Babesia sp. BQ1 (Lintan): Molecular evidence of experimental transmission to sheep by Haemaphysalis qinghaiensis and Haemaphysalis longicornis

AbstractDabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, a total of 170 engorged ticks and 22 sheep serum samples were collected from Taian and Yantai city, Shandong Province to investigate the presence of DTV. The results of qRT-PCR revealed the positive rate of 13.6% (3/22) in sheep serum and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV might be relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not causing disease in animals, DTV deserves further investigation.

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Experimental Transmission of Babesia ovata oshimensis n. var. of Cattle in Japan by Haemaphysalis longicornis.

Journal of Veterinary Medical Science ◽

10.1292/jvms.58.11_1153 ◽

1996 ◽

Vol 58 (11) ◽

pp. 1153-1155 ◽

Cited By ~ 12

Author(s):

Masato OHTA ◽

Shin-ichiro KAWAZU ◽

Yutaka TERADA ◽

Tsugihiko KAMIO ◽

Masayoshi TSUJI ◽

...

Keyword(s):

Haemaphysalis Longicornis ◽

Experimental Transmission ◽

Babesia Ovata

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Molecular evidence of Babesia caballi (Nuttall and Strickland, 1910) parasite transmission from experimentally-infected SCID mice to the ixodid tick, Haemaphysalis longicornis (Neuman, 1901)

Veterinary Parasitology ◽

10.1016/s0304-4017(01)00531-3 ◽

2001 ◽

Vol 102 (3) ◽

pp. 185-191 ◽

Cited By ~ 7

Author(s):

José Luis Rodrı́guez Bautista ◽

Hiromi Ikadai ◽

Myungjo You ◽

Badgar Battsetseg ◽

Ikuo Igarashi ◽

...

Keyword(s):

Ixodid Tick ◽

Scid Mice ◽

Molecular Evidence ◽

Haemaphysalis Longicornis ◽

Parasite Transmission ◽

Babesia Caballi

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Detection and Phylogenetic Analysis of a Novel Tick-borne Virus in Haemaphysalis Longicornis Ticks and Sheep From Shandong, China

10.21203/rs.3.rs-680468/v1 ◽

2021 ◽

Author(s):

Lijun Shao ◽

Ruiheng Chang ◽

Lin Liu ◽

Yongjin Wang ◽

Yun Gao ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Evolutionary Relationship ◽

Human Remains ◽

Domestic Animals ◽

Molecular Evidence ◽

Haemaphysalis Longicornis ◽

Pathogenic Potential ◽

Qrt Pcr ◽

Positive Rate ◽

Yantai City

Abstract Dabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, the presence of DTV in ticks and sheep samples was investigated. A total of 170 engorged ticks were collected from May to September 2019 from Taian and Yantai city, Shandong Province and were pooled individually to screening for DTV. The results of of qRT-PCR revealed the positive rate of 13.6% (3/22) in serum of sheep and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV was relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not could cause disease in sheep and other animals, DTV deserves further investigation.

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Experimental transmission of an unnamed bovine Babesia by Hyalomma spp., Haemaphysalis longicornis and Boophilus microplus

Veterinary Parasitology ◽

10.1016/s0304-4017(03)00262-0 ◽

2003 ◽

Vol 116 (2) ◽

pp. 115-124 ◽

Cited By ~ 17

Author(s):

Jianxun Luo ◽

Fuyan Chen ◽

Wenshun Lu ◽

Guiquan Guan ◽

Miling Ma ◽

...

Keyword(s):

Boophilus Microplus ◽

Haemaphysalis Longicornis ◽

Experimental Transmission

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Detection and Phylogenetic Analysis of a Novel Tick-Borne Virus in Yunnan and Guizhou Provinces, Southwestern China

Pathogens ◽

10.3390/pathogens10091143 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1143

Author(s):

Anan Wang ◽

Zheng Pang ◽

Lin Liu ◽

Qianwen Ma ◽

Yize Han ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Yunnan Province ◽

Evolutionary Dynamics ◽

Guizhou Province ◽

Southwestern China ◽

Molecular Evidence ◽

Haemaphysalis Longicornis ◽

Qrt Pcr ◽

First Time ◽

Minimum Infection Rate

Dabieshan tick virus (DTV) is a novel tick-borne virus with the potential to infect both animals and humans. It has been confirmed that DTV is widely distributed in Shandong and Zhejiang Provinces. In this study, a total of 389 ticks were sampled from Honghe city of Yunnan Province and Bijie city of Guizhou Province, and then divided into 148 pools according to the location and species. QRT-PCR and nested PCR were performed to confirm the presence of DTV. The results showed a minimum infection rate of 2.43% (5/206) in Yunnan Province and 3.28% (6/183) in Guizhou Province, respectively. Interestingly, DTV was identified in Rhipicephalusmicroplus for the first time besides Haemaphysalis longicornis. Phylogenetic analysis showed that DTV from Yunnan and Guizhou Provinces shared over 94% identity with isolates derived from Hubei and Shandong Provinces, and DTV was relatively conservative in evolutionary dynamics. These findings provide molecular evidence of Dabieshan tick virus in different species of ticks from unrecognized endemic regions and suggest that DTV may be widely prevalent in southwestern China.

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