MOLECULAR EVIDENCE OF BABESIA EQUI TRANSMISSION IN HAEMAPHYSALIS LONGICORNIS

2007 ◽  
Vol 76 (4) ◽  
pp. 694-697 ◽  
Author(s):  
HIROMI IKADAI ◽  
AYA MATSUU ◽  
MIZUKI SASAKI ◽  
HIDEKAZU ISHIDA ◽  
KOZO FUJISAKI ◽  
...  
2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Lijun Shao ◽  
Ruiheng Chang ◽  
Lin Liu ◽  
Yongjin Wang ◽  
Yun Gao ◽  
...  

AbstractDabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, a total of 170 engorged ticks and 22 sheep serum samples were collected from Taian and Yantai city, Shandong Province to investigate the presence of DTV. The results of qRT-PCR revealed the positive rate of 13.6% (3/22) in sheep serum and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV might be relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not causing disease in animals, DTV deserves further investigation.


2021 ◽  
Author(s):  
Lijun Shao ◽  
Ruiheng Chang ◽  
Lin Liu ◽  
Yongjin Wang ◽  
Yun Gao ◽  
...  

Abstract Dabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, the presence of DTV in ticks and sheep samples was investigated. A total of 170 engorged ticks were collected from May to September 2019 from Taian and Yantai city, Shandong Province and were pooled individually to screening for DTV. The results of of qRT-PCR revealed the positive rate of 13.6% (3/22) in serum of sheep and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV was relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not could cause disease in sheep and other animals, DTV deserves further investigation.


Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1143
Author(s):  
Anan Wang ◽  
Zheng Pang ◽  
Lin Liu ◽  
Qianwen Ma ◽  
Yize Han ◽  
...  

Dabieshan tick virus (DTV) is a novel tick-borne virus with the potential to infect both animals and humans. It has been confirmed that DTV is widely distributed in Shandong and Zhejiang Provinces. In this study, a total of 389 ticks were sampled from Honghe city of Yunnan Province and Bijie city of Guizhou Province, and then divided into 148 pools according to the location and species. QRT-PCR and nested PCR were performed to confirm the presence of DTV. The results showed a minimum infection rate of 2.43% (5/206) in Yunnan Province and 3.28% (6/183) in Guizhou Province, respectively. Interestingly, DTV was identified in Rhipicephalusmicroplus for the first time besides Haemaphysalis longicornis. Phylogenetic analysis showed that DTV from Yunnan and Guizhou Provinces shared over 94% identity with isolates derived from Hubei and Shandong Provinces, and DTV was relatively conservative in evolutionary dynamics. These findings provide molecular evidence of Dabieshan tick virus in different species of ticks from unrecognized endemic regions and suggest that DTV may be widely prevalent in southwestern China.


2007 ◽  
Vol 75 (7) ◽  
pp. 3633-3640 ◽  
Author(s):  
Naotoshi Tsuji ◽  
Badgar Battsetseg ◽  
Damdinsuren Boldbaatar ◽  
Takeharu Miyoshi ◽  
Xuenan Xuan ◽  
...  

ABSTRACT Antimicrobial peptides are major components of host innate immunity, a well-conserved, evolutionarily ancient defensive mechanism. Infectious disease-bearing vector ticks are thought to possess specific defense molecules against the transmitted pathogens that have been acquired during their evolution. We found in the tick Haemaphysalis longicornis a novel parasiticidal peptide named longicin that may have evolved from a common ancestral peptide resembling spider and scorpion toxins. H. longicornis is the primary vector for Babesia sp. parasites in Japan. Longicin also displayed bactericidal and fungicidal properties that resemble those of defensin homologues from invertebrates and vertebrates. Longicin showed a remarkable ability to inhibit the proliferation of merozoites, an erythrocyte blood stage of equine Babesia equi, by killing the parasites. Longicin was localized at the surface of the Babesia sp. parasites, as demonstrated by confocal microscopic analysis. In an in vivo experiment, longicin induced significant reduction of parasitemia in animals infected with the zoonotic and murine B. microti. Moreover, RNA interference data demonstrated that endogenous longicin is able to directly kill the canine B. gibsoni, thus indicating that it may play a role in regulating the vectorial capacity in the vector tick H. longicornis. Theoretically, longicin may serve as a model for the development of chemotherapeutic compounds against tick-borne disease organisms.


2001 ◽  
Vol 120 (5) ◽  
pp. A171-A171
Author(s):  
M STONER ◽  
D YAGER ◽  
J KELLUM
Keyword(s):  

2019 ◽  
pp. 23-36
Author(s):  
Mario. R. Cabrera

Formerly Cnemidophorus was thought to be the most speciose genus of Teiidae. This genus comprised four morphological groups that were later defined as four different genera, Ameivula, Aurivela, Cnemidophorus and Contomastix. The last appears as paraphyletic in a recent phylogenetic reconstruction based on morphology, but monophyletic in a reconstruction using molecular characters. Six species are allocated to Contomastix. One of them, C. lacertoides, having an extensive and disjunct geographic distribution in Argentina, Uruguay and Brazil. Preliminary analyses revealed morphological differences among its populations, suggesting that it is actually a complex of species. Here, we describe a new species corresponding to the Argentinian populations hitherto regarded as C. lacertoides, by integrating morphological and molecular evidence. Furthermore, we demonstrate that the presence of notched proximal margin of the tongue is a character that defines the genus Contomastix.


2014 ◽  
Author(s):  
Ursula Foger-Samwald ◽  
Alaghebandan Afarin ◽  
Janina Patsch ◽  
Doris Schamall ◽  
Sylvia Salem ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document