Effects of short-term administration of quinine on the seminiferous tubules of Sprague-Dawley Rats

AbstractChemopreventive effect of non-steroidal antiinflammatory drugs (NSAIDs) in mammary carcinogenesis was reported in several studies. In this study, the effect of a nonselective cyclooxygenase inhibitor diclofenac (DICLO) in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in Sprague-Dawley female rats was evaluated. NMU was administered to animals intraperitoneally in two doses of 50 mg kg−1 b.w. within postnatal days 42-48. In experiment A (short-term administration), DICLO was administrated intramuscularly (5 mg kg−1 b.w.) every other day, starting 3 days before and for subsequent 25 days after first NMU injection. In experiment B (long-term administration), DICLO was administered in tap water (0.01 mg ml−1) continually, starting 7 days before and for subsequent 22 weeks after first NMU dose. The study was terminated 22 weeks after the first dose of NMU in both experiments. After DICLO treatment, tumor frequency per group was reduced in both variants of drug administration: in experiment A by 38% and in experiment B by 39.5%. Moreover, DICLO decreased tumor incidence by 11.5% and delayed tumor latency by 14 days in experiment B. In our preventive-curative experiments DICLO decreased some parameters of NMU-induced rat mammary carcinogenesis, mainly the tumor frequency.

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Short-term administration of an aqueous extract of kalanchoe integra var. crenata (Andr.) Cuf leaves produces no major organ damage in Sprague-Dawley rats

Journal of Ethnopharmacology ◽

10.1016/j.jep.2013.11.050 ◽

2014 ◽

Vol 151 (2) ◽

pp. 891-896 ◽

Cited By ~ 2

Author(s):

Isaac J. Asiedu-Gyekye ◽

Daniel A. Antwi ◽

Charles Awortwe ◽

Benoit Banga N'guessan ◽

Alexander K. Nyarko

Keyword(s):

Aqueous Extract ◽

Organ Damage ◽

Sprague Dawley ◽

Short Term ◽

Sprague Dawley Rats ◽

Major Organ ◽

Term Administration

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Effect of a Short-Term and Long-Term Melatonin Administration on Mammary Carcinogenesis in Female Sprague-Dawley Rats Influenced by Repeated Psychoemotional Stress

Acta Veterinaria Brno ◽

10.2754/avb200776030371 ◽

2007 ◽

Vol 76 (3) ◽

pp. 371-377 ◽

Cited By ~ 2

Author(s):

M. Kassayová ◽

E. Adámeková ◽

B. Bojková ◽

P. Kubatka ◽

I. Ahlers ◽

...

Keyword(s):

Mammary Carcinogenesis ◽

Tumour Volume ◽

Female Rats ◽

Sprague Dawley ◽

Short Term ◽

Psychoemotional Stress ◽

Sprague Dawley Rats ◽

Melatonin Administration ◽

Term Administration

The aim of this study was to evaluate the effect of melatonin (MEL) on N-methyl-N-nitrosourea (NMU)-induced mammary carcinogenesis in female Sprague-Dawley rats exposed to repeated psychoemotional stress - immobilization in boxes. NMU was applied intraperitoneally in two doses each of 50 mg/kg b.w. between 40 - 50 postnatal days. Melatonin was administered in drinking water at a concentration of 4 μg/ml daily from 15:00 h to 8:00 h. The application was initiated 5 days prior to the fi rst NMU dose and lasted 15 days, i.e. during the promotion phase of tumour development, or long-term until the end of the experiment (week 20). Immobilization (2 h per day) began on the third day after the second carcinogen application and lasted for 7 consecutive days. Short-term MEL administration to immobilized animals increased incidence by 22%, decreased tumour frequency per animal by 26% and reduced tumour volume gain (by 21%) when compared to the immobilized group without MEL application. Decreased frequency per animal by 28% and more than a 40% decrease in tumour volume gain and cumulative volume were the most pronounced changes in the animals drinking MEL until the end of the experiment. Long-term MEL administration reduced the number and size of mammary tumours more markedly than its short-term administration. Melatonin decreased certain attributes of mammary carcinogenesis in female rats influenced by psychoemotional stress.

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ABSENCE OF VISIBLE TOXIC EFFECTS ACCOMPANYING THE SHORT-TERM ADMINISTRATION OF AN AQUEOUS EXTRACT OF ALLIUM SATIVA LINN. IN MALE SPRAGUE-DAWLEY RATS

American Journal of Pharmacology and Toxicology ◽

10.3844/ajptsp.2014.53.67 ◽

2014 ◽

Vol 9 (1) ◽

pp. 53-67 ◽

Cited By ~ 2

Author(s):

Isaac

Keyword(s):

Aqueous Extract ◽

Toxic Effects ◽

Sprague Dawley ◽

Short Term ◽

Sprague Dawley Rats ◽

Term Administration

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Evaluation of Reproductive Development Following Intravenous and Oral Exposure to DEHP in Male Neonatal Rats

International Journal of Toxicology ◽

10.1080/10915810305098 ◽

2003 ◽

Vol 22 (3) ◽

pp. 159-174 ◽

Cited By ~ 26

Author(s):

Jon N. Cammack ◽

Randy D. White ◽

Donovan Gordon ◽

Jerome Gass ◽

Lawrence Hecker ◽

...

Keyword(s):

Sperm Count ◽

Liver Weight ◽

Oral Exposure ◽

Seminiferous Tubules ◽

Primary Group ◽

Sprague Dawley ◽

Intravenous Injections ◽

Sprague Dawley Rats ◽

Recovery Group ◽

Ethylhexyl Phthalate

Di-(2-ethylhexyl)phthalate (DEHP) was administered to 3- to 5-day-old male Sprague-Dawley rats by daily intravenous injections of 60, 300, or 600 mg/kg/day or by daily oral gavage of 300 or 600 mg/kg/day for 21 days. Histopathological evaluation and organ weight measurements were performed on some animals after 21 days of dosing (primary group) and later on the recovery group animals that were held without further treatment until sexual maturity at approximately 90 days of age. No effects of any type were observed in animals treated intravenously with 60 mg/kg/day. Testicular changes, consisting of a partial depletion of the germinal epithelium and/or decrease in diameter of seminiferous tubules, were present in all animals of the 300- and 600-mg/kg/day groups after the 21-day dosing period. Testes weight decreased and liver weight increased in these animals. Testes changes were dose-related and generally more severe among animals dosed orally versus intravenously. In the recovery animals, a residual DEHP-induced decrease in seminiferous tubule diameter was present in the testis of several animals dosed orally at 300 and 600 mg/kg/day, but not in animals dosed intravenously. There was no germinal cell depletion or Sertoli cell alteration observed in any dose group at any time. Notably, no effects on sperm count, sperm morphology, or sperm motility were observed at 90 days of age in any of the groups.

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Short-Term High Fat Intake Does Not Significantly Alter Markers of Renal Function or Inflammation in Young Male Sprague-Dawley Rats

Journal of Nutrition and Metabolism ◽

10.1155/2015/157520 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Catherine Crinigan ◽

Matthew Calhoun ◽

Karen L. Sweazea

Keyword(s):

Kidney Disease ◽

Renal Mass ◽

Early Stage ◽

Young Male ◽

Protective Effects ◽

Sprague Dawley ◽

High Fat ◽

Short Term ◽

Sprague Dawley Rats ◽

The Impact

Chronic high fat feeding is correlated with diabetes and kidney disease. However, the impact of short-term high fat diets (HFD) is not well-understood. Six weeks of HFD result in indices of metabolic syndrome (increased adiposity, hyperglycemia, hyperinsulinemia, hyperlipidemia, hyperleptinemia, and impaired endothelium-dependent vasodilation) compared to rats fed on standard chow. The hypothesis was that short-term HFD would induce early signs of renal disease. Young male Sprague-Dawley rats were fed either HFD (60% fat) or standard chow (5% fat) for six weeks. Morphology was determined by measuring changes in renal mass and microstructure. Kidney function was measured by analyzing urinary protein, creatinine, and hydrogen peroxide (H2O2) concentrations, as well as plasma cystatin C concentrations. Renal damage was measured through assessment of urinary oxDNA/RNA concentrations as well as renal lipid peroxidation, tumor necrosis factor alpha (TNFα), and interleukin 6 (IL-6). Despite HFD significantly increasing adiposity and renal mass, there was no evidence of early stage kidney disease as measured by changes in urinary and plasma biomarkers as well as histology. These findings suggest that moderate hyperglycemia and inflammation produced by short-term HFD are not sufficient to damage kidneys or that the ketogenic HFD may have protective effects within the kidneys.

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