernanda Elisa Colla-Jacques ◽

Cláudio Casanova ◽

Ângelo Pires do Prado

Keyword(s):

Visceral Leishmaniasis ◽

Cutaneous Leishmaniasis ◽

Endemic Area ◽

Sao Paulo ◽

Sand Fly ◽

Canine Visceral Leishmaniasis ◽

São Paulo ◽

Espírito Santo ◽

American Cutaneous Leishmaniasis ◽

Espirito Santo

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Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652010000400005 ◽

2010 ◽

Vol 52 (4) ◽

pp. 193-196 ◽

Cited By ~ 22

Author(s):

Fabiano B. Figueiredo ◽

Maria F. Madeira ◽

Lílian D. Nascimento ◽

Tuanne R. Abrantes ◽

Eliame Mouta-confort ◽

...

Keyword(s):

Visceral Leishmaniasis ◽

Filter Paper ◽

Rio De Janeiro ◽

Serological Test ◽

Fluorescent Antibody ◽

Antibody Test ◽

Canine Visceral Leishmaniasis ◽

Leishmania Chagasi ◽

Blood Samples ◽

One Year

The Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

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