Molecular evidence of Babesia caballi (Nuttall and Strickland, 1910) parasite transmission from experimentally-infected SCID mice to the ixodid tick, Haemaphysalis longicornis (Neuman, 1901)

A cDNA encoding the vitellogenin receptor of the ixodid tick, Haemaphysalis longicornis Neumann (HlVgR) was cloned and characterized. The full-length cDNA is 5631 bp, including an intact ORF encoding an expected protein with 1782 amino acids. The deduced amino acid sequence of the HlVgR cDNA revealed two ligand-binding domains with four class A cysteine-rich repeats in the first domain and eight in the second domain similar to those of insect VgRs. The immunoblot analysis detected ~197 kDa protein in both tick ovary and egg. The developmental expression profile demonstrated that HlVgR mRNA exists throughout the ovarian development, and the transcriptional level is especially high in the previtellogenic period. Immuno electron microscopy analysis demonstrated that the localization of HlVgR is detected on the external surface of oocyte plasma membrane. RNAi showed that eggs of HlVgR dsRNA-injected adult ticks had not developed into fully mature oocytes and laid abnormal eggs. The Babesia parasite DNA was not detected in the eggs of HlVgR dsRNA-injected tick that fed on Babesia gibsoni infected dog, whereas it was detected in the eggs of PBS-injected ticks and noninjected ticks. Expression of HlVgR was increased by the vitellogenic hormone 20-hydroxyecdysone. These results indicate that HlVgR, which is produced by the developing oocytes, is essential for Vg uptake, egg development in the H. longicornis tick, and transovarial transmission of Babesia parasites.

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Detection and phylogenetic analysis of a novel tick-borne virus in Haemaphysalis longicornis ticks and sheep from Shandong, China

Virology Journal ◽

10.1186/s12985-021-01704-y ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Lijun Shao ◽

Ruiheng Chang ◽

Lin Liu ◽

Yongjin Wang ◽

Yun Gao ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Evolutionary Relationship ◽

Domestic Animals ◽

Molecular Evidence ◽

Haemaphysalis Longicornis ◽

Serum Samples ◽

Pathogenic Potential ◽

Qrt Pcr ◽

Positive Rate ◽

Yantai City

AbstractDabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, a total of 170 engorged ticks and 22 sheep serum samples were collected from Taian and Yantai city, Shandong Province to investigate the presence of DTV. The results of qRT-PCR revealed the positive rate of 13.6% (3/22) in sheep serum and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV might be relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not causing disease in animals, DTV deserves further investigation.

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A serine proteinase inhibitor (serpin) from ixodid tick Haemaphysalis longicornis; cloning and preliminary assessment of its suitability as a candidate for a tick vaccine

Vaccine ◽

10.1016/s0264-410x(03)00167-1 ◽

2003 ◽

Vol 21 (21-22) ◽

pp. 2844-2851 ◽

Cited By ~ 60

Author(s):

Maiko Sugino ◽

Saiki Imamura ◽

Albert Mulenga ◽

Mie Nakajima ◽

Akiko Tsuda ◽

...

Keyword(s):

Proteinase Inhibitor ◽

Serine Proteinase ◽

Ixodid Tick ◽

Haemaphysalis Longicornis ◽

Serine Proteinase Inhibitor ◽

Preliminary Assessment ◽

Tick Vaccine

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The Intracellular and Extracellular Hemoglobin Degradations in The Ixodid Tick, Haemaphysalis longicornis(Special Lecture,Proceedings of the 52nd Annual Meeting of Northern Region)

Medical Entomology and Zoology ◽

10.7601/mez.57.151_1 ◽

2006 ◽

Vol 57 (2) ◽

pp. 151

Author(s):

K. Fujisaki

Keyword(s):

Annual Meeting ◽

Ixodid Tick ◽

Northern Region ◽

Haemaphysalis Longicornis ◽

Special Lecture

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HlCPL-A, a cathepsin L-like cysteine protease from the ixodid tick Haemaphysalis longicornis, modulated midgut proteolytic enzymes and their inhibitors during blood meal digestion

Infection Genetics and Evolution ◽

10.1016/j.meegid.2013.01.018 ◽

2013 ◽

Vol 16 ◽

pp. 206-211 ◽

Cited By ~ 5

Author(s):

Kayoko Yamaji ◽

Takeharu Miyoshi ◽

Takeshi Hatta ◽

Makoto Matsubayashi ◽

M. Abdul Alim ◽

...

Keyword(s):

Cysteine Protease ◽

Blood Meal ◽

Proteolytic Enzymes ◽

Cathepsin L ◽

Ixodid Tick ◽

Haemaphysalis Longicornis ◽

Blood Meal Digestion

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Molecular and microscopic detection of Babesia caballi and Theileria equi among working horses and donkeys in Cairo and Giza provinces of Egypt

10.21203/rs.3.rs-757240/v1 ◽

2021 ◽

Author(s):

Ahmed M. Soliman ◽

Nagwa M. Elhawary ◽

Nashwa M. Helmy ◽

Sahar M. Gadelhaq

Keyword(s):

Ixodid Tick ◽

Economic Losses ◽

Age Group ◽

Health Issues ◽

Conventional Pcr ◽

Theileria Equi ◽

Babesia Caballi ◽

Tick Borne Disease ◽

The Difference ◽

Age And Sex

Abstract Equine piroplasmosis (EP) is an ixodid tick-borne disease caused by Theileria equi and/or Babesia caballi that can lead to severe health issues and economic losses among equine population. This study aimed to determine the prevalence of T. equi and B. caballi among Egyptian equines based on microscopy and conventional PCR. Also, to determine the effect of season, age, and sex of on their prevalence and determining the difference in sensitivity between microscopy and conventional PCR in the diagnosis of EP. This study was carried out on 432 blood samples randomly collected from 146 horses and 286 donkeys during a period from April 2016 to March 2018. Microscopic examination revealed that among horses, 13 (8.9%) and 4 (2.7%) were infected by T. equi and B. caballi respectively. While among donkeys, 22 (7.7%), 16 (5.6%) respectively. While mixed infections were detected in 4 (1.4%) donkeys. There was a statistically nonsignificant relation between prevalence of infection and season and sex of equines but the highest prevalence was recorded in age group less than 5 years old. By conventional PCR, among 64 horses, 15 (23.4%) and 8 (12.5%) were infected by T. equi and B. caballi, respectively. While among 76 donkeys, 36 (47.4%), 16 (21.1%), and 5 (6.6%) were infected by T. equi, B. caballi, and mixed infection, respectively. Our finding proved the existence of T. equi and B. caballi among equines.

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