Detection and Phylogenetic Analysis of a Novel Tick-Borne Virus in Yunnan and Guizhou Provinces, Southwestern China

Dabieshan tick virus (DTV) is a novel tick-borne virus with the potential to infect both animals and humans. It has been confirmed that DTV is widely distributed in Shandong and Zhejiang Provinces. In this study, a total of 389 ticks were sampled from Honghe city of Yunnan Province and Bijie city of Guizhou Province, and then divided into 148 pools according to the location and species. QRT-PCR and nested PCR were performed to confirm the presence of DTV. The results showed a minimum infection rate of 2.43% (5/206) in Yunnan Province and 3.28% (6/183) in Guizhou Province, respectively. Interestingly, DTV was identified in Rhipicephalusmicroplus for the first time besides Haemaphysalis longicornis. Phylogenetic analysis showed that DTV from Yunnan and Guizhou Provinces shared over 94% identity with isolates derived from Hubei and Shandong Provinces, and DTV was relatively conservative in evolutionary dynamics. These findings provide molecular evidence of Dabieshan tick virus in different species of ticks from unrecognized endemic regions and suggest that DTV may be widely prevalent in southwestern China.

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Detection and phylogenetic analysis of a novel tick-borne virus in Haemaphysalis longicornis ticks and sheep from Shandong, China

Virology Journal ◽

10.1186/s12985-021-01704-y ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Lijun Shao ◽

Ruiheng Chang ◽

Lin Liu ◽

Yongjin Wang ◽

Yun Gao ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Evolutionary Relationship ◽

Domestic Animals ◽

Molecular Evidence ◽

Haemaphysalis Longicornis ◽

Serum Samples ◽

Pathogenic Potential ◽

Qrt Pcr ◽

Positive Rate ◽

Yantai City

AbstractDabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, a total of 170 engorged ticks and 22 sheep serum samples were collected from Taian and Yantai city, Shandong Province to investigate the presence of DTV. The results of qRT-PCR revealed the positive rate of 13.6% (3/22) in sheep serum and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV might be relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not causing disease in animals, DTV deserves further investigation.

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Detection and Phylogenetic Analysis of a Novel Tick-borne Virus in Haemaphysalis Longicornis Ticks and Sheep From Shandong, China

10.21203/rs.3.rs-680468/v1 ◽

2021 ◽

Author(s):

Lijun Shao ◽

Ruiheng Chang ◽

Lin Liu ◽

Yongjin Wang ◽

Yun Gao ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Evolutionary Relationship ◽

Human Remains ◽

Domestic Animals ◽

Molecular Evidence ◽

Haemaphysalis Longicornis ◽

Pathogenic Potential ◽

Qrt Pcr ◽

Positive Rate ◽

Yantai City

Abstract Dabieshan tick virus (DTV) was first identified in Haemaphysalis longicornis from Hubei Province, China in 2015. However, its pathogenic potential to animals and human remains to be further explored. In this study, the presence of DTV in ticks and sheep samples was investigated. A total of 170 engorged ticks were collected from May to September 2019 from Taian and Yantai city, Shandong Province and were pooled individually to screening for DTV. The results of of qRT-PCR revealed the positive rate of 13.6% (3/22) in serum of sheep and 8.2% (14/170) in attached ticks, respectively. Phylogenetic analysis demonstrated a close evolutionary relationship among those DTV isolates from animal and ticks, and DTV was relatively conservative in evolution. These findings are the first to demonstrate molecular evidence of DTV in domestic animals. Nonetheless, whether or not could cause disease in sheep and other animals, DTV deserves further investigation.

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Rhomboidia wuliangshanensis gen. & sp. nov. from southwestern China

Mycotaxon ◽

10.5248/134.649 ◽

2020 ◽

Vol 134 (4) ◽

pp. 649-662

Author(s):

Tai-Min Xu ◽

Xiang-Fu Liu ◽

Yu-Hui Chen ◽

Chang-Lin Zhao

Keyword(s):

Yunnan Province ◽

Southwest China ◽

Phylogenetic Analyses ◽

White Rot ◽

Southwestern China ◽

Molecular Evidence ◽

Hyphal System ◽

Monophyletic Lineage ◽

Nuclear Rna ◽

Fungal Genus

A new, white-rot, poroid, wood-inhabiting fungal genus, Rhomboidia, typified by R. wuliangshanensis, is proposed based on morphological and molecular evidence. Collected from subtropical Yunnan Province in southwest China, Rhomboidia is characterized by annual, stipitate basidiomes with rhomboid pileus, a monomitic hyphal system with thick-walled generative hyphae bearing clamp connections, and broadly ellipsoid basidiospores with thin, hyaline, smooth walls. Phylogenetic analyses of ITS and LSU nuclear RNA gene regions showed that Rhomboidia is in Steccherinaceae and formed as distinct, monophyletic lineage within a subclade that includes Nigroporus, Trullella, and Flabellophora .

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A new species of Skeletocutis (Polyporales, Basidiomycota) from Yunnan of China

Phytotaxa ◽

10.11646/phytotaxa.270.4.3 ◽

2016 ◽

Vol 270 (4) ◽

pp. 267 ◽

Cited By ~ 1

Author(s):

LU-SEN BIAN ◽

CHANG-LIN ZHAO ◽

FANG WU

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Ribosomal Rna ◽

Internal Transcribed Spacer ◽

Yunnan Province ◽

Large Subunit ◽

Morphological Characteristics ◽

Molecular Evidence ◽

Its Regions ◽

A New Species

A new species of Polyporales, named as Skeletocutis yunnanensis, was collected on angiosperm wood in northern Yunnan Province, southwestern China. It is described based on morphological characteristics and molecular evidence. The species belongs to the Skeletocutis subincarnata complex, but differs morphologically from all known species of the genus by white, cream to buff pores surface, angular pores mostly 5–6 per mm with entire mouths, a dimitic hyphal structure both in trama and subiculum, generative hyphae in whole basidiocarps covered by fine crystals, skeletal hyphae unchanged in KOH, not agglutinated, allantoid basidiospores measured as 3.5–4.5 × 1.0–1.2 µm, and growth on angiosperm wood. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions indicated that the new species grouped with Skeletocutis and nested in the tyromyces clade.

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Two new Leptobrachella species (Anura, Megophryidae) from the Yunnan-Guizhou Plateau, southwestern China

ZooKeys ◽

10.3897/zookeys.995.55939 ◽

2020 ◽

Vol 995 ◽

pp. 97-125

Author(s):

Jian Wang ◽

Zhi-Tong Lyu ◽

Shuo Qi ◽

Zhao-Chi Zeng ◽

Wen-Xiang Zhang ◽

...

Keyword(s):

Yunnan Province ◽

Nature Reserve ◽

Guizhou Province ◽

Morphological Data ◽

Southwestern China ◽

Forest Park ◽

Guizhou Plateau ◽

High Diversity

Two new toad species of the genus Leptobrachella are described from the Yunnan-Guizhou Plateau of China, based on the combination of molecular and morphological data. The description of Leptobrachella aspera Wang, Lyu, Qi & Wang, sp. nov. from Huanglianshan Nature Reserve represents the thirteenth Leptobrachella species known from Yunnan Province, and the description of Leptobrachella dorsospina Wang, Lyu, Qi & Wang, sp. nov. from Yushe Forest Park represents the sixth Leptobrachella species known from Guizhou Province. These new discoveries further emphasize the extremely high diversity of the Leptobrachella toads in these regions.

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Two new Chrysosporium (Onygenaceae, Onygenales) from China

Phytotaxa ◽

10.11646/phytotaxa.270.3.5 ◽

2016 ◽

Vol 270 (3) ◽

pp. 210 ◽

Cited By ~ 3

Author(s):

YAN-WEI ZHANG ◽

WANHAO CHEN ◽

GUIPING ZENG ◽

XIAO ZOU ◽

TINGCHI WEN ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Phylogenetic Tree ◽

Soil Samples ◽

The Other ◽

Guizhou Province ◽

Molecular Evidence ◽

5.8S Rdna ◽

Rdna Sequences ◽

Fungal Isolates

Fungal isolates GZUIFR-EM14.2002 and GZUIFR-EM66601 were respectively isolated from Chinese soil samples under the snake skin in Guizhou Province and from the soil samples under the feathers in Hubei Province, China. Morphological and molecular evidence support both isolates as new species of Chrysosporium. Phylogenetic analysis based on ITS-5.8S rDNA sequences grouped GZUIFR-EM14.2002 together with C. lucknowense and C. mephiticum. GZUIFR-EM14.2002, which could be distinguished from the latter two species by the presence of abundant intercalary conidia, was named C. guizhouense sp. nov. In the phylogenetic tree, GZUIFR-EM66601 was most closely related to C. submersum and C. siglerae, GZUIFR-EM66601 differed from the other two species in having small obovate to ellipsoidal conidia and no intercalary conidia; this strain was designated as C. hubeiense sp. nov. Holotypes and their isolates had been deposited in GZAC, Guiyang, Guizhou Province, China.

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Molecular Evidence for Association of Chlamydiales Bacteria with Epitheliocystis in Leafy Seadragon (Phycodurus eques), Silver Perch (Bidyanus bidyanus), and Barramundi (Lates calcarifer)

Applied and Environmental Microbiology ◽

10.1128/aem.72.1.284-290.2006 ◽

2006 ◽

Vol 72 (1) ◽

pp. 284-290 ◽

Cited By ~ 41

Author(s):

Adam Meijer ◽

Paul J. M. Roholl ◽

Jacobus M. Ossewaarde ◽

Brian Jones ◽

Barbara F. Nowak

Keyword(s):

Phylogenetic Analysis ◽

16S Rrna ◽

16S Rrna Gene ◽

Molecular Evidence ◽

Rrna Gene ◽

Lates Calcarifer ◽

The Family ◽

First Time ◽

Specific Sequences

ABSTRACT Epitheliocystis in leafy seadragon (Phycodurus eques), silver perch (Bidyanus bidyanus), and barramundi (Lates calcarifer), previously associated with chlamydial bacterial infection using ultrastructural analysis, was further investigated by using molecular and immunocytochemical methods. Morphologically, all three species showed epitheliocystis cysts in the gills, and barramundi also showed lymphocystis cysts in the skin. From gill cysts of all three species and from skin cysts of barramundi 16S rRNA gene fragments were amplified by PCR and sequenced, which clustered by phylogenetic analysis together with other chlamydia-like organisms in the order Chlamydiales in a lineage separate from the family Chlamydiaceae. By using in situ RNA hybridization, 16S rRNA Chlamydiales-specific sequences were detected in gill cysts of silver perch and in gill and skin cysts of barramundi. By applying immunocytochemistry, chlamydial antigens (lipopolysaccharide and/or membrane protein) were detected in gill cysts of leafy seadragon and in gill and skin cysts of barramundi, but not in gill cysts of silver perch. In conclusion, this is the first time epitheliocystis agents of leafy seadragon, silver perch and barramundi have been undoubtedly identified as belonging to bacteria of the order Chlamydiales by molecular methods. In addition, the results suggested that lymphocystis cysts, known to be caused by iridovirus infection, could be coinfected with the epitheliocystis agent.

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Youngia zhengyiana (Asteraceae, Crepidinae), a new species from south China, with notes on the systematics of Youngia inferred from morphology and nrITS phylogeny

Phytotaxa ◽

10.11646/phytotaxa.170.4.3 ◽

2014 ◽

Vol 170 (4) ◽

pp. 259 ◽

Cited By ~ 7

Author(s):

Tao Deng ◽

Jian Wen Zhang ◽

XIN XIN ZHU ◽

Dai Gui Zhang ◽

Zelong Nie ◽

...

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

South China ◽

Related Species ◽

Guizhou Province ◽

Molecular Phylogenetic Analysis ◽

Infrageneric Classification ◽

Molecular Phylogenetic ◽

First Time ◽

A New Species

Youngia zhengyiana, a new species of Youngia sect. Mesomeris (Asteraceae: Crepidinae) from Guizhou province of south China is described and illustrated. The placement of this species within Youngia is assessed based on a molecular phylogenetic analysis of the nuclear ribosomal ITS and on morphological comparisons with related species. The new species can be easily distinguished by morphology from the only species known to possess 5 florets, Y. szechuanica. The infrageneric classification and the recently debated circumscription of Youngia are discussed in the light of the nrITS phylogeny, which includes several species for the first time. Pseudoyoungia is confirmed as a congener of Youngia. The redefined Youngia is still non-monophyletic with Lapsanastrum nested within it.

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Two new species of Alseodaphnopsis (Lauraceae) from southwestern China and northern Myanmar: evidence from morphological and molecular analyses

PhytoKeys ◽

10.3897/phytokeys.138.38569 ◽

2020 ◽

Vol 138 ◽

pp. 27-39

Author(s):

Lang Li ◽

Yun-Hong Tan ◽

Hong-Hu Meng ◽

Hui Ma ◽

Jie Li

Keyword(s):

New Species ◽

Yunnan Province ◽

Conservation Status ◽

Iucn Red List ◽

Red List ◽

Southwestern China ◽

Molecular Evidence ◽

Molecular Analyses ◽

Iucn Red List Categories ◽

Two New Species

Alseodaphnopsis maguanensis and A. putaoensis, two new species of Alseodaphnopsis (Lauraceae) from southwestern China (Yunnan Province) and northern Myanmar (Kachin State), are here described and illustrated based on both morphological and molecular evidence. They are morphologically similar to Alseodaphnopsis rugosa and phylogenetically closely related to A. rugosa and A. hainanensis respectively. Their preliminary conservation status is also estimated according to the IUCN Red List Categories and Criteria.

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Steccherinum fragile sp. nov. and S. subcollabens comb. nov. (Steccherinaceae, Polyporales), evidenced by morphological characters and phylogenetic analysis

Phytotaxa ◽

10.11646/phytotaxa.483.2.3 ◽

2021 ◽

Vol 483 (2) ◽

pp. 106-116

Author(s):

ZHAN-BO LIU ◽

YU-CHENG DAI

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Yunnan Province ◽

Strong Support ◽

Morphological Characters ◽

New Combination ◽

Molecular Evidence ◽

Rdna Sequences ◽

Monophyletic Lineage ◽

The Family

A new poroid wood-inhabiting fungus in the family Steccherinaceae, Steccherinum fragile sp. nov., is described and illustrated from China based on morphological and molecular evidence. The species was collected in Yunnan Province, growing on the ground and undersides of rotten angiosperm wood. S. fragile is characterized by an annual growth habit, resupinate basidiocarps with salmon to peach pores when fresh becoming cinnamon buff to pinkish buff upon drying, a thin margin with distinct rhizomorphs, a monomitic hyphal system, generative hyphae thin- to distinctly thick-walled with simple septa, neither amyloid nor cyanophilous. The phylogenetic analysis based on ITS + nLSU rDNA sequences shows that the new species belongs to Steccherinum, forming a monophyletic lineage with strong support (100% BS, 100% BP, 1.00 BPP). In addition, a new combination, S. subcollabens, is also proposed based on examination of its type material and phylogenetic analysis. Phylogenetically, Steccherinum fragile is closely related to S. amapaense and groups with S. subcollabens, S. formosanum and S. collabens. Both morphological and molecular characters confirm the placement of the new species and combination in Steccherinum.

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