A high degree of cross reactivity between the antigens of Taenia solium and Taenia crassiceps, potentially useful for the diagnosis of human neurocysticercosis (NCC)

1998 ◽  
Vol 58 (6) ◽  
pp. 693-694 ◽  
Author(s):  
H H Garcia ◽  
V C Tsang ◽  
R H Gilman
Keyword(s):  
Taenia Solium ◽  
Cross Reactivity ◽  
Taenia Crassiceps ◽  
High Degree

scholarly journals The diagnostic importance of species specific and cross-reactive components of Taenia solium, Echinococcus granulosus, and Hymenolepis nana

1994 ◽  
Vol 36 (4) ◽  
pp. 327-334 ◽  
Author(s):  
Teresa Montenegro ◽  
Robert H. Gilman ◽  
Rosa Castillo ◽  
Victor Tsang ◽  
Joy Brandt ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Ammonium Sulphate ◽  
Taenia Solium ◽  
Cross Reactivity ◽  
Elisa Assay ◽  
Hymenolepis Nana ◽  
Lentil Lectin ◽  
Species Specific ◽  
High Degree ◽  
Diagnostic Importance

Sera from patients infected with Taenia solium, Hymenolepis nana and Echinococcus granulosus were tested against homologous and heterologous parasite antigens using an ELISA assay, and a high degree of cross-reactivity was verified. To identify polypeptides responsible for this cross reactivity, the Enzyme Linked Immunoelectro Transfer Blot (EITB) was used. Sera from infected patients with T.solium, H.nana, and E.granulosus were assessed against crude, ammonium sulphate precipitated (TSASP), and lentil-lectin purified antigens of T.solium and crude antigens of.H.nana and E.granulosus. Several bands, recognized by sera from patients with T.solium, H.nana, and E.granulosus infections, were common to either two or all three cestodes. Unique reactive bands in H.nana were noted at 49 and 66 K-Da and in E.granulosus at 17-21 K-Da and at 27-32 K-Da. In the crude cysticercosis extract, a specific non glycoprotein band was present at 61-67 K-Da in addiction to specific glycoprotein bands of 50, 42, 24, 21, 18, 14, and 13 K-Da. None of the sera from patients with H.nana or E.granulosus infection cross reacted with these seven glycoprotein bands considered specific for T.solium infection.

scholarly journals ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci

2000 ◽  
Vol 42 (2) ◽  
pp. 71-79 ◽  
Author(s):  
Paulo Sérgio de Arruda PINTO ◽  
Adelaide José VAZ ◽  
Pedro Manuel Leal GERMANO ◽  
Paulo Mutuko NAKAMURA
Keyword(s):  
High Performance ◽  
Taenia Solium ◽  
Elisa Test ◽  
Negative Control ◽  
Cross Reactivity ◽  
Cow Milk ◽  
Tween 20 ◽  
Swine Production ◽  
Serum Samples ◽  
Taenia Crassiceps

In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia solium extracts of scolex (S-Ts) and of larvae (T-Ts). A checkerboard evaluation of antigen, serum and conjugate dilutions, as well as the use of Tween-20 and skim cow milk in wash and blocking solution had a marked effect on improving ELISA performance. All the antigens showed a good performance, but VF-Tcra was the best, with 96.0% and 80.0% sensitivities for cut-offs respectively at 2sd and 3sd, and corresponding specificities of 97.5% and 100.0%. Cross-reactivity was observed only with hydatidosis and ascaridiosis. In view of the high performance observed, the ELISA test should be recommended for the diagnosis of cysticercosis in suspected swine in slaughterhouses and for the screening of cysticercosis in swine production. These results will support integrated measures of cysticercosis control throughout the chain of swine production, effectively contributing to public health.

Cross-reactivity of anti-Taenia crassiceps cysticerci immune antibodies with Taenia solium antigens

2000 ◽  
Vol 89 (4) ◽  
pp. 321-326 ◽  
Author(s):  
N.M. Espı́ndola ◽  
E.N. De Gaspari ◽  
P.M. Nakamura ◽  
A.J. Vaz
Keyword(s):  
Taenia Solium ◽  
Cross Reactivity ◽  
Taenia Crassiceps

scholarly journals Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens

2000 ◽  
Vol 42 (3) ◽  
pp. 175-177 ◽  
Author(s):  
Noeli M. ESPÍNDOLA ◽  
Elizabeth N. DE GASPARI ◽  
Paulo M. NAKAMURA ◽  
Adelaide J. VAZ
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Fusion ◽  
Taenia Solium ◽  
Cross Reactivity ◽  
Taenia Crassiceps ◽  
Igm Antibodies ◽  
Specific Igg

We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra specific IgG-clones, 5 of them also recognizing Tso antigens. Two clones identified the 8-14 and 18kD peptides of VF-Tcra.

scholarly journals Taenia larvae possess distinct acetylcholinesterase profiles with implications for host cholinergic signalling

2020 ◽  
Vol 14 (12) ◽  
pp. e0008966
Author(s):  
Anja de Lange ◽  
Ulrich Fabien Prodjinotho ◽  
Hayley Tomes ◽  
Jana Hagen ◽  
Brittany-Amber Jacobs ◽  
...  
Keyword(s):  
Membrane Surface ◽  
Taenia Solium ◽  
Acetylcholinesterase Activity ◽  
Bioinformatic Analysis ◽  
Functional Protein ◽  
Taenia Crassiceps ◽  
Human Sequence ◽  
Neuronal Signalling ◽  
Whole Cell Patch Clamp ◽  
Acquired Epilepsy

Larvae of the cestodes Taenia solium and Taenia crassiceps infect the central nervous system of humans. Taenia solium larvae in the brain cause neurocysticercosis, the leading cause of adult-acquired epilepsy worldwide. Relatively little is understood about how cestode-derived products modulate host neural and immune signalling. Acetylcholinesterases, a class of enzyme that breaks down acetylcholine, are produced by a host of parasitic worms to aid their survival in the host. Acetylcholine is an important signalling molecule in both the human nervous and immune systems, with powerful modulatory effects on the excitability of cortical networks. Therefore, it is important to establish whether cestode derived acetylcholinesterases may alter host neuronal cholinergic signalling. Here we make use of multiple techniques to profile acetylcholinesterase activity in different extracts of both Taenia crassiceps and Taenia solium larvae. We find that the larvae of both species contain substantial acetylcholinesterase activity. However, acetylcholinesterase activity is lower in Taenia solium as compared to Taenia crassiceps larvae. Further, whilst we observed acetylcholinesterase activity in all fractions of Taenia crassiceps larvae, including on the membrane surface and in the excreted/secreted extracts, we could not identify acetylcholinesterases on the membrane surface or in the excreted/secreted extracts of Taenia solium larvae. Bioinformatic analysis revealed conservation of the functional protein domains in the Taenia solium acetylcholinesterases, when compared to the homologous human sequence. Finally, using whole-cell patch clamp recordings in rat hippocampal brain slice cultures, we demonstrate that Taenia larval derived acetylcholinesterases can break down acetylcholine at a concentration which induces changes in neuronal signalling. Together, these findings highlight the possibility that Taenia larval acetylcholinesterases can interfere with cholinergic signalling in the host, potentially contributing to pathogenesis in neurocysticercosis.

Cu,Zn superoxide dismutase: Cloning and analysis of the Taenia solium gene and Taenia crassiceps cDNA

2012 ◽  
Vol 130 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Ricardo Parra-Unda ◽  
Felipe Vaca-Paniagua ◽  
Lucia Jiménez ◽  
Abraham Landa
Keyword(s):  
Superoxide Dismutase ◽  
Taenia Solium ◽  
Taenia Crassiceps

scholarly journals Characterization of the Duffy-Binding-Like Domain of Plasmodium falciparum Blood-Stage Antigen 332

2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Sandra Nilsson ◽  
Kirsten Moll ◽  
Davide Angeletti ◽  
Letusa Albrecht ◽  
Inari Kursula ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunofluorescence Microscopy ◽  
Homology Model ◽  
Cross Reactivity ◽  
Blood Stage ◽  
Conserved Domain ◽  
Erythrocyte Binding ◽  
High Degree ◽  
Degree Of Similarity

Studies on Pf332, a major Plasmodium falciparum blood-stage antigen, have largely been hampered by the cross-reactive nature of antibodies generated against the molecule due to its high content of repeats, which are present in other malaria antigens. We previously reported the identification of a conserved domain in Pf332 with a high degree of similarity to the Duffy-binding-like (DBL) domains of the erythrocyte-binding-like (EBL) family. We here describe that antibodies towards Pf332-DBL are induced after repeated exposure to P. falciparum and that they are acquired early in life in areas of intense malaria transmission. Furthermore, a homology model of Pf332-DBL was found to be similar to the structure of the EBL-DBLs. Despite their similarities, antibodies towards Pf332-DBL did not display any cross-reactivity with EBL-proteins as demonstrated by immunofluorescence microscopy, Western blotting, and peptide microarray. Thus the DBL domain is an attractive region to use in further studies on the giant Pf332 molecule.

scholarly journals Cephalosporins’ Cross-Reactivity and the High Degree of Required Knowledge. Case Report and Review of the Literature

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 209
Author(s):  
Stefano D’Errico ◽  
Paola Frati ◽  
Martina Zanon ◽  
Eleonora Valentinuz ◽  
Federico Manetti ◽  
...  
Keyword(s):  
Present Report ◽  
Specific Ige ◽  
Chain Structure ◽  
Cross Reactivity ◽  
Side Chain ◽  
Mast Cell Tryptase ◽  
Immunohistochemical Examination ◽  
Femoral Blood ◽  
The Subject ◽  
High Degree

Antibiotic cross-reactivity represents a phenomenon of considerable interest as well as antibiotic resistance. Immediate reactions to cephalosporins are reported in the literature with a prevalence of only 1–3% of the population, while anaphylactic reactions are rarely described (approximately 0.0001–0.1%) as well as fatalities. Allergic reaction to cephalosporins may occur because of sensitization to unique cephalosporin haptens or to determinants shared with penicillins. Cross-reactivity between cephalosporins represents, in fact, a well-known threatening event involving cephalosporins with similar or identical R1- or R2-side chains. The present report describes the case of a 79-year-old man who suddenly died after intramuscular administration of ceftriaxone. Serum dosage of mast cell tryptase from a femoral blood sample at 3 and 24 h detected values of 87.7μg/L and 93.5μg/L, respectively (cut-off value 44.3 μg/L); the serum-specific IgE for penicillins, amoxicillin, cephaclor and also for the most common allergens were also determined. A complete post-mortem examination was performed, including gross, histological and immunohistochemical examination, with an anti-tryptase antibody. The cause of death was identified as anaphylactic shock: past administrations of cefepime sensitized the subject to cephalosporins and a fatal cross-reactivity of ceftriaxone with cefepime occurred due to the identical seven-position side chain structure in both molecules. The reported case offers food for thought regarding the study of cross-reactivity and the need to clarify the predictability and preventability of the phenomenon in fatal events.

scholarly journals Cross reactivity of neutralizing antibodies to the encephalitic California Serogroup orthobunyaviruses varies by virus and genetic relatedness

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alyssa B. Evans ◽  
Karin E. Peterson
Keyword(s):  
Gold Standard ◽  
Neutralizing Antibodies ◽  
Viral Encephalitis ◽  
Genetic Relatedness ◽  
Cross Reactivity ◽  
Etiologic Agent ◽  
Snowshoe Hare ◽  
Neuroinvasive Disease ◽  
California Serogroup ◽  
High Degree

AbstractThe California Serogroup (CSG) of Orthobunyaviruses comprises several viruses capable of causing neuroinvasive disease in humans, including La Crosse (LACV), Snowshoe Hare (SSHV), Tahyna (TAHV), Jamestown Canyon (JCV), and Inkoo (INKV) viruses. Diagnosis of specific CSG viruses is complicated by the high degree of antibody cross-reactivity between them, with laboratory standards requiring a fourfold higher titer of neutralizating antibody (NAb) activity to positively identify the etiologic virus. To help elucidate NAb relationships between neuroinvasive CSG viruses, we directly compared the cross-reactivity of NAb between LACV, SSHV, TAHV, JCV, and INKV. Mice were inoculated with individual viruses and the NAb activity of plasma samples was compared by plaque reduction neutralization tests against all five viruses. Overall, the results from these studies show that the CSG viruses induced high levels of NAb against the inoculum virus, and differing amounts of cross-reactive NAb against heterologous viruses. LACV, SSHV, and INKV elicited the highest amount of cross-reactive NAb. Interestingly, a fourfold difference in NAb titer between the inoculum virus and the other CSG viruses was not always observed. Thus, NAb titers, which are the gold-standard for diagnosing the etiologic agent for viral encephalitis, may not clearly differentiate between different CSG viruses.

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