scholarly journals Laboratory and Field Evaluation of the Crystal VC-O1 Cholera Rapid Diagnostic Test

2021 ◽  
Author(s):  
Amanda K. Debes ◽  
Kelsey N. Murt ◽  
Ethyl Waswa ◽  
Gerald Githinji ◽  
Mama Umuro ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
High Performance ◽  
Performance Metrics ◽  
Diarrheal Disease ◽  
Low Cost ◽  
Field Evaluation ◽  
Outbreak Detection ◽  
Case Definitions ◽  
Dipstick Test

Cholera is a severe acute, highly transmissible diarrheal disease which affects many low- and middle-income countries. Outbreaks of cholera are confirmed using microbiological culture, and additional cases during the outbreak are generally identified based on clinical case definitions, rather than laboratory confirmation. Many low-resource areas where cholera occurs lack the capacity to perform culture in an expeditious manner. A simple, reliable, and low-cost rapid diagnostic test (RDT) would improve identification of cases allowing rapid response to outbreaks. Several commercial RDTs are available for cholera testing with two lines to detect either serotypes O1 and O139; however, issues with sensitivity and specificity have not been optimal with these bivalent tests. Here, we report an evaluation of a new commercially available cholera dipstick test which detects only serotype O1. In both laboratory and field studies in Kenya, we demonstrate high sensitivity (97.5%), specificity (100%), and positive predictive value (100%) of this new RDT targeting only serogroup O1. This is the first field evaluation for the new Crystal VC-O1 RDT; however, with these high-performance metrics, this RDT could significantly improve cholera outbreak detection and improve surveillance for better understanding of cholera disease burden.

scholarly journals Field evaluation of a rapid diagnostic test to detect antibodies in human toxocariasis

Acta Tropica ◽  
2015 ◽  
Vol 148 ◽  
pp. 32-37 ◽  
Author(s):  
P.K.C. Lim ◽  
H. Yamasaki ◽  
J.W. Mak ◽  
S.F. Wong ◽  
C.W. Chong ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Field Evaluation

scholarly journals Field evaluation of a Pan-Lassa rapid diagnostic test during the 2018 Nigerian Lassa fever outbreak

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Matthew L. Boisen ◽  
Eghosa Uyigue ◽  
John Aiyepada ◽  
Katherine J. Siddle ◽  
Lisa Oestereich ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Field Evaluation ◽  
Lassa Fever

scholarly journals Field Evaluation of Cholkit Rapid Diagnostic Test for Vibrio Cholerae O1 During a Cholera Outbreak in Malawi, 2018

2020 ◽  
Vol 7 (11) ◽  
Author(s):  
Innocent Chibwe ◽  
Watipaso Kasambara ◽  
Mathews Kagoli ◽  
Harry Milala ◽  
Charity Gondwe ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Diagnostic Tests ◽  
Field Evaluation ◽  
Rapid Diagnostic Tests ◽  
Cholera Outbreak ◽  
Vibrio Cholerae O1

Abstract Rapid diagnostic tests (RDTs) for cholera are an important emerging tool for surveillance, yet the currently available tests have several limitations. We assess the performance of a new RDT, Cholkit, during a cholera outbreak in Malawi compared with culture and find a sensitivity of 93.0% (95% CI, 83.0%–98.1%) and a specificity of 95.7% (95% CI, 78.1%–100.0%).

scholarly journals Field evaluation of a rapid diagnostic test (ParascreenTM) for malaria diagnosis in the Peruvian Amazon

2010 ◽  
Vol 9 (1) ◽  
pp. 154 ◽  
Author(s):  
Jorge Bendezu ◽  
Angel Rosas ◽  
Tanilu Grande ◽  
Hugo Rodriguez ◽  
Alejandro Llanos-Cuentas ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Malaria Diagnosis ◽  
Field Evaluation ◽  
Peruvian Amazon

scholarly journals Feasibility of a Combined Mobile-Health Electrocardiographic and Rapid Diagnostic Test Screening for Chagas-Related Cardiac Alterations

2021 ◽  
Vol 9 (9) ◽  
pp. 1889
Author(s):  
Michele Spinicci ◽  
Carlo Fumagalli ◽  
Niccolò Maurizi ◽  
Enrico Guglielmi ◽  
Mimmo Roselli ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Mobile Health ◽  
Low Cost ◽  
Diagnostic System ◽  
Chagas Cardiomyopathy ◽  
Cardiology Clinic ◽  
Low Cost Strategy ◽  
User Friendly ◽  
Ecg Abnormalities

Background: Chronic Chagas cardiomyopathy (CChC) is the most common cause of death related to Chagas disease (CD). The aim of this study was to assess the feasibility of a combined rapid diagnostic test (RDT) and electrocardiographic (ECG) screening in a remote rural village of the Bolivian Chaco, with a high prevalence of CChC. Methods: Consecutive healthy volunteers > 15 years were enrolled in the community of Palmarito (municipality of Gutierrez, Santa Cruz Department, Bolivia) in February 2019. All patients performed an RDT with Chagas Stat-Pak® (CSP, Chembio Diagnostic System, Medford, NY, USA) and an ECG by D-Heart® technology, a low-cost, user-friendly smartphone-based 8-lead Bluetooth ECG. RDTs were read locally while ECGs were sent to a cardiology clinic which transmitted reports within 24 h from recording. Results: Among 140 people (54 men, median age 38(interquartile range 23–54) years), 98 (70%) were positive for Trypanosoma cruzi infection, with a linear, age-dependent, increasing trend (p < 0.001). Twenty-five (18%) individuals showed ECG abnormalities compatible with CD. Prevalence of ECG abnormalities was higher in infected individuals and was associated with higher systolic blood pressure and smoking. Following screening, 22 (16%) individuals underwent clinical evaluation and chest X-ray and two were referred for further evaluation. At multivariate analysis, positive CSP results (OR = 4.75, 95%CI 1.08–20.96, p = 0.039) and smoking (OR = 4.20, 95%CI 1.18–14.92, p = 0.027) were independent predictors of ECG abnormalities. Overall cost for screening implementation was <10 $. Conclusions: Combined mobile-Health and RDTs was a reliable and effective low-cost strategy to identify patients at high risk of disease needing cardiologic assessment suggesting potential future applications.

scholarly journals A Novel Automatic Rapid Diagnostic Test Reader Platform

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Haydar Ozkan ◽  
Osman Semih Kayhan
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Personal Information ◽  
Low Cost ◽  
Region Of Interest ◽  
Laptop Computer ◽  
Lateral Flow Strip ◽  
Contagious Diseases ◽  
H Pylori ◽  
Information Image

A novel automatic Rapid Diagnostic Test (RDT) reader platform is designed to analyze and diagnose target disease by using existing consumer cameras of a laptop-computer or a tablet. The RDT reader is useable with numerous lateral immunochromatographic assays and similar biomedical tests. The system has two different components, which are 3D-printed, low-cost, tiny, and compact stand and a decision program named RDT-AutoReader 2.0. The program takes the image of RDT, crops the region of interest (ROI), and extracts the features from the control end test lines to classify the results as invalid, positive, or negative. All related patient’s personal information, image of ROI, and the e-report are digitally saved and transferred to the related clinician. Condition of the patient and the progress of the disease can be monitored by using the saved data. The reader platform has been tested by taking image from used cassette RDTs of rotavirus (RtV)/adenovirus (AdV) and lateral flow strip RDTs ofHelicobacter pylori(H. pylori) before discarding them. The created RDT reader can also supply real-time statistics of various illnesses by using databases and Internet. This can help to inhibit propagation of contagious diseases and to increase readiness against epidemic diseases worldwide.

scholarly journals Field evaluation of a locally produced rapid diagnostic test for early detection of cholera in Bangladesh

2019 ◽  
Vol 13 (1) ◽  
pp. e0007124 ◽  
Author(s):  
Md. Taufiqul Islam ◽  
Ashraful Islam Khan ◽  
Md. Abu Sayeed ◽  
Jakia Amin ◽  
Kamrul Islam ◽  
...  
Keyword(s):  
Early Detection ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Field Evaluation

scholarly journals Gold-standard diagnostics are tarnished by lytic bacteriophage

2020 ◽  
Author(s):  
E.J. Nelson ◽  
J.A. Grembi ◽  
D.L. Chao ◽  
J.R. Andrews ◽  
L. Alexandrova ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Gold Standard ◽  
Diagnostic Performance ◽  
Diarrheal Disease ◽  
Current Approach ◽  
Lytic Bacteriophage ◽  
Antibiotic Exposure ◽  
First Line ◽  
Negative Effect

ABSTRACTBackgroundA fundamental clinical and scientific concern is how lytic bacteriophage, as well as antibiotics, impact diagnostic positivity.MethodsCholera was chosen as a model disease to investigate this important question. Patients with diarrheal disease were enrolled at two remote hospitals in Bangladesh. Diagnostic performance was assessed as a function of lytic bacteriophage detection, as well as exposure to the first-line antibiotic azithromycin detected by mass spectrometry.ResultsAmong diarrheal samples positive by nanoliter quantitative PCR for Vibrio cholerae (n=78/849), the odds that a rapid diagnostic test (RDT) or qPCR was positive was reduced by 89% (OR 0.108; 95%CI 0.002-0.872) and 87% (OR 0.130; 95%CI 0.022-0.649) when lytic bacteriophage were detected, respectively. The odds that a rapid diagnostic test (RDT) or qPCR was positive was reduced by more than 99% (OR 0.00; 95% CI: 0.00-0.28) and 89% (OR 0.11; 95% CI: 0.03-0.44) when azithromycin was detected, respectively.ConclusionsEstimations of cholera burden may improve by accommodating for the negative effect of antimicrobial exposure on diagnostic positivity. Furthermore, the findings herein challenge our current approach to interpreting and developing bacterial diagnostics given variable rates of lytic bacteriophage and antibiotic exposure.

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